LOCUS       sequence-170271-         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Scheffner M, Huibregtse JM, Howley PM
  TITLE     Identification of a human ubiquitin-conjugating enzyme that mediates
            the E6-AP-dependent ubiquitination of p53.
  JOURNAL   Proc Natl Acad Sci U S A 1994 Sep 13;91(19):8797-801.
  PUBMED    8090726
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1994-09-13"; volume: "91(19)"; pages: 
            "8797-801"
COMMENT     Sequence Label: p3993 pET ubcH7
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..20
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1..19
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             51..73
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      complement(546..593)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
       61 tttaagaagg agatatacat atggcggcca gcaggaggct gatgaaggag cttgaagaaa
      121 tccgcaaatg tgggatgaaa aacttccgta acatccaggt tgatgaagct aatttattga
      181 cttggcaagg gcttattgtt cctgacaacc ctccatatga taagggagcc ttcagaatcg
      241 aaatcaactt tccagcagag tacccattca aaccaccgaa gatcacattt aaaacaaaga
      301 tctatcaccc aaacatcgac gaaaaggggc aggtctgtct gccagtaatt agtgccgaaa
      361 actggaagcc agcaaccaaa accgaccaag taatccagtc cctcatagca ctggtgaatg
      421 acccccagcc tgagcacccg cttcgggctg acctagctga agaatactct aaggaccgta
      481 aaaaattctg taagaatgct gaagagttta caaagaaata tggggaaaag cgacctgtgg
      541 actaacaaaa aacccctcaa gacccgttta gaggccccaa ggggttatgc tag
//
