LOCUS       sequence-170198-       13813 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MESA target chain with V1-MESA ectodomain, 45 extracellular linkers,
            a flag tag, M cleavage sequence, and dCas9-VP64.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13813)
  AUTHORS   Schwarz KA, Daringer NM, Dolberg TB, Leonard JN
  TITLE     Rewiring human cellular input-output using modular extracellular 
            sensors.
  JOURNAL   Nat Chem Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253.
  PUBMED    27941759
REFERENCE   2  (bases 1 to 13813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Dec 12. doi: 10.1038/nchembio.2253."
COMMENT     Sequence Label: V1-MESA-45F-M-dCas9
FEATURES             Location/Qualifiers
     source          1..13813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(75..94)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        266..645
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        647..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     800..820
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             863..1043
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1090..1215
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1708..1941
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2126..2170
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2468..2585
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             3601..3621
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             3637..3684
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             join(3685..3708,3709..3711,3712..6267,6268..6270,
                     6271..7785)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the N863A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKARGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             7840..7860
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             7885..8034
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             8056..8109
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             8110..8508
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8533..9121
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8586..8606)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9004..9015)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(9124..9140)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9125..9141)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             9646..9826
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(9852..9869)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    9858..10082
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10128..10556
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10215..10234)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10425..10446
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10565..10585)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10570..10899
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10750..10885
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10812..10831
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        10947..10994
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11013..11387
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11517..11638
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11554..11573)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11608..11627
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11687..11703)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11687..11703)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11700..11722)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11711..11727
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11735..11741,11742..11759,11760..11765))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11780..11801
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(11918..11935)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12089..12677)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12169..12188)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12848..13639,13640..13708))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13471..13490
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13709..13813)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
       61 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
      121 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
      181 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
      241 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
      301 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      361 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      421 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      481 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      541 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      601 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      661 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      721 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      781 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      841 agcagcgcgt tttgcctgta ctgggtctct ctggttagac cagatctgag cctgggagct
      901 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
      961 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     1021 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     1081 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     1141 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     1201 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     1261 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     1321 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     1381 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     1441 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     1501 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     1561 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     1621 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     1681 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     1741 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     1801 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     1861 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     1921 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     1981 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     2041 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     2101 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     2161 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     2221 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     2281 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     2341 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     2401 tcgattagtg aacggatcgg cactgcgtgc gccaattctg cagacaaatg gcagtattca
