LOCUS       sequence-170081-        8330 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lenti reporter plasmid with TTR_V30M-specific gRNA target-sequence, 
            to be used with tGFP #26864.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 8330)
  AUTHORS   Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, 
            Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher 
            M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, 
            Cathomen T, Cantz T
  TITLE     Improved bi-allelic modification of a transcriptionally silent locus
            in patient-derived iPSC by Cas9 nickase.
  JOURNAL   Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
  PUBMED    27910942
REFERENCE   2  (bases 1 to 8330)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-2"; volume: "6:38198. doi"; pages: " 
            10.1038/srep38198"
COMMENT     Sequence Label: Lenti CGIP + TTR target
FEATURES             Location/Qualifiers
     source          1..8330
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      56..65
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(107..128)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(368..387)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     855..876
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(961..1535,1536..1538,1539..1547)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1128..1145)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1355..1374
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             1548..2147
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1548..1567)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2044..2064
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     complement(2203..2219)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2204..2220)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    2257..2845
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2310..2330)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2728..2739)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2932..3165
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3237..3358
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3274..3293)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3328..3347
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3377..3512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3544..3563)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3545..3563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3573..3590)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3573..3589)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3582..3604)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3734..4189
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3821..3840)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4031..4052
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4218..4322
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4323..4391,4392..5183)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4541..4560)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5354..5942
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5843..5862
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6096..6113
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6230..6251
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6266..6271,6272..6289,6290..6296)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6304..6320
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6309..6331
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6328..6344
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6328..6344
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6363..6383
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6365..6383
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     LTR             6639..6819
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6863..6988
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7481..7714
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7899..7943
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8210..8327
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tggttattgt gctgtctcat cattttggca aagaattcct cgagatccac cgggcgccac
       61 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
      121 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      181 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      241 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      301 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      361 cttcaaggac gacggcaact acaagaccta acccagggca ccggtgaatc caagtgtcct
      421 ctgatggtca aagttctaga tgctgtccga ggcagtcctg ccatcaatgt ggccatgcat
      481 gtgttcagaa aggctgctga tgacacctgg gagccatttg cctctgggcg cgccgaggtg
      541 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      601 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      661 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      721 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      781 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac
      841 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
      901 atggacgagc tgtacaagta aagcggccgc gtcgacgggc ccgcggaatt ccgccccccc
      961 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     1021 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     1081 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     1141 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     1201 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     1261 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     1321 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     1381 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     1441 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     1501 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg accgagtaca
     1561 agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg
     1621 ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc
     1681 gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt
     1741 gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg
     1801 gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg
     1861 cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc
     1921 tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc
     1981 tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc
     2041 cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc
     2101 ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg
     2161 acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcgataccg tcgacctcga
     2221 gggggggccc ggtacaagta aagcggccgc gtcgacaatc aacctctgga ttacaaaatt
     2281 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2341 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2401 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2461 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2521 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2581 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2641 ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac ctggattctg
     2701 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2761 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2821 atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta agaccaatga
     2881 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2941 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3001 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     3061 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     3121 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc
     3181 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     3241 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     3301 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     3361 atgtctggct ctagctatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     3421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     3481 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcgtcgaga cgtacccaat
     3541 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3601 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3661 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3721 ggcgaatggc gcgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     3781 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     3841 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     3901 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     3961 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     4021 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     4081 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     4141 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc ccaggtggca
     4201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     4261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     4321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     5161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     5221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     5281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     5341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5461 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     5521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6001 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     6061 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     6121 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     6181 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     6241 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     6301 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     6361 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     6421 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     6481 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     6541 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     6601 agatattgta tttaagtgcc tagctcgata caataaacgg gtctctctgg ttagaccaga
     6661 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     6721 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     6781 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggacct
     6841 gaaagcgaaa gggaaaccag agctctctcg acgcaggact cggcttgctg aagcgcgcac
     6901 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     6961 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     7021 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     7081 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     7141 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     7201 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     7261 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     7321 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7381 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     7441 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     7501 tcttgggagc agcaggaagc actatgggcg cagcctcaat gacgctgacg gtacaggcca
     7561 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     7621 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     7681 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     7741 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     7801 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     7861 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     7921 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     7981 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8041 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8101 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8161 gagacagatc cattcgatta gtgaacggat ctcgacggta tcggttaact tttaaaagaa
     8221 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
     8281 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttatc
//
