LOCUS       sequence-170223-        9620 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  lentiviral expression of Clover.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9620)
  TITLE     See lab plasmids
REFERENCE   2  (bases 1 to 9620)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLNT-SFFV-Clover
FEATURES             Location/Qualifiers
     source          1..9620
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(9..11,12..14,15..725)
                     /codon_start=1
                     /product="bright green-yellow fluorescent protein derived 
                     from GFP (Lam et al., 2012)"
                     /label=Clover
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGVACFSRYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK
                     ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSALSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     primer_bind     complement(54..75)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     662..683
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    766..1354
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(819..839)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1237..1248)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(1357..1373)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1358..1374)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             1464..1697
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(1722..1740)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1723..1740)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(2030..2049)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2263..2282)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     2382..2404
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2442..2460)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2528..2632
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2633..2701,2702..3493)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2851..2870)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3664..4252
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4153..4172
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4406..4423
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(4493..4513)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4498..4827
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4678..4813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          5961..6026
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             6156..6176
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    6601..6735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6638..6657)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6692..6711
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6904..7537
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7584..7709
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8202..8435
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8620..8664
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8949..9066
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        9219..9620
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
ORIGIN
        1 gatccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg
       61 agctggacgg cgacgtaaac ggccacaagt tcagcgtccg cggcgagggc gagggcgatg
      121 ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct
      181 ggcccaccct cgtgaccacc ttcggctacg gcgtggcctg cttcagccgc taccccgacc
      241 acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca
      301 ccatctcttt caaggacgac ggtacctaca agacccgcgc cgaggtgaag ttcgagggcg
      361 acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc
      421 tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcacg gccgacaagc
      481 agaagaacgg catcaaggct aacttcaaga tccgccacaa cgttgaggac ggcagcgtgc
      541 agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg
      601 acaaccacta cctgagccat cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc
      661 acatggtcct gctggagttc gtgaccgccg ccgggattac acatggcatg gacgagctgt
      721 acaagtaacc tgcaggcatg caagcttgat atcaagctta tcgataatca acctctggat
      781 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
      841 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
      901 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
      961 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     1021 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     1081 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     1141 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     1201 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     1261 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     1321 acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat
     1381 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     1441 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     1501 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     1561 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     1621 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     1681 gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg tcacctaaat
     1741 cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa cgacaatatg
     1801 tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc tctctcgtaa
     1861 actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa cgccgtcccg
     1921 cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat cctctacgcc
     1981 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc
     2041 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc
     2101 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca
     2161 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg
     2221 caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct gctctgatgc
     2281 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     2341 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     2401 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     2461 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     2521 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     2581 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     2641 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     2701 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     2761 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     2821 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     2881 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     2941 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3001 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3061 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     3121 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     3181 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     3241 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     3301 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     3361 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     3421 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     3481 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     3541 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     3601 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     3661 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     3721 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     3781 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     3841 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     3901 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     3961 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4021 gacctacacc gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga
     4081 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     4141 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     4201 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     4261 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc
     4321 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
     4381 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc
     4441 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct gtggaatgtg
     4501 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4561 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4621 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4681 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4741 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     4801 ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt gcgagatatg
     4861 tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc
     4921 atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac ctattttccc
     4981 ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga gcgaaaaata
     5041 catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc
     5101 ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta
     5161 ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag ctacgatcac
     5221 gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc
     5281 gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga aatgtatcca
     5341 aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt tgatgactat
     5401 gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg cgtcgtattc
     5461 gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg cgttgttctt
     5521 tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg catccatgac
     5581 acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg aaacctcgac
     5641 gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc ttgatggtaa
     5701 aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc gcggcattga
     5761 cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg aacgtaccga
     5821 cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt atgagtgggc
     5881 cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac aaactaccta
     5941 cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt gttaaactac
     6001 tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa tgggagcagt
     6061 ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca tctagtgatg
     6121 atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga aaggtagaag
     6181 accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg tttagtaata
     6241 gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg ctatacaaga
     6301 aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat aatcataaca
     6361 tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac tatgctcaaa
     6421 aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat ttgatgtata
     6481 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     6541 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     6601 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     6661 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     6721 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
     6781 ccatacccta ttaccactgc caattaccta gtggtttcat ttactctaaa cctgtgattc
     6841 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     6901 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     6961 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     7021 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     7081 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     7141 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     7201 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     7261 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     7321 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     7381 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7441 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7501 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     7561 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     7621 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     7681 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     7741 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     7801 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     7861 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     7921 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     7981 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     8041 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     8101 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     8161 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     8221 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     8281 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     8341 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     8401 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     8461 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     8521 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     8581 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     8641 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     8701 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     8761 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     8821 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     8881 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
     8941 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     9001 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     9061 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
     9121 gaattcctgc agccccgata aaataaaaga ttttatttag tctccagaaa aaggggggaa
     9181 tgaaagaccc cacctgtagg tttggcaagc tagctgcagt aacgccattt tgcaaggcat
     9241 ggaaaaatac caaaccaaga atagagaagt tcagatcaag ggcgggtaca tgaaaatagc
     9301 taacgttggg ccaaacagga tatctgcggt gagcagtttc ggccccggcc cggggccaag
     9361 aacagatggt caccgcagtt tcggccccgg cccgaggcca agaacagatg gtccccagat
     9421 atggcccaac cctcagcagt ttcttaagac ccatcagatg tttccaggct cccccaagga
     9481 cctgaaatga ccctgcgcct tatttgaatt aaccaatcag cctgcttctc gcttctgttc
     9541 gcgcgcttct gcttcccgag ctctataaaa gagctcacaa cccctcactc ggcgcgccag
     9601 tcctccgaca gactgagtcg
//