     2461 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
     2521 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
     2581 attttcgggt ttattacagg gacagcagag atccagtttg gttaattagc tagcgccatc
     2641 atggcttgga ccagcttaat tcttagcttg cttgccctgt gcagcggtgc atcatctgac
     2701 atacagatga ctcagtcacc tagcagtctg tctgcttcag tcggtgatag ggtaactatc
     2761 acctgtcggg catctcagga tgtgtccacg gcagttgcct ggtaccagca gaagcccggc
     2821 aaggcgccta agttgcttat ctattcagct tcatttttgt atagtggggt gcctagccgg
     2881 tttagcggaa gtggttctgg cacagacttc actttgacaa tcagttccct ccaaccggag
     2941 gatttcgcta cctactactg tcagcagggg tacggaaacc cctttacgtt tggacagggc
     3001 actaaagtgg agattaagag gggaggcgga ggctccggtg ggggaggctc tggtggtgga
     3061 ggtagcgagg ttcagctggt ggaaagtggt ggcggactag tacagccagg cggctccctg
     3121 cggctgagtt gcgccgcgag tggctttact atatctgact attggatcca ttgggtcagg
     3181 caagcccctg ggaaaggatt ggagtgggtt gctggtatta cccccgcagg tgggtatact
     3241 tattatgcag atagcgtgaa agggaggttc accatctctg cagacacaag caagaacacg
     3301 gcctacctac agatgaactc tctgagggca gaggacactg ccgtctacta ttgcgcccga
     3361 tttgtgtttt ttctacccta cgccatggac tattggggac aggggacatt ggtaggcgcc
     3421 gggacaggag ggtctggggg tggcactggc ggatctggag gagactacaa ggacgatgac
     3481 gacaacggcg gatcgggggg gtctggtggt agtggcggtt cggggggagg taccggtctg
     3541 gtcgtggttg ctggagtcct gttttgttat ggcttgctag tgacagtggc tctttgtgtt
     3601 gaaaacctgt attttcagat gcgtacggcc accatgaaaa ggccggcggc cacgaaaaag
     3661 gccggccagg caaaaaagaa aaaggacaag aagtacagca tcggcctggc catcggcacc
     3721 aactctgtgg gctgggccgt gatcaccgac gagtacaagg tgcccagcaa gaaattcaag
     3781 gtgctgggca acaccgaccg gcacagcatc aagaagaacc tgatcggagc cctgctgttc
     3841 gacagcggcg aaacagccga ggccacccgg ctgaagagaa ccgccagaag aagatacacc
     3901 agacggaaga accggatctg ctatctgcaa gagatcttca gcaacgagat ggccaaggtg
     3961 gacgacagct tcttccacag actggaagag tccttcctgg tggaagagga taagaagcac
     4021 gagcggcacc ccatcttcgg caacatcgtg gacgaggtgg cctaccacga gaagtacccc
     4081 accatctacc acctgagaaa gaaactggtg gacagcaccg acaaggccga cctgcggctg
     4141 atctatctgg ccctggccca catgatcaag ttccggggcc acttcctgat cgagggcgac
     4201 ctgaaccccg acaacagcga cgtggacaag ctgttcatcc agctggtgca gacctacaac
     4261 cagctgttcg aggaaaaccc catcaacgcc agcggcgtgg acgccaaggc catcctgtct
     4321 gccagactga gcaagagcag acggctggaa aatctgatcg cccagctgcc cggcgagaag
     4381 aagaatggcc tgttcggcaa cctgattgcc ctgagcctgg gcctgacccc caacttcaag
     4441 agcaacttcg acctggccga ggatgccaaa ctgcagctga gcaaggacac ctacgacgac
     4501 gacctggaca acctgctggc ccagatcggc gaccagtacg ccgacctgtt tctggccgcc
     4561 aagaacctgt ccgacgccat cctgctgagc gacatcctga gagtgaacac cgagatcacc
     4621 aaggcccccc tgagcgcctc tatgatcaag agatacgacg agcaccacca ggacctgacc
     4681 ctgctgaaag ctctcgtgcg gcagcagctg cctgagaagt acaaagagat tttcttcgac
     4741 cagagcaaga acggctacgc cggctacatt gacggcggag ccagccagga agagttctac
     4801 aagttcatca agcccatcct ggaaaagatg gacggcaccg aggaactgct cgtgaagctg
     4861 aacagagagg acctgctgcg gaagcagcgg accttcgaca acggcagcat cccccaccag
     4921 atccacctgg gagagctgca cgccattctg cggcggcagg aagattttta cccattcctg
     4981 aaggacaacc gggaaaagat cgagaagatc ctgaccttcc gcatccccta ctacgtgggc
     5041 cctctggcca ggggaaacag cagattcgcc tggatgacca gaaagagcga ggaaaccatc
     5101 accccctgga acttcgagga agtggtggac aagggcgctt ccgcccagag cttcatcgag
     5161 cggatgacca acttcgataa gaacctgccc aacgagaagg tgctgcccaa gcacagcctg
     5221 ctgtacgagt acttcaccgt gtataacgag ctgaccaaag tgaaatacgt gaccgaggga
     5281 atgagaaagc ccgccttcct gagcggcgag cagaaaaagg ccatcgtgga cctgctgttc
     5341 aagaccaacc ggaaagtgac cgtgaagcag ctgaaagagg actacttcaa gaaaatcgag
     5401 tgcttcgact ccgtggaaat ctccggcgtg gaagatcggt tcaacgcctc cctgggcaca
     5461 taccacgatc tgctgaaaat tatcaaggac aaggacttcc tggacaatga ggaaaacgag
     5521 gacattctgg aagatatcgt gctgaccctg acactgtttg aggacagaga gatgatcgag
     5581 gaacggctga aaacctatgc ccacctgttc gacgacaaag tgatgaagca gctgaagcgg
     5641 cggagataca ccggctgggg caggctgagc cggaagctga tcaacggcat ccgggacaag
     5701 cagtccggca agacaatcct ggatttcctg aagtccgacg gcttcgccaa cagaaacttc
     5761 atgcagctga tccacgacga cagcctgacc tttaaagagg acatccagaa agcccaggtg
     5821 tccggccagg gcgatagcct gcacgagcac attgccaatc tggccggcag ccccgccatt
     5881 aagaagggca tcctgcagac agtgaaggtg gtggacgagc tcgtgaaagt gatgggccgg
     5941 cacaagcccg agaacatcgt gatcgaaatg gccagagaga accagaccac ccagaaggga
     6001 cagaagaaca gccgcgagag aatgaagcgg atcgaagagg gcatcaaaga gctgggcagc
     6061 cagatcctga aagaacaccc cgtggaaaac acccagctgc agaacgagaa gctgtacctg
     6121 tactacctgc agaatgggcg ggatatgtac gtggaccagg aactggacat caaccggctg
     6181 tccgactacg atgtggacca catcgtgcct cagagctttc tgaaggacga ctccatcgac
     6241 aacaaggtgc tgaccagaag cgacaaggcc cggggcaaga gcgacaacgt gccctccgaa
     6301 gaggtcgtga agaagatgaa gaactactgg cggcagctgc tgaacgccaa gctgattacc
     6361 cagagaaagt tcgacaatct gaccaaggcc gagagaggcg gcctgagcga actggataag
     6421 gccggcttca tcaagagaca gctggtggaa acccggcaga tcacaaagca cgtggcacag
     6481 atcctggact cccggatgaa cactaagtac gacgagaatg acaagctgat ccgggaagtg
     6541 aaagtgatca ccctgaagtc caagctggtg tccgatttcc ggaaggattt ccagttttac
     6601 aaagtgcgcg agatcaacaa ctaccaccac gcccacgacg cctacctgaa cgccgtcgtg
     6661 ggaaccgccc tgatcaaaaa gtaccctaag ctggaaagcg agttcgtgta cggcgactac
     6721 aaggtgtacg acgtgcggaa gatgatcgcc aagagcgagc aggaaatcgg caaggctacc
     6781 gccaagtact tcttctacag caacatcatg aactttttca agaccgagat taccctggcc
     6841 aacggcgaga tccggaagcg gcctctgatc gagacaaacg gcgaaaccgg ggagatcgtg
     6901 tgggataagg gccgggattt tgccaccgtg cggaaagtgc tgagcatgcc ccaagtgaat
     6961 atcgtgaaaa agaccgaggt gcagacaggc ggcttcagca aagagtctat cctgcccaag
     7021 aggaacagcg ataagctgat cgccagaaag aaggactggg accctaagaa gtacggcggc
     7081 ttcgacagcc ccaccgtggc ctattctgtg ctggtggtgg ccaaagtgga aaagggcaag
     7141 tccaagaaac tgaagagtgt gaaagagctg ctggggatca ccatcatgga aagaagcagc
     7201 ttcgagaaga atcccatcga ctttctggaa gccaagggct acaaagaagt gaaaaaggac
     7261 ctgatcatca agctgcctaa gtactccctg ttcgagctgg aaaacggccg gaagagaatg
     7321 ctggcctctg ccggcgaact gcagaaggga aacgaactgg ccctgccctc caaatatgtg
     7381 aacttcctgt acctggccag ccactatgag aagctgaagg gctcccccga ggataatgag
     7441 cagaaacagc tgtttgtgga acagcacaag cactacctgg acgagatcat cgagcagatc
     7501 agcgagttct ccaagagagt gatcctggcc gacgctaatc tggacaaagt gctgtccgcc
     7561 tacaacaagc accgggataa gcccatcaga gagcaggccg agaatatcat ccacctgttt
     7621 accctgacca atctgggagc ccctgccgcc ttcaagtact ttgacaccac catcgaccgg
