LOCUS       sequence-170274-        9471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses S.aureus FokI-dCas9-NLS with a 36 amino acid GSAT linker 
            fusion. Also expresses Csy4 for cleavage of multiplexed gRNA 
            transcripts. Backbone derived from pSQT1601 (Addgene #53369)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9471)
  AUTHORS   Havlicek S, Shen Y, Alpagu Y, Bruntraeger MB, Zufir NB, Phuah ZY, Fu
            Z, Dunn NR, Stanton LW
  TITLE     Re-engineered RNA-Guided FokI-Nucleases for Improved Genome Editing 
            in Human Cells.
  JOURNAL   Mol Ther. 2017 Feb 1;25(2):342-355. doi: 
            10.1016/j.ymthe.2016.11.007.
  PUBMED    28153087
REFERENCE   2  (bases 1 to 9471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ymthe.2016.11"; journalName: "Mol Ther"; date: 
            "2017-02-1- 1"; volume: "25"; issue: "2"; pages: "342-355"
COMMENT     Sequence Label: pSH-Csy4-T2A-SaRFN
FEATURES             Location/Qualifiers
     source          1..9471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          166..1174
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1098..1120
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1182..1201
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1267..1276
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1837..1890
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1894..1914
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1921..2508
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     CDS             5773..5820
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             5827..5907
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     primer_bind     complement(5997..6016)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    6062..6117
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(6116..6135)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6478..6494)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6478..6494)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6491..6513)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6502..6518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6526..6532,6533..6550,6551..6556))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6571..6592
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        6651..6846
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6697..6832
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    6852..6986
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6889..6908)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6943..6962
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7054..7071)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7225..7813)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7305..7324)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7984..8775,8776..8844))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8607..8626
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8845..8949)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        8979..9358
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        join(9361..9471,1..165)
                     /label=chicken beta-actin promoter
ORIGIN
        1 gggggggggc gcgcgccagg cggggcgggg cggggcgagg ggcggggcgg ggcgaggcgg
       61 agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa agtttccttt tatggcgagg
      121 cggcggcggc ggcggcccta taaaaagcga agcgcgcggc gggcgggagt cgctgcgttg
      181 ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc cgccccggct ctgactgacc
      241 gcgttactcc cacaggtgag cgggcgggac ggcccttctc ctccgggctg taattagcgc
      301 ttggtttaat gacggctcgt ttcttttctg tggctgcgtg aaagccttaa agggctccgg
      361 gagggccctt tgtgcggggg ggagcggctc ggggggtgcg tgcgtgtgtg tgtgcgtggg
      421 gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc gctgcgggcg cggcgcgggg
      481 ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg gggcggtgcc ccgcggtgcg
      541 ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg ggtgagcagg
      601 gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca cccccctccc cgagttgctg
      661 agcacggccc ggcttcgggt gcggggctcc gtgcggggcg tggcgcgggg ctcgccgtgc
      721 cgggcggggg gtggcggcag gtgggggtgc cgggcggggc ggggccgcct cgggccgggg
      781 agggctcggg ggaggggcgc ggcggccccg gagcgccggc ggctgtcgag gcgcggcgag
      841 ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc ctttgtccca
      901 aatctggcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcgggcga
      961 agcggtgcgg cgccggcagg aaggaaatgg gcggggaggg ccttcgtgcg tcgccgcgcc
     1021 gccgtcccct tctccatctc cagcctcggg gctgccgcag ggggacggct gccttcgggg
