LOCUS       sequence-170253-       10214 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses ParB1-mCherry in yeast. Also known as pOR1-mch.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10214)
  AUTHORS   Saad H, Gallardo F, Dalvai M, Tanguy-le-Gac N, Lane D, Bystricky K
  TITLE     DNA dynamics during early double-strand break processing revealed by
            non-intrusive imaging of living cells.
  JOURNAL   PLoS Genet. 2014 Mar 13;10(3):e1004187. doi: 
            10.1371/journal.pgen.1004187. eCollection 2014 Mar.
  PUBMED    24625580
REFERENCE   2  (bases 1 to 10214)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Genet."; date: "2014-03-13"; volume: "10(3):e1004187. doi"; pages: "
            10.1371/journal.pgen.1004187. eCollection 2014 Mar"
COMMENT     Sequence Label: pCM189-ParB1::mCherry
FEATURES             Location/Qualifiers
     source          1..10214
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(28..414,415..1035))
                     /codon_start=1
                     /product="tetracycline-controlled transactivator, 
                     comprising a fusion of the tetracycline repressor TetR with
                     the C-terminal activation domain of herpes simplex virus 
                     VP16"
                     /label=tTA
                     /note="In the Tet-Off(R) system, tTA binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the absence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGSAYSRARTKNNYGSTI
                     EGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSLGDELHLDGEDVAMAHA
                     DALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     943..962
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     primer_bind     complement(1120..1144)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        complement(1141..1344)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1170..1190)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1345..1724
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     terminator      1836..2023
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(1891..1915)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     protein_bind    join(2081..2099,2100..2122,2123..2141,2142..2164,2165..2183,
                     2184..2206,2207..2225,2226..2248,2249..2267,2268..2290,
                     2291..2309,2310..2332,2333..2351)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     CDS             3676..4386
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3821..3839)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4089..4108
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4418..4665
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(4434..4452)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(4688..4704)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4688..4704)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4701..4723)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4712..4728
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4736..4742,4743..4760,4761..4766))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4781..4802
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4919..4936)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5090..5678)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5170..5189)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5849..6640,6641..6709))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6472..6491
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6710..6814)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6882..6900
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6925..7140
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     CDS             7141..7944
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    8279..9441
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the 
                     autonomously replicating sequence ARS1/ARS416"
     rep_origin      8279..9116
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence 
                     ARS1/ARS416"
     primer_bind     10183..10205
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     10197..10214
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     10198..10214
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gaattcttat tacgatcctc gcgcccccta cccaccgtac tcgtcaattc caagggcatc
       61 ggtaaacatc tgctcaaact cgaagtcggc catatccaga gcgccgtagg gggcggagtc
      121 gtggggggta aatcccggac ccggggaatc cccgtccccc aacatgtcca gatcgaaatc
      181 gtctagcgcg tcggcatgcg ccatcgccac gtcctcgccg tctaagtgga gctcgtcccc
      241 caggctgaca tcggtcgggg gggccgtcga cagtctgcgc gtgtgtcccg cggggagaaa
      301 ggacaggcgc ggagccgcca gccccgcctc ttcgggggcg tcgtcgtccg ggagatcgag
      361 caggccctcg atggtagacc cgtaattgtt tttcgtacgc gcgcggctgt acgcggaccc
      421 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      481 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      541 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      601 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      661 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      721 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      781 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      841 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      901 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      961 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