     7681 aagaggtaca ccagcaccaa agaggtgctg gacgccaccc tgatccacca gagcatcacc
     7741 ggcctgtacg agacacggat cgacctgtct cagctgggag gcgacagcgc tggaggaggt
     7801 ggaagcggag gaggaggaag cggaggagga ggtagcggac ctaagaaaaa gaggaaggtg
     7861 gcggccgctg gatccggacg ggctgacgca ttggacgatt ttgatctgga tatgctggga
     7921 agtgacgccc tcgatgattt tgaccttgac atgcttggtt cggatgccct tgatgacttt
     7981 gacctcgaca tgctcggcag tgacgccctt gatgatttcg acctggacat gctgattaac
     8041 tgtacaggca gtggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
     8101 cctggcccaa tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     8161 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     8221 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     8281 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     8341 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     8401 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     8461 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaga attcgatatc
     8521 aagcttatcg gtaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     8581 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     8641 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     8701 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     8761 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     8821 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     8881 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     8941 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     9001 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     9061 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     9121 catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag tagcaataca
     9181 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
     9241 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
     9301 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
     9361 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
     9421 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     9481 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
     9541 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
     9601 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
     9661 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9721 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     9781 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac
     9841 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
     9901 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
     9961 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga
    10021 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
    10081 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag
    10141 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
    10201 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
    10261 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
    10321 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
    10381 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
    10441 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
    10501 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt
    10561 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
    10621 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
    10681 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
    10741 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
    10801 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
    10861 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata
    10921 tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag tatatcggca
    10981 tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt gccgttccgg
    11041 tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg ctcgggttct
    11101 cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg accctgttca
    11161 tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg
    11221 gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct
    11281 ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc gccctgcgcg
    11341 acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac gtgctacgag
    11401 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg
    11461 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact
    11521 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
    11581 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
    11641 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc
    11701 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
    11761 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc
    11821 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
    11881 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct
    11941 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
    12001 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
    12061 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
    12121 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
    12181 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
    12241 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
    12301 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
    12361 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
    12421 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
    12481 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
    12541 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
    12601 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
    12661 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
    12721 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
    12781 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
    12841 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
    12901 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
    12961 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
    13021 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
    13081 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
    13141 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
    13201 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
    13261 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
    13321 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
    13381 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
    13441 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
    13501 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
    13561 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
    13621 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
    13681 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
    13741 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
    13801 taggggttcc gcg
//
