LOCUS       sequence-170208-         774 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express sgRNA target HUWE1 gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Xu Y, Anderson DE, Ye Y
  TITLE     The HECT domain ubiquitin ligase HUWE1 targets unassembled soluble 
            proteins for degradation.
  JOURNAL   Cell Discov. 2016 Nov 8;2:16040. eCollection 2016.
  PUBMED    27867533
REFERENCE   2  (bases 1 to 774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2016-11-8"; volume: "2"; pages: "16040. eCollection
            2016"
COMMENT     Sequence Label: HUWE1-sgRNA-2
FEATURES             Location/Qualifiers
     source          1..774
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     96..115
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        194..269
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        366..651
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 tnganctnta ccacaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg
       61 ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt
      121 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac accgaggctg
      181 ttgatcgcgc ggcgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
      241 aacttgaaaa agtggcaccg agtcggtgct tttttgtttt agagctagaa atagcaagtt
      301 aaaataaggc tagtccgttt ttagcgcgtg cgccaattct gcagacaaat ggctctagag
      361 gtacccgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
      421 ccattgacgt caatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      481 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      541 attgacgtca atgacggtaa atggcccgcc tggcattgtg cccagtacat gaccttatgg
      601 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtcgaggtg
      661 agccccacgt tctgcttcac tctccccatc tcccccccct ccccaccccc aattttgtat
      721 ttatttattt tttaattatt ttgtgcagcg atgggggcgg gggggggggg gggg
//
