LOCUS       sequence-170125-        9464 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Sensor Plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Mullokandov G, Baccarini A, Ruzo A, Jayaprakash AD, Tung N, Israelow
            B, Evans MJ, Sachidanandam R, Brown BD
  TITLE     High-throughput assessment of microRNA activity and function using 
            microRNA sensor and decoy libraries.
  JOURNAL   Nat Methods. 2012 Jul 1;9(8):840-6. doi: 10.1038/nmeth.2078.
  PUBMED    22751203
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-07-1- 1"; volume: "9"; 
            issue: "8"; pages: "840-6"
COMMENT     Sequence Label: 
            AB.pCCL.sin.cPPT.GFP.miR-138-2-3p.sensor.PGK.dNGFR.WPRE
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(1..511)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(64..82)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     promoter        539..577
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             join(675..677,678..680,681..1394)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(675..677,678..680,681..1391)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(720..741)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(981..1000)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1328..1349
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1671..1805
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    1942..2059
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(2326..2370)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2555..2788
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3281..3406
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3453..3633
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(3648..3851)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     enhancer        3852..4231
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(4332..4352)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4332..4350)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4371..4387)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4371..4387)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4384..4406)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4395..4411
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4419..4425,4426..4443,4444..4449))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4464..4485
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4602..4619)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4773..5361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4853..4872)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5532..6323,6324..6392))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6155..6174
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6393..6497)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6523..6978)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6660..6681)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6872..6891
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7105..7127
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7119..7136
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7120..7136
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7146..7165
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7146..7164
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7185..7320
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7360..7481
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     LTR             7553..7786
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    7873..8461
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7979..7990
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     8388..8408
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ctggggagag aggtcggtga ttcggtcaac gagggagccg actgccgacg tgcgctccgg
       61 aggcttgcag aatgcggaac accgcgcggg caggaacagg gcccacacta ccgccccaca
      121 ccccgcctcc cgcaccgccc cttcccggcc gctgctctcg gcgcgccctg ctgagcagcc
      181 gctattggcc acagcccatc gcggtcggcg cgctgccatt gctccctggc gctgtccgtc
      241 tgcgagggta ctagtgagac gtgcggcttc cgtttgtcac gtccggcacg ccgcgaaccg
      301 caaggaacct tcccgactta ggggcggagc aggaagcgtc gccggggggc ccacaagggt
      361 agcggcgaag atccgggtga cgctgcgaac ggacgtgaag aatgtgcgag acccagggtc
      421 ggcgccgctg cgtttcccgg aaccacgccc agagcagccg cgtccctgcg caaacccagg
      481 gctgccttgg aaaaggcgca accccaaccc cgtggaattc gatatcaagc ttctcgaggg
      541 taggcgtgta cggtgggagg cctatataag cagagctcgt ttagtgaacc gtcagatcgc
      601 ctggagacgc catccacgct gttttgacct ccatagaaga caccgggacc gatccagcct
      661 ccgcggaatt caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
      721 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
      781 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
      841 tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc
      901 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
      961 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     1021 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     1081 acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg
     1141 acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca
     1201 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     1261 tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc aacgagaagc
     1321 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     1381 agctgtacaa gtaaagcggc cgcgtcgaga gatgggggag gctaactgag ctagccgacc
     1441 ttaactgggg agtactggtc gacgtagcaa ccctggtgtc gtgaaatagc aaccctggtg
     1501 tcgtgaaata gcaaccctgg tgtcgtgaaa tagcaaccct ggtgtcgtga aatagcaacc
     1561 ctggtgtcgt gaaatagcaa ccctggtgtc gtgaaatagc accggtacta gcttagtcat
     1621 gcaccgggct gcaggaattc gatgacaggc gcagcttccg agggatttga gatccagaca
     1681 tgataagata cattgatgag tttggacaaa ccaaaactag aatgcagtga aaaaaatgcc
     1741 ttatttgtga aatttgtgat gctattgcct tatttgtaac cattataagc tgcaataaac
     1801 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     1861 ttttttaaag caagtaaaac ctctacaaat gtggtatggc tgattatgat cagatctctc
     1921 gaggctagtc tcgtgatcga taaaattttg aatttttgta atttgttttt gtaattcttt
     1981 agtttgtatg tctgttgcta ttatgtctac tattctttcc cctgcactgt accccccaat
     2041 cccccctttt cttttaaaag ttaaccgata ccgtcgagat ccgttcacta atcgaatgga
     2101 tctgtctctg tctctctctc caccttcttc ttctattcct tcgggcctgt cgggtcccct
     2161 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     2221 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     2281 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     2341 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     2401 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     2461 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     2521 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     2581 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     2641 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     2701 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     2761 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     2821 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     2881 atataattca cttctccaat tgtccctcat atctcctcct ccaggtctga agatcagcgg
     2941 ccgcttgctg tgcggtggtc ttacttttgt tttgctcttc ctctatcttg tctaaagctt
     3001 ccttggtgtc ttttatctct atcctttgat gcacacaata gagggttgct actgtattat
