LOCUS       sequence-170369-        1630 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses RRvT, a tandem heterodimer red fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Wiens M, Shen Y, Li X, Salem M, Smisdom N, Zhang W, Brown A, 
            Campbell RE
  TITLE     A tandem green-red heterodimeric fluorescent protein with high FRET 
            efficiency.
  JOURNAL   Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492.
  PUBMED    27781394
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492."
COMMENT     Sequence Label: pBad-HisB-RRvT
FEATURES             Location/Qualifiers
     source          1..1630
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(242..260)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(1584..1601)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccga gctcgagaat ggtgagcaag
      121 ggcgaggagg tgatcaagga attcatgagg ttcaaggtga ggatggaggg ctccatgaat
      181 ggacatgagt ttgaaattga aggagaggga gagggacgcc cttatgaagg cacccagacc
      241 gccaagctga aggtgaccaa gggcggcccc ctgcccttcg cctgggacat tctgtcccct
      301 cagtttatgt atggatctaa ggcttatgtc aaacatcctg ctgatattcc cgactacaag
      361 aagctgagct tccccgaggg cttcaagtgg gagagggtta tgaacttcga agatggagga
      421 ctggtcacag tcacacagga ttcctccctg caggatggaa cactgattta caatgtgaag
      481 atgaggggca ccaactttcc acccgacggc cccgtgatgc aaaagaagac aatgggatgg
      541 gaggcttcca cagaacgcct gtatcctcgt gatggagtcc tgaaaggaga gatccaccag
      601 gccctgaagc tgaaggacgg cggccactac ctggtggagt ttaagaccat ttatatggct
      661 aaaaaacctg tccaactgcc tggatattat tatgtcgata caaaactgga catcaccagc
      721 cacaacgagg actacaccat cgtggagcag tacgagagga gcgagggccg ccatcatctg
      781 ttcctctatg gaatggatga actctataaa ggcagcaccg gcagcggcag ctccggcccc
      841 atggtctcca aaggagaaga agccattaaa gagtttatgc gcttcaaagt cagcatggaa
      901 ggcagcatga acggccacga gttcgagatc gagggcgagg gcgagggcag gccctacgag
      961 ggaacacaga cagctaaact gaaagtcaca aaaggaggac ctctgccttt cgcttgggat
     1021 atcctgagcc cccagttcat gtacggcagc aaggcctacg tgaagcaccc cgccgacatc
     1081 cctgattata aaaaactgtc ctttcctgaa ggattcagat gggaacgcgt catgaatttc
     1141 gaggacggcg gcctggtgac cgtgacccag gacagcagca tccaggacgg caccctgatc
     1201 tataaagtca aagtgcgcgg aacaaatttc cctcctgatg gacctgtcat gcagaaaaaa
     1261 accatgggct gggaagccag caccgagagg ctgtacccca gggacggcgt gctgaagggc
     1321 gaaattcatc aggctctgaa actgaaagat ggaggacatt atctggtcga attcaaaaca
     1381 atctacatgg ccaagaagcc cgtgcagctc cccggctact actacgtgga caccaagctg
     1441 gatattacat cccataatga agattataca gttgtcgaac agtatgaacg ctccgaagga
     1501 aggcaccacc tctttctgta cggcatggac gagctgtaca agtaaaagct tggctgtttt
     1561 ggcggatgag agaagatttt cagcctgata cagattaaat cagaacgcag aagcggtctg
     1621 ataaaacaga
//
