LOCUS       sequence-17033-e         596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Ishida Y, Leder P
  TITLE     RET: a poly A-trap retrovirus vector for reversible disruption and 
            expression monitoring of genes in living cells.
  JOURNAL   Nucleic Acids Res 1999 Dec 15;27(24):e35.
  PUBMED    10572187
REFERENCE   2  (bases 1 to 596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1999-12-15"; volume: "27(24)"; pages: "e35"
COMMENT     Sequence Label: pRET.IS.IRES-EGFP N1
FEATURES             Location/Qualifiers
     source          1..596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(124..142)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(125..142)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(162..179)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    168..392
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 agctgtacaa gtaaagcgcc agcacagtgg gtcgacgata aaataaaaga ttttatttag
       61 tctccagaaa aaggggggaa tgaaagaccc cacctgtagg tttggcaagc tagagggccc
      121 tattctatag tgtcacctaa atgctagagc tcgctgatca gcctcgactg tgccttctag
      181 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac
      241 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca
      301 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag
      361 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccaggggctg
      421 catgaccagg ctcagctaat aaaggatccc tgcagtcgag cagtgtggtt ttcaagagga
      481 agcaaaaagc ctctccaccc aggcctggaa tgtttccacc caatgtcgag cagtgtggtt
      541 ttgcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac ccaatg
//