     1081 gggacggggc agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc
     1141 taaccatgtt catgccttct tctttttcct acagctcctg ggcaacgtgc tggttattgt
     1201 gctgtctcat cattttggca aagaattctg cagtcgacgg taccgcgggc ccgggatcca
     1261 ccggtcgcca ccatgggtga tcattatctg gatattcggc tgaggcctga tccagagttc
     1321 ccacctgcgc agctgatgtc tgtccttttt ggcaaacttc atcaggccct ggttgcccag
     1381 ggcggagatc ggataggggt aagctttcca gacctcgacg aaagccggag ccgcctggga
     1441 gaacgcctgc ggatccacgc ttctgccgac gatctgagag ccttgctggc aaggccatgg
     1501 cttgaggggc tccgggatca cctgcagttt ggcgaacccg ccgttgttcc ccacccaacc
     1561 ccttatcggc aggtgtctag agtgcaggcc aaatctaatc cagaacggct gcgacggcga
     1621 ctcatgcggc gacatgatct tagcgaggaa gaggcccgaa aaagaatccc tgataccgtg
     1681 gcccgcgccc ttgacttgcc ttttgtcaca ctgcggtccc agagtacggg gcagcatttc
     1741 agacttttca ttcgacacgg gccactgcaa gttaccgccg aagaaggagg ctttacttgt
     1801 tatggactct ccaagggagg tttcgtgccc tggtttgagg gcagaggaag tctgttaaca
     1861 tgcggtgacg tcgaggagaa tcctggccca atgcctaaga agaagcggaa ggtgagcagc
     1921 caacttgtga agtctgaact cgaggagaaa aaatcagagt tgagacacaa gttgaagtac
     1981 gtgccacacg aatacatcga gcttatcgag atcgccagaa acagtaccca ggataggatc
     2041 cttgagatga aagtcatgga gttctttatg aaggtctacg gttatagagg aaagcacctt
     2101 ggcggtagca gaaagcccga tggcgccatc tatactgtcg gatctcctat cgattatggg
     2161 gtgatcgtgg ataccaaagc ttactcaggc gggtacaact tgcccatagg acaagccgac
     2221 gagatgcagc ggtatgtcga agagaaccag acgcgcaaca agcacatcaa ccccaatgaa
     2281 tggtggaaag tgtacccaag tagtgtgact gagttcaagt tcctgtttgt ctccggccac
     2341 tttaagggca attataaagc tcagctcact agactcaatc acatcacaaa ctgcaacgga
     2401 gctgtgttgt cagtggagga gctcctgatt ggaggcgaga tgatcaaagc cggcaccctt
     2461 acactggagg aggtgcggcg gaagttcaac aatggagaga tcaacttcgg cggaagcgcc
     2521 ggaggcagtg gcagcgggag cagtggtgga agcagtggag ctagcggcac tggcacagcg
     2581 ggcggaactg ggagcggcag tggcacaggt agtggaaagc ggaactacat cctgggcctg
     2641 gccatcggca tcaccagcgt gggctacggc atcatcgact acgagacacg ggacgtgatc
     2701 gatgccggcg tgcggctgtt caaagaggcc aacgtggaaa acaacgaggg caggcggagc
     2761 aagagaggcg ccagaaggct gaagcggcgg aggcggcata gaatccagag agtgaagaag
     2821 ctgctgttcg actacaacct gctgaccgac cacagcgagc tgagcggcat caacccctac
     2881 gaggccagag tgaagggcct gagccagaag ctgagcgagg aagagttctc tgccgccctg
     2941 ctgcacctgg ccaagagaag aggcgtgcac aacgtgaacg aggtggaaga ggacaccggc
     3001 aacgagctgt ccaccaaaga gcagatcagc cggaacagca aggccctgga agagaaatac
     3061 gtggccgaac tgcagctgga acggctgaag aaagacggcg aagtgcgggg cagcatcaac
     3121 agattcaaga ccagcgacta cgtgaaagaa gccaaacagc tgctgaaggt gcagaaggcc
     3181 taccaccagc tggaccagag cttcatcgac acctacatcg acctgctgga aacccggcgg
     3241 acctactatg agggacctgg cgagggcagc cccttcggct ggaaggacat caaagaatgg
     3301 tacgagatgc tgatgggcca ctgcacctac ttccccgagg aactgcggag cgtgaagtac
     3361 gcctacaacg ccgacctgta caacgccctg aacgacctga acaatctcgt gatcaccagg
     3421 gacgagaacg agaagctgga atattacgag aagttccaga tcatcgagaa cgtgttcaag
     3481 cagaagaaga agcccaccct gaagcagatc gccaaagaaa tcctcgtgaa cgaagaggat
     3541 attaagggct acagagtgac cagcaccggc aagcccgagt tcaccaacct gaaggtgtac
     3601 cacgacatca aggacattac cgcccggaaa gagattattg agaacgccga gctgctggat
     3661 cagattgcca agatcctgac catctaccag agcagcgagg acatccagga agaactgacc
     3721 aatctgaact ccgagctgac ccaggaagag atcgagcaga tctctaatct gaagggctat
     3781 accggcaccc acaacctgag cctgaaggcc atcaacctga tcctggacga gctgtggcac
     3841 accaacgaca accagatcgc tatcttcaac cggctgaagc tggtgcccaa gaaggtggac
     3901 ctgtcccagc agaaagagat ccccaccacc ctggtggacg acttcatcct gagccccgtc
     3961 gtgaagagaa gcttcatcca gagcatcaaa gtgatcaacg ccatcatcaa gaagtacggc
     4021 ctgcccaacg acatcattat cgagctggcc cgcgagaaga actccaagga cgcccagaaa
     4081 atgatcaacg agatgcagaa gcggaaccgg cagaccaacg agcggatcga ggaaatcatc
     4141 cggaccaccg gcaaagagaa cgccaagtac ctgatcgaga agatcaagct gcacgacatg
     4201 caggaaggca agtgcctgta cagcctggaa gccatccctc tggaagatct gctgaacaac
     4261 cccttcaact atgaggtgga ccacatcatc cccagaagcg tgtccttcga caacagcttc
     4321 aacaacaagg tgctcgtgaa gcaggaagaa gccagcaaga agggcaaccg gaccccattc
     4381 cagtacctga gcagcagcga cagcaagatc agctacgaaa ccttcaagaa gcacatcctg
     4441 aatctggcca agggcaaggg cagaatcagc aagaccaaga aagagtatct gctggaagaa
     4501 cgggacatca acaggttctc cgtgcagaaa gacttcatca accggaacct ggtggatacc
     4561 agatacgcca ccagaggcct gatgaacctg ctgcggagct acttcagagt gaacaacctg
     4621 gacgtgaaag tgaagtccat caatggcggc ttcaccagct ttctgcggcg gaagtggaag
     4681 tttaagaaag agcggaacaa ggggtacaag caccacgccg aggacgccct gatcattgcc
     4741 aacgccgatt tcatcttcaa agagtggaag aaactggaca aggccaaaaa agtgatggaa