     1021 atctaatcta gacatatgaa ttaattcggg gccgcggagg ctggatcggt cccggtgtct
     1081 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
     1141 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     1201 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     1261 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     1321 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     1381 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     1441 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     1501 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     1561 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     1621 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     1681 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacatg gcggtaatgt
     1741 tggacatgag ccaatataaa tgtacatatt atgatatgga tacaacgtat gcaatgggcc
     1801 aagctcctcg agtaattcgc gccacttcta aataagcgaa tttcttatga tttatgattt
     1861 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
     1921 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
     1981 aggtatagta tgaggtcgct cttattgacc acacctctac cggcagatcc gctagggata
     2041 acagggtaat atagatcaat tcctcgatcg agtttaccac tccctatcag tgatagagaa
     2101 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     2161 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     2221 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     2281 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     2341 agtgatagag aaaagtgaaa gtcgagctcg gtaccctatg gcatgcatgt gctctgtatg
     2401 tatataaaac tcttgttttc ttcttttctc taaatattct ttccttatac attaggtcct
     2461 ttgtagcata aattactata cttctataga cacgcaaaca caaatacaca cactaaatta
     2521 ccggatcaat tcgggggatc ctacctgacg ctttttatcg caactctcta ctgtttctcc
     2581 atacccgttt ttttgggcta gcaggaggaa ttgatgaaac cctcccaatt tgccaaagga
     2641 ttccaagcgc gcccggatat cacgacgagc gagaagcgca cggcgcttga tcggctcaat
     2701 gcgatcgacg gcatcgtcaa gtccgagacg ccgaccccgg cgccgaccaa atccgcgaag
     2761 aaggacatcg caccgccgcc tgctccggaa ttcacgatcg atccgtcgat cgacgaatcg
     2821 cagcaatatc gcgcgtggcg tctcgagaat cgctatgcac ccgggcaggt gatcgagctg
     2881 ccgctgaagg cgatcaagca cagcccgttc aacccgcggc acttctatct gaaatcgtcg
     2941 attgccgaac tcgcggtcaa cctcgcgaag cagggacagc agcaggcgat ccacgtgatt
     3001 ccggattacg acaacccggg cacgtatttc gtcagcgatg gcgggcgtcg cgtgcgggcg
     3061 ctgaaggaag cgaacaagga atcggtcaag gcgatcgtga tcgatgtgcc gatcggcatc
     3121 cagagctaca agctcggcta cgacctgaac gttcagcgcg attcgcagac ggtgttcgac
     3181 aacgccgtcg tgtggcgccg cttcctcgac gacaagcatt tccagagcca gaaagaactc
     3241 tccgagcatc tcggcctcga cgagtcgacg gtggccgtcg cgctgtcgat cggcaagttg
     3301 ccggaagcga tcatgcagga aatggtcgca cgccccgatc gcttcggatc gaacatggcg
     3361 tatcaggtcg gccgctatca caatgcgcgc ggcaccgagg ccacgctgcg gctgatcaac
     3421 aagatcgtgt cggacgatct cagcacgcgc caggtgtcgg acatcgtcaa gggtcgcgtc
     3481 gcggcacagg agacgccgaa gcctgcgggc cgtcagcgct atgcgcagcg tctcgagatc
     3541 aagctcggcg gcaagtcggt cggcgacctg aagtcgtatg gcgaagaccg catcgaattg
     3601 cgcctgcgcg gcctcccgaa ggacaagcgc gacgcgattc tcgagcagct cgagcggatg
     3661 ctgttgtcgg agcatatggt gagcaagggc gaggaggata acatggccat catcaaggag
     3721 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag
     3781 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag
     3841 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag
     3901 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc
     3961 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac
     4021 tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc
     4081 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg
     4141 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc
     4201 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc
     4261 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc
     4321 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac
     4381 aagtaagcgg ccgctagggc cctgcaggag ggccgcatca tgtaattagt tatgtcacgc
     4441 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     4501 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     4561 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     4621 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggcca agcttggcgt
     4681 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4741 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4801 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4861 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4921 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4981 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     5041 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     5101 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     5161 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     5221 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     5281 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     5341 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     5401 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     5461 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     5521 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     5581 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5641 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5701 