LOCUS       sequence-170355-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of CeTRIC-B1 in P.Pichia strains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Yang H, Hu M, Guo J, Ou X, Cai T, Liu Z
  TITLE     Pore architecture of TRIC channels and insights into their gating 
            mechanism.
  JOURNAL   Nature. 2016 Oct 27;538(7626):537-541. doi: 10.1038/nature19767. 
            Epub 2016 Oct 3.
  PUBMED    27698420
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature19767"; journalName: "Nature"; date: "2016-10-27- 
            27"; volume: "538"; issue: "7626"; pages: "537-541"
COMMENT     Sequence Label: pPICZA_CeTRIC-B1_Del
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             757..774
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accatggttg tcccagagtc cttccagctt gaccaggaga ttcttcttga cgcaggtgcc
       61 cagttgcata gattgaaaat gtacccttat ttcgatgttg ctcattattt gcttatgatt
      121 atcgaagtca gagatgactt gggttcagct gccagtattt tctctagaaa gcacccattg
      181 tcttgttggc tttcttccat gttgatgtgc tttgcagatg ctttcttggc caactttttg
      241 cttggagagc cagttattgc acctttcaaa agacatgatg acattatcct tgctactatt
      301 atctggtact tggttttcta tgcaccattt gacggaatct acaagatcgc taaaattaca
      361 cctgtcaagt gtgttttggc cgtcatgaag gaagttaaaa gagcatacaa ggtttcccat
      421 ggagtctcac acgcagctaa actttaccca aacagttata tcgttcaagt cttggttggt
      481 actgccaaag gtgcaggatc tggtattgtt agaactttgg aacagcttgt tagaggagtc
      541 tggttgccaa cacacaatga gttgcttaga ccttcctttg ctaccaaggc ctgtgttgtc
      601 gccgcatccg ttttggctct tgaaaaatca ggaacttact tgacagcccc tcatgatttg
      661 gtttatcttg tcattgttgg tttctttgtc tacttcaagc tttctgctgt tatcttgcac
      721 gtcactgacc catttgctcc tattgagaat ttgttccatc atcatcatca tcattga
//
