LOCUS       sequence-17035-e         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Ishida Y, Leder P
  TITLE     RET: a poly A-trap retrovirus vector for reversible disruption and 
            expression monitoring of genes in living cells.
  JOURNAL   Nucleic Acids Res 1999 Dec 15;27(24):e35.
  PUBMED    10572187
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res"; date: "1999-12-15"; volume: "27(24)"; pages: "e35"
COMMENT     Sequence Label: pRET.IIS.IRES-EGFP
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    546..594
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     619..638
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 actgcgtgcn tccatcttgt tcaatggccg atcccatatt ggctgcacgg atcctgaacg
       61 gcagaggtta cggcagtttg tctctccccc ttccgggagc caccttcttc tccaaccgtc
      121 ccggtcgcgc tctcggcgct tctgaggaga gaactggctg agtgacgccc tttatagatt
      181 cgcccttgtg tcccgcccct tcctttcccg ccctcccttg cgctacgggg ccgcccgcac
      241 cggcctacac ggagcgcgcg cggcggagtt gttagcttgg gtcgtggacc tgaccccacg
      301 caacgcccaa aataataacc cccacgaacc ataaaccatt ccccatgggg gaccccgtcc
      361 ctaacccacg gggccagtgg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc
      421 tgggccttca cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg
      481 gccccaatgg ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg
      541 tttatgaaca aacgacccaa cacccgtgcg ttttattctg tctttttatt gccgtcatag
      601 cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt tagcctcccc catctcccgg
      661 gcaaacgtgc gcgccaggtc gcatatatgt ctcgngtatg gacccggggg tggtgacgtg
      721 ggtctgggac catcccggga ggtanagtng cagcagggcg tnccggcagc ccggcgggng
      781 attgg
//
