LOCUS       sequence-17034-e         515 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Ishida Y, Leder P
  TITLE     RET: a poly A-trap retrovirus vector for reversible disruption and 
            expression monitoring of genes in living cells.
  JOURNAL   Nucleic Acids Res 1999 Dec 15;27(24):e35.
  PUBMED    10572187
REFERENCE   2  (bases 1 to 515)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1999-12-15"; volume: "27(24)"; pages: "e35"
COMMENT     Sequence Label: pRET.IS.IRES-EGFP N2
FEATURES             Location/Qualifiers
     source          1..515
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(72..89)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    78..302
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gctgtacaag taaagcgcca gcacagtggt cgagcatgca tctagagggc ccgtttaaac
       61 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc
      121 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga
      181 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcaaga
      241 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat
      301 ggcttctgaa gcggaaagaa ccaggggctg catgaccagg ctcaactaat aaaggatccc
      361 tgcagtcgag cagtgtggtt ttcaagagga agcaaaaagc ctctccaccc aggcctggaa
      421 tgtttccacc caatgtcgag caatgtggtt ttgcaagagg aagcaaaaag cctctccacc
      481 caggcctgga atgcttccac ccaatgtcga gcaaa
//
