LOCUS       sequence-170163-         221 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  disruption of human RMRP gene via CRISPR-Cas9, guide 4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 221)
  AUTHORS   Goldfarb KC, Cech TR
  TITLE     Targeted CRISPR disruption reveals a role for RNase MRP RNA in human
            preribosomal RNA processing.
  JOURNAL   Genes Dev. 2017 Jan 1;31(1):59-71. doi: 10.1101/gad.286963.116. Epub
            2017 Jan 23.
  PUBMED    28115465
REFERENCE   2  (bases 1 to 221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.286963.116"; journalName: "Genes Dev"; date: 
            "2017-01-1- 1"; volume: "31"; issue: "1"; pages: "59-71"
COMMENT     Sequence Label: px330-MRPsg4
FEATURES             Location/Qualifiers
     source          1..221
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     163..182
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 tttcccatga ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga
       61 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
      121 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      181 gtaacttgaa agtatttcga tttcttggct ttatatatct t
//