     3061 ataatgatct aagttcttct gatcctgtct gaagggatgg ttgtagctgt cccagtattt
     3121 gtctacagcc ttctgatgtt tctaacaggc caggattaac tgcgaatcgt tctagctccc
     3181 tgcttgccca tactatatgt tttaatttat attttttctt tccccctggc cttaaccgaa
     3241 ttttttccca tcgcgatcta attctccccc gcttaatact gacgctctcg cacccatctc
     3301 tctccttcta gcctccgcta gtcaaaattt ttggcgtact caccagtcgc cgcccctcgc
     3361 ctcttgccgt gcgcgcttca gcaagccgag tcctgcgtcg agagagctcc tctggtttcc
     3421 ctttcgcttt caagtccctg ttcgggcgcc actgctagag attttccaca ctgactaaaa
     3481 gggtctgagg gatctctagt taccagagtc acacaacaga cgggcacaca ctacttgaag
     3541 cactcaaggc aagctttatt gaggcttaag cagtgggttc cctagttagc cagagagctc
     3601 ccaggctcag atctggtcta accagagaga ccccggttca ctaaacgagc tctgcttata
     3661 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
     3721 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
     3781 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
     3841 aaccgcatca ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     3901 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     3961 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     4021 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     4081 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     4141 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     4201 tactattaat aactagtcaa taatcaatgt caacatggcg gtaatgttgg acatgagcca
     4261 atataaatgt acatattatg atatggatac aacgtatgca atggccaagc ttgcagctcc
     4321 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
     4381 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4441 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4501 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4561 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4621 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4681 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4741 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4801 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4861 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4921 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4981 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     5041 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     5101 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     5161 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     5221 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     5281 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5341 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5401 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5461 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5521 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5581 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5641 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5701 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5761 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5821 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5881 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5941 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     6001 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     6061 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     6121 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     6181 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     6241 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6301 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6361 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6421 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6481 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     6541 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     6601 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     6661 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     6721 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     6781 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     6841 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     6901 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     6961 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     7021 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     7081 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     7141 gcgcgtaata cgactcacta tagggcgaat tgggtacgtc cctaggcctc caaaaaagcc
     7201 tcctcactac ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa
     7261 aaaaattagt cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat
     7321 gggcggagtt aggggcggga tagctagagc cagacatgat aagatacatt gatgagtttg
     7381 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     7441 ttgctttatt tgtaaccatt ataagctgca ataaacaagt tcctctcact ctctgatatt
     7501 catttctttg caagttataa atactgaata ataagatgac atgaactact actgctagag
     7561 attttccaca ctgactaaaa gggtctgagg gatctctagt taccagagtc acacaacaga
     7621 cgggcacaca ctacttgaag cactcaaggc aagctttatt gaggcttaag cagtgggttc
     7681 cctagttagc cagagagctc ccaggctcag atctggtcta accagagaga cccagtacaa
     7741 gcaaaaagca gatcttgtct tcgttgggag tgaattagcc cttccagtcc ccccttttct
     7801 tttaaaaagt ggctaagatc tacagctgcc ttgtaagtca ttggtcttaa aggtaccgag
     7861 ctcgaattcc aggcggggag gcggcccaaa gggagatccg actcgtctga gggcgaaggc
     7921 gaagacgcgg aagaggccgc agagccggca gcaggccgcg ggaaggaagg tccgctggat
     7981 tgagggccga agggacgtag cagaaggacg tcccgcgcag aatccaggtg gcaacacagg
     8041 cgagcagcca tggaaaggac gtcagcttcc ccgacaacac cacggaattg tcagtgccca
     8101 acagccgagc ccctgtccag cagcgggcaa ggcaggcggc gatgagttcc gccgtggcaa
     8161 tagggagggg gaaagcgaaa gtcccggaaa ggagctgaca ggtggtggca atgccccaac
     8221 cagtgggggt tgcgtcagca aacacagtgc acaccacgcc acgttgcctg acaacgggcc
     8281 acaactcctc ataaagagac agcaaccagg atttatacaa ggaggagaaa atgaaagcca
     8341 tacgggaagc aatagcatga tacaaaggca ttaaagcagc gtatccacat agcgtaaaag
     8401 gagcaacata gttaagaata ccagtcaatc tttcacaaat tttgtaatcc agaggttgat
     8461 tgtcgactct agactcgact ctagaggatc cccctgttcc acctcttgaa ggctatgtag
     8521 gccacaaggc ccacaaccac agcagccagg atggagcaat agacagggat gaggttgtcg
     8581 gtggtgcctc gggtcaccac gggctgggag ctgcccatca ctgtggtcac cacacctgcc
     8641 accgtgctgg ctatgaggtc ttgttctgga ggtgcctcag gctcctgggt gctgggggct
     8701 gtgctgtccg agccctctgg gggtgtggac cgtgtaatcc aacggccagg gatctcctcg
     8761 cactcggcgt cggcccagcg tgtgcactcg cggagctggc gctcggtgtc ctcgcacacg
     8821 gtgcagggca ggcacgggtc cacgtggttg gcctcgtcgg aatacgtgcc gtcggggcac
     8881 tcctcgcaca cggtgttctg cttgtcctgg caggagaaca cgaggcccga gcccgcctcg
     8941 cacacgcggc acgcctcgca gcgcccagtc gtctcatcct ggtagtagcc gtaggcgcag
     9001 cggcacacgg cgtcgtcggc ctccacgcac ggcgccgaca tgctctggag ccccacgcac
     9061 tcggtgcacg gcttgcacgg ctcggtcgcg ctcaccacgt cggagaacgt cacgctgtcc
     9121 aggcagggct cacacacggt ctggttggct ccacaaggct gggccacacc ctcgcccagg
     9181 ttgcaggctt tgcagcactc accgctgtgt gtgtacaggc ctgtggggca tgcctccttg
     9241 gcacctccaa gggacacccc cagaagcagc aacagcagca ggcgcggccc gtccatggcg
     9301 cggccggtgg cacctgcccc catcgcccgc ctcccgcggc agcgctcgac ttccagctcg
     9361 gtccgctttg cggactgatg gggctgcgct gcgctgcgct ccagcgcccc ccctgcccgc
     9421 cggagctggc cgcggcccga attcctgcag cccgggggat cccc
//
