LOCUS       sequence-170245-        1900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gtbp‐1 (~500 bp flanking sequence)::GFP (with 3 introns)::gtbp‐1 
            (~500 bp flanking sequence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1900)
  AUTHORS   Paix A, Schmidt H, Seydoux G
  TITLE     Cas9-assisted recombineering in C. elegans: genome editing using in 
            vivo assembly of linear DNAs.
  JOURNAL   Nucleic Acids Res. 2016 Sep 6;44(15):e128. doi: 10.1093/nar/gkw502. 
            Epub 2016 Jun 1.
  PUBMED    27257074
REFERENCE   2  (bases 1 to 1900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2016-09-6"; volume: "44(15):e128. doi"; pages: "
            10.1093/nar/gkw502. Epub 2016 Jun 1"
COMMENT     Sequence Label: AP553‐1
FEATURES             Location/Qualifiers
     source          1..1900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(547..575)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1323..1343
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 cggtacccgg ggatcgcgcc aagctcagga aagttcagat gccaacctaa atgagcgtaa
       61 gatctatttg ggaggaatca ctcgagagat tgtccccagc aacacagctc aggctgaatc
      121 ggaaatcaag caggtgttct acagtaagca aattaatagt cccaatagtt cactaaattt
      181 tcattttaag aatatggaga aatcgaggag gttcgcatgc cgcgcaaggt tcttgacagt
      241 ccgaacgaca catcgaaaca tgcctttgca tttattacca tgcgtaacaa ggccggagta
      301 aaagccattt tcgaagcggc tacaaaggat gagcaaggcg tgtggaagct tccactcaaa
      361 ctggatgcat tcggatttga cggacatgct caaattagtg agcaacgtga tcaaccacaa
      421 ggcggcggat tccgaggagg acgtggaggt ttccaaccac gcggtggaca attcttccgc
      481 cgtggcggtt cgggtggtgc tccacgaggt ggtatgcgca gtaaaggaga agaacttttc
      541 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      601 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      661 actactggaa aactacctgt tccatgggta agtttaaaca tatatatact aactaaccct
      721 gattatttaa attttcagcc aacacttgtc actactttct gttatggtgt tcaatgcttc
      781 tcgagatacc cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      841 tatgtacagg aaagaactat atttttcaaa gatgacggga actacaagac acgtaagttt
      901 aaacagttcg gtactaacta accatacata tttaaatttt caggtgctga agtcaagttt
      961 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     1021 aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     1081 gacaaacaaa agaatggaat caaagttgta agtttaaaca tgattttact aactaactaa
     1141 tctgatttaa attttcagaa cttcaaaatt agacacaaca ttgaagatgg aagcgttcaa
     1201 ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac
     1261 aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa gagagaccac
     1321 atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga cgaactatac
     1381 aaaagcggtt tccaaaatgc gggacaaaat tagaagcttt ccgttctcct ttttccttct
     1441 tgtaagaatt gcacatccat tagattctgt acctatcctt ctcattttcc tatttattgt
     1501 ttctcctttc cttcctcacc gttctaataa tgatgattta agtaagaaga ttctggtatc
     1561 tgaattttat ggcgtgtagc gttttagtag atctctcaac aatccaatcc aatcagcccg
     1621 catcccattt tcacatccta tctccccggt tttcggtgat tgacgaccgt gatcccatct
     1681 gagtttactc ttttcgagta caagttgcaa tcagacatca tcccaccacc gaatatccaa
     1741 gttgtatttg aatgttgttt taatatatta ccactttcct tctcctttta caagcccccg
     1801 ttattgtttc cttcattggt gttcgggagt actctttgta ctcaagttct tacccccatt
     1861 ttggcacgtt ggaattaaaa taaaggatcc tctagagtcg
//