     4801 aaccagatgt tcgaggaaaa gcaggccgag agcatgcccg agatcgaaac cgagcaggag
     4861 tacaaagaga tcttcatcac cccccaccag atcaagcaca ttaaggactt caaggactac
     4921 aagtacagcc accgggtgga caagaagcct aatagagagc tgattaacga caccctgtac
     4981 tccacccgga aggacgacaa gggcaacacc ctgatcgtga acaatctgaa cggcctgtac
     5041 gacaaggaca atgacaagct gaaaaagctg atcaacaaga gccccgaaaa gctgctgatg
     5101 taccaccacg acccccagac ctaccagaaa ctgaagctga ttatggaaca gtacggcgac
     5161 gagaagaatc ccctgtacaa gtactacgag gaaaccggga actacctgac caagtactcc
     5221 aaaaaggaca acggccccgt gatcaagaag attaagtatt acggcaacaa actgaacgcc
     5281 catctggaca tcaccgacga ctaccccaac agcagaaaca aggtcgtgaa gctgtccctg
     5341 aagccctaca gattcgacgt gtacctggac aatggcgtgt acaagttcgt gaccgtgaag
     5401 aatctggatg tgatcaaaaa agaaaactac tacgaagtga atagcaagtg ctatgaggaa
     5461 gctaagaagc tgaagaagat cagcaaccag gccgagttta tcgcctcctt ctacaacaac
     5521 gatctgatca agatcaacgg cgagctgtat agagtgatcg gcgtgaacaa cgacctgctg
     5581 aaccggatcg aagtgaacat gatcgacatc acctaccgcg agtacctgga aaacatgaac
     5641 gacaagaggc cccccaggat cattaagaca atcgcctcca agacccagag cattaagaag
     5701 tacagcacag acattctggg caacctgtat gaagtgaaat ctaagaagca ccctcagatc
     5761 atcaaaaagg gcaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag
     5821 ggatcctacc catacgatgt tccagattac gcttacccat acgatgttcc agattacgct
     5881 tacccatacg atgttccaga ttacgcttaa agcggccgca ctcctcaggt gcaggctgcc
     5941 tatcagaagg tggtggctgg tgtggccaat gccctggctc acaaatacca ctgagatctt
     6001 tttccctctg ccaaaaatta tggggacatc atgaagcccc ttgagcatct gacttctggc
     6061 taataaagga aatttatttt cattgcaata gtgtgttgga attttttgtg tctctcactc
     6121 ggaaggacat atgggagggc aaatcattta aaacatcaga atgagtattt ggtttagagt
     6181 ttggcaacat atgcccatat gctggctgcc atgaacaaag gttggctata aagaggtcat
     6241 cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga
     6301 ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt
     6361 tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct
     6421 gtccctcttc tcttatggag atccctcgac ctgcagccca agcttggcgt aatcatggtc
     6481 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg
     6541 aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt
     6601 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat
     6661 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     6721 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     6781 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     6841 tgcaaaaagc taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6901 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6961 tcaatgtatc ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag
     7021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     7081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     7141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     7801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     7861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     7921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     7981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     8041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     8101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     8161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     8221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     8281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     8341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     8401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     8461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     8521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     8581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     8641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     8701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     8761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     8821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     8881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     8941 ggttccgcgc acatttcccc gaaaagtgcc acctggtcga cattgattat tgactagtta
     9001 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     9061 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     9121 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     9181 ggactattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     9241 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     9301 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggg
     9361 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
     9421 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg g
//
