LOCUS       sequence-17022-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Stewart SA, Dykxhoorn DM, Palliser D, Mizuno H, Yu EY, An DS, 
            Sabatini DM, Chen IS, Hahn WC, Sharp PA, Weinberg RA, Novina CD
  TITLE     Lentivirus-delivered stable gene silencing by RNAi in primary cells.
  JOURNAL   RNA 2003 Apr;9(4):493-501.
  PUBMED    12649500
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "RNA"; date:
            "2003-04"; volume: "9(4)"; pages: "493-501"
COMMENT     Sequence Label: Retrohair
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        46..294
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     46..66
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     217..236
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(369..389)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        374..703
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      554..689
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 tccttctctg gcgccggccg gatctgtcga caggtcgggc aggaagaggg cctatttccc
       61 atgattcctt catatttgca tatacgatac aaggctgtta gagagataat tagaattaat
      121 ttgactgtaa acacaaagat attagtacaa aatacgtgac gtagaaagta ataatttctt
      181 gggtagtttg cagttttaaa attatgtttt aaaatggact atcatatgct taccgtaact
      241 tgaaagtatt tcgatttctt ggctttatat atcttgtgga aaggacgaaa caccgggccc
      301 tctagactcg agcggccgcc actgtgctgg atatctgcag aattcgccag cacagtggtc
      361 gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
      421 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
      481 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
      541 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
      601 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
      661 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatga
//
