LOCUS       sequence-170197-        9268 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ATR minigene to test splicing event.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9268)
  AUTHORS   Ilagan JO, Ramakrishnan A, Hayes B, Murphy ME, Zebari AS, Bradley P,
            Bradley RK
  TITLE     U2AF1 mutations alter splice site recognition in hematological 
            malignancies.
  JOURNAL   Genome Res. 2015 Jan;25(1):14-26. doi: 10.1101/gr.181016.114. Epub 
            2014 Sep 29.
  PUBMED    25267526
REFERENCE   2  (bases 1 to 9268)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gr.181016.114"; journalName: "Genome Res"; date: "2015-01";
            volume: "25"; issue: "1"; pages: "14-26"
COMMENT     Sequence Label: pUB6_ATR
FEATURES             Location/Qualifiers
     source          1..9268
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..1227
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1064..1083
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     CDS             5132..5173
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             5183..5200
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(5223..5240)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5229..5453
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5499..5927
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5586..5605)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5796..5817
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5936..5956)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5941..6271
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6122..6257
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6184..6203
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6319..6366
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6385..6783
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     polyA_signal    6941..7062
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6978..6997)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7032..7051
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7111..7127)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7111..7127)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7124..7146)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7135..7151
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7159..7165,7166..7183,7184..7189))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7204..7225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7342..7359)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7513..8101)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7593..7612)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8272..9063,9064..9132))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8895..8914
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9133..9237)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctggc ctccgcgccg ggttttggcg cctcccgcgg gcgcccccct
       61 cctcacggcg agcgctgcca cgtcagacga agggcgcagg agcgtcctga tccttccgcc
      121 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
      181 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
      241 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
      301 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
      361 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggtgagt
      421 agcgggctgc tgggctggcc ggggctttcg tggccgccgg gccgctcggt gggacggaag
      481 cgtgtggaga gaccgccaag ggctgtagtc tgggtccgcg agcaaggttg ccctgaactg
      541 ggggttgggg ggagcgcagc aaaatggcgg ctgttcccga gtcttgaatg gaagacgctt
      601 gtgaggcggg ctgtgaggtc gttgaaacaa ggtggggggc atggtgggcg gcaagaaccc
      661 aaggtcttga ggccttcgct aatgcgggaa agctcttatt cgggtgagat gggctggggc
      721 accatctggg gaccctgacg tgaagtttgt cactgactgg agaactcggt ttgtcgtctg
      781 ttgcgggggc ggcagttatg cggtgccgtt gggcagtgca cccgtacctt tgggagcgcg
      841 cgccctcgtc gtgtcgtgac gtcacccgtt ctgttggctt ataatgcagg gtggggccac
      901 ctgccggtag gtgtgcggta ggcttttctc cgtcgcagga cgcagggttc gggcctaggg
      961 taggctctcc tgaatcgaca ggcgccggac ctctggtgag gggagggata agtgaggcgt
     1021 cagtttcttt ggtcggtttt atgtacctat cttcttaagt agctgaagct ccggttttga
     1081 actatgcgct cggggttggc gagtgtgttt tgtgaagttt tttaggcacc ttttgaaatg
     1141 taatcatttg ggtcaatatg taattttcag tgttagacta gtaaattgtc cgctaaattc
     1201 tggccgtttt tggctttttt gttagacgaa gcttggtacc gagctcggat ccactagtcc
     1261 agtgtggtgg aattctgcag attgtcttaa agacttttct acatgatcct cttgtggaat
     1321 ggagtaaacc agtgaaaggg cattccaaag cgccactgaa tgaaactgga gaagttgtca
     1381 atgaaaaggt tagtagaagg tatagcttga tatgggctgt agtcctcatc tctacaaatg