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5761 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5821 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5881 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5941 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     6001 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     6061 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     6121 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     6181 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     6241 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     6301 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     6361 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     6421 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     6481 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     6541 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6601 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6661 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6721 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6781 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6841 acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     6901 cctttcgtct tcaagaatta gcttttcaat tcatcatttt ttttttattc ttttttttga
     6961 tttcggtttc cttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg
     7021 aaggagcaca gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat
     7081 tgcccagtat tcttaaccca actgcacaga acaaaaacat gcaggaaacg aagataaatc
     7141 atgtcgaaag ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag
     7201 ctatttaata tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc
     7261 accaaggaat tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca
     7321 catgtggata tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta
     7381 tccgccaagt acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca
     7441 gtcaaattgc agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat
     7501 gcacacggtg tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta
     7561 acaaaggaac ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct
     7621 actggagaat atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc
     7681 ggctttattg ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg
     7741 acacccggtg tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg
     7801 gatgatgtgg tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag
     7861 ggaagggatg ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg
     7921 agaagatgcg gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac
     7981 tcacaaatta gagcttcaat ttaattatat cagttattac ccaattctca tgtttgacag
     8041 cttatcatcg atcgtccaac tgcatggaga tgagtcgtgg caagaatacc aagagttcct
     8101 cggtttgcca gttattaaaa gactcgtatt tccaaaagac tgcaacatac tactcagtgc
     8161 agcttcacag aaacctcatt cgtttattcc cttgtttgat tcagaagcag gtgggacagg
     8221 tgaacttttg gattggaact cgatttctga ctgggttgga aggcaagaga gccccgagag
     8281 cttacatttt atgttagctg gtggactgac gccagaaaat gttggtgatg cgcttagatt
     8341 aaatggcgtt attggtgttg atgtaagcgg aggtgtggag acaaatggtg taaaagactc
     8401 taacaaaata gcaaatttcg tcaaaaatgc taagaaatag gttattactg agtagtattt
     8461 atttaagtat tgtttgtgca cttgcctgca agccttttga aaagcaagca taaaagatct
     8521 aaacataaaa tctgtaaaat aacaagatgt aaagataatg ctaaatcatt tggctttttg
     8581 attgattgta caggaaaata tacatcgcag ggggttgact tttaccattt caccgcaatg
     8641 gaatcaaact tgttgaagag aatgttcaca ggcgcatacg ctacaatgac ccgattcttg
     8701 ctagcctttt ctcggtcttg caaacaaccg ccggcagctt agtatataaa tacacatgta
     8761 catacctctc tccgtatcct cgtaatcatt ttcttgtatt tatcgtcttt tcgctgtaaa
     8821 aactttatca cacttatctc aaatacactt attaaccgct tttactatta tcttctacgc
     8881 tgacagtaat atcaaacagt gacacatatt aaacacagtg gtttctttgc ataaacacca
     8941 tcagcctcaa gtcgtcaagt aaagatttcg tgttcatgca gatagataac aatctatatg
     9001 ttgataatta gcgttgcctc atcaatgcga gatccgttta accggaccct agtgcactta
     9061 ccccacgttc ggtccactgt gtgccgaaca tgctccttca ctattttaac atgtggaatt
     9121 aattctcatg tttgacagct tatcatcgaa ctctaagagg tgatacttat ttactgtaaa
     9181 actgtgacga taaaaccgga aggaagaata agaaaactcg aactgatcta taatgcctat
     9241 tttctgtaaa gagtttaagc tatgaaagcc tcggcatttt ggccgctcct aggtagtgct
     9301 ttttttccaa ggacaaaaca gtttcttttt cttgagcagg ttttatgttt cggtaatcat
     9361 aaacaataaa taaattattt catttatgtt taaaaataaa aaataaaaaa gtattttaaa
     9421 tttttaaaaa agttgattat aagcatgtga ccttttgcaa gcaattaaat tttgcaattt
     9481 gtgatttagg caaaagttac tatttctggc tcgtgtaata tatgtatgct aatgtgaact
     9541 tttacaaagt cgatatggac ttagtcaaaa gaaattttct taaaaatata tagcactagc
     9601 caatttagca cttctttatg agatatatta tagactttat taagccagat ttgtgtatta
     9661 tatgtattta cccggcgaat catggacata cattctgaaa taggtaatat tctctatggt
     9721 gagacagcat agataaccta ggatacaagt taaaagctag tactgttttg cagtaatttt
     9781 tttctttttt ataagaatgt taccacctaa ataagttata aagtcaatag ttaagtttga
     9841 tatttgattg taaaataccg taatatattt gcatgatcaa aaggctcaat gttgactagc
     9901 cagcatgtca accactatat tgatcaccga tattaggact tccacaccaa ctagtaatat
     9961 gacaataaat tcaagatatt cttcatgaga atggcccagc gattatgcgg tgtgaaatac
    10021 cgcacagatg cgtaaggaga aaataccgca tcaggcgcca ttcgccattc aggctgcgca
    10081 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
    10141 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
    10201 aaacgacggc cagt
//