     1441 aacttgatat gcagctatag ttctcatctc tatgcatgag aactgcttgg aaatactgtt
     1501 agtgactatt gattactctg aaatctagtt ctctagtatc acaatgccat aagttttctt
     1561 tattgtcagt aatgttaaca gtattcgcat ttgtataata aaagttcatg gcacacttgt
     1621 ttaaccatag acaattcaca gttatgagat acagtaggaa aaactatagt acaaattgcc
     1681 tgaacttaca gaacaacagg tctgagcttt aatatcatga taccagtatg tcacaagcta
     1741 tgttaacaaa tgcagcaatt cacttcttac ttagttgaag ctgcaaagtt tgcagatagt
     1801 tttgttgttg ttgttgtggt ggtggtggtg gttgttgttt ttgagacaga gtctcgctct
     1861 gtcatccagg ctggagtaca gtggcacgat cttggctcac ggcaacctct gcctcctggg
     1921 ttcaagcagt tctcctgcct tagcctccca agtagctggg attataagcg tgcaccacca
     1981 cagctggcta attattatat ttttagtaga gatgggtttc accatgttgg ccaggctggt
     2041 cttgaacccc tggcttcaag tgatccaccc acctcagcat cccaaaatgc agggattata
     2101 ggcataagcc actgtgccta gctcagatag ttgtttttaa taagttagaa tagattgagg
     2161 attcgttttt tttggtgggg ggcactctta catctttaat aagtcttgta caggcttact
     2221 aaatacaatg aattatacca actatatgct aataaagatt aaaaatttac attccagtta
     2281 actacctaaa gagtttaagt ccactttcat cctactgata tagctcgagg aaggctagac
     2341 ctgtacgccc ccatccttca tgtcatcctt aaccctttgt cttgagaaag aaggtttagc
     2401 tgcttcttta gttcttctaa aagaactaaa agaaaaattt ctttttcttg ctctattatc
     2461 ttcatctgta gggtgagatt aatatttcca gaattgtgga aaaattcact ctgtagcagt
     2521 tgaagagcag atggtctctg ggacatgttc cttttcgtta agtgatagat atacttggct
     2581 tgtactggat accttttact gagggattcc aatatctgac cagttcttga acctgttaaa
     2641 acttctgctc gctccatttc tgttctaaac agctgaaaga gctctagcag gatcacactc
     2701 aagctatata catatctaac tggacaaagc cagccctggt ctctacaaat agtttttgtt
     2761 tttttttaat tagctgggct cagtggcaca ctcctgtagt cccagctact tgggaggctg
     2821 aggtgggagg atcacttgac cccaggagtt ggaggctgct gtgagctatg atgacaccac
     2881 tgcactccag cctgggcaac agagtgagac ccttggcatc atactcagat ccttccaact
     2941 gctgggatga agcatacaga caagtgccca ctctggatat aggtgttggt gttctctttc
     3001 tgtttctatg ggtccagtct gtgttctttt gtaagatgtc tgggcaagcc agaccaaagt
     3061 ctcctatttt tacttgctga tcagggccat gaagaaaaat atttctaggc ttcagatctc
     3121 tgtttacaat acccatgtta tgtatgtaaa acacaccttc cactaaatct tgaaaatttt
     3181 ttgttgcaac actggccata aggacaggca aacttgccca cacactcttg gccctgtttg
     3241 tttctctcag ctagccagtc ccacagctgg agcttgcaca cctgcatctg gatgtgcagc
     3301 atcaggtggt actgcacctc gatccccaac aagttgaagt tttctttgga agattcctca
     3361 gtggatgtga aattctcctc taggagggaa ttacacctga gcagcagggg ctgtttcaca
     3421 attgaccagg tagacaaacc agccaggcca ttttcctgga gctccaggga tgattcatat
     3481 tcactggtgt ctcttaagac ggaattgatg gtgtagttca cagcttgttg ttctgatttt
     3541 gagtgtcaga ttctgcaaag ggtttttctt ttctgaggtg agctccgcga agataatggg
     3601 tgagctgcta ttttcaccat ttttaacacc gtattggtat ctgtctgctt ggtcggagaa
     3661 cacttccaga aatggcaact gaacggaagc tctgtctgct tgtggttgga ccacttgaac
     3721 atgttctgtc cacgcagtgt gataacgaat gatattaggg tgctgaagac ctgccagcac
     3781 cttcattccc cgtagttctt tcatgtaatg tgtttttgtt gcacccttaa tcaggatttt
     3841 ttaaattgca taatactgac catctaattt attcctgacc ttgtatactc ttccatatcc
     3901 accttttcct aagatggcaa gttcttcaaa ttcatttaag taacgtgaag tttgtgcttc
     3961 aaagggtact tcccttgatc tgattttctg gatatgagaa atagcctcac aaggatcctg
     4021 atgaactctc tctttagtgg acctcattaa gtgagtaata gctctgtggt gatgtagtct
     4081 caatgagcta aacttgtcac aagtgaaaga agacagcagc cccattttga taaaggtctg
     4141 gcaaagtaac ttaaacactg tcttgaatga actgggtttg gttcacgcac atggctcaag
     4201 tgcttcacca aagaaataag cggcagctgg tttgcaactg caaaagggaa agttggctgt
     4261 tgtaggggtt attttaatac ctggagttct gctggaacat aagtcatatt tggagtccag
     4321 gctcttggcg gaaaagtcga tggcagatgg cacagcaaca gtctccaccc cgtggccctc
     4381 ctctttgtgc tcgtgggctg cagcgcagcc caccagccca cccagcacca cctctgagaa
     4441 ttattcttct aataacgtta ttcttccttt tgcattcaga tatgcttaag aaattgtaca
     4501 gttccaagta gctcacttag aacacccatt tgcatgtact aacataatgt aaatattagg
     4561 cagtacagca ttgttatttg aatattcatt gaaatgttta agtcctaaac acttccaaag
     4621 cagatttaaa aaattatttt tctagaattg taatgtgtaa ataattccct ggtatttcac
     4681 gtagtccaga aacatgtcca agcagttgat gttgaatcaa tcttgcgagc ctccaaatca
     4741 gtatcacaag ctaactgtat ttgtttaatt tcttcttttc ctgtatttgc tcttacaaaa
     4801 ggggtattgg tcagtaaaat ggtattaaca tttattaaat gtcaaaatat aaagaaaggt
     4861 agtaattcca aattattaac atctgttttt ggttttatgt ttcttctttt taggccaaga
     4921 cccatgttct tgacattgag cagcgactac aaggtgtaat caagactcga aatagagtga
     4981 caggactgcc gttatctatt gaaggacatg tgcattacct tatacaagaa gctactgatg
     5041 aaaacttact atgccagatg tatcttggtt ggactccata tatgtgaatc cagcacagtg
     5101 gcggccgctc gagtctagag ggcccttcga aggtaagcct atccctaacc ctctcctcgg
     5161 tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa cccgctgatc
     5221 agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc
     5281 cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc
     5341 gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg acagcaaggg
     5401 ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta tggcttctga
     5461 ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta gcggcgcatt
     5521 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     5581 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     5641 agctctaaat cggggcatcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     5701 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     5761 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     5821 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgg ggatttcggc
     5881 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat tctgtggaat
     5941 gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag
     6001 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     6061 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     6121 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     6181 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     6241 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6301 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     6361 tacgacaagg tgaggaacta aaccatggcc aagcctttgt ctcaagaaga atccaccctc
     6421 attgaaagag caacggctac aatcaacagc atccccatct ctgaagacta cagcgtcgcc
     6481 agcgcagctc tctctagcga cggccgcatc ttcactggtg tcaatgtata tcattttact
     6541 gggggacctt gtgcagaact cgtggtgctg ggcactgctg ctgctgcggc agctggcaac
     6601 ctgacttgta tcgtcgcgat cggaaatgag aacaggggca tcttgagccc ctgcggacgg
     6661 tgccgacagg tgcttctcga tctgcatcct gggatcaaag ccatagtgaa ggacagtgat
     6721 ggacagccga cggcagttgg gattcgtgaa ttgctgccct ctggttatgt gtgggagggc
     6781 taagcacttc gtggccgagg agcaggactg acacgtgcta cgagatttcg attccaccgc
     6841 cgccttctat gaaaggttgg gcttcggaat cgttttccgg gacgccggct ggatgatcct
     6901 ccagcgcggg gatctcatgc tggagttctt cgcccacccc aacttgttta ttgcagctta
     6961 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7021 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     7081 gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     7141 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     7201 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
     7261 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
     7321 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7381 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7441 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7501 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7561 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7621 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7681 cccttcggga agcgtggcgc tttctcaatg ctcacgctgt aggtatctca gttcggtgta
     7741 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     7801 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     7861 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     7921 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     7981 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8041 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8101 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8161 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     8221 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     8281 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     8341 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     8401 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     8461 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     8521 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     8581 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     8641 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     8701 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     8761 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     8821 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     8881 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     8941 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     9001 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     9061 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     9121 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     9181 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     9241 atttccccga aaagtgccac ctgacgtc
//
