LOCUS       sequence-170371-        1631 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses GGvT, a tandem homodimer green fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1631)
  AUTHORS   Wiens M, Shen Y, Li X, Salem M, Smisdom N, Zhang W, Brown A, 
            Campbell RE
  TITLE     A tandem green-red heterodimeric fluorescent protein with high FRET 
            efficiency.
  JOURNAL   Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492.
  PUBMED    27781394
REFERENCE   2  (bases 1 to 1631)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Chembiochem. 2016 Oct 26. doi: 10.1002/cbic.201600492."
COMMENT     Sequence Label: pBad-HisB-GGvT
FEATURES             Location/Qualifiers
     source          1..1631
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..66
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(70..78,79..93)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     primer_bind     complement(1585..1602)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 atggggggtt ctcatcatca tcatcatcat ggtatggcta gcatgactgg tggacagcaa
       61 atgggtcggg atctgtacga cgatgacgat aaggatccga gctcgagaat ggtgagcaag
      121 ggcgaggaag tgatcaaaga gttcatgcgg ttcaaagtgc ggatggaagg cagcatgaac
      181 ggccacgagt tcgagatcga gggcgagggc gaaggcagac cttacgaggg aacccagacc
      241 gccaagctga aagtgaccaa gggcggaccc ctgcccttcg cctgggatat cctgagcccc
      301 ctgctgatgt acggcagcaa gatgtacgtg aagcaccccg ccgacatccc cgactacaag
      361 aagctgagct tccccgaggg cttcaaatgg gtgcgcgtga tgaacttcga ggacggcgga
      421 ctggtgaccg ccacacagga tagttctctg caggacggca ccctgatcta cgaagtgaag
      481 atgcggggca ccaacttccc ccccgatggc cccgtgatgc agaaaaagac catgggctgg
      541 gaggccagca ccgagagact gtaccctaga gatggcgtgc tgaagggcga gatccaccag
      601 gccctgaagc tgaaggatgg cggccactac ctggtggagt tcaagaccat ctacatggcc
      661 aagaaacccg tgcagctgcc cggctactac tacgtggaca ccaagctgga catcaccagc
      721 cacaacgagg actacacccg tgtggaacag tacgagcgga gcgagggccg gcaccacctg
      781 tttctgtacg acatggacga gctgtacaag ggcagcaccg gcagcggcag ctccggcccc
      841 atggtgtcta aaggcgaaga agtgatcaaa gagtttatgc gctttaaagt gcgcatggaa
      901 ggctccatga atgggcatga gtttgagatt gaaggggagg gggagggcag gccctatgag
      961 ggcacacaga cagctaaact gaaagtgaca aaagggggcc ctctgccttt tgcttgggac
     1021 atcctgtctc cactgctgat gtatgggtct aagatgtatg tgaaacatcc tgccgatatt
     1081 ccagattata agaagctgtc ctttccagag gggtttaaat gggtgcgcgt gatgaatttt
     1141 gaagatgggg gcctggttac agctacccag gacagtagcc tgcaggatgg aacactgatc
     1201 tatgaagtga aaatgagagg gaccaatttt ccccctgacg gacctgtgat gcagaaaaaa
     1261 acaatgggat gggaagcctc taccgagcgg ctgtacccaa gggacggggt gctgaaaggg
     1321 gaaattcatc aggctctgaa actgaaagac ggggggcatt atctggtgga gtttaaaaca
     1381 atctatatgg ctaaaaagcc tgtgcagctg cctgggtact attatgtgga tacaaaactg
     1441 gatatcacct ctcacaatga ggattataca agagtggaac agtatgagag atccgagggg
     1501 cgccatcatc tgtttctgta tgatatggat gaactgtata agtgataagc ttggctgttt
     1561 tggcggatga gagaagattt tcagcctgat acagattaaa tcagaacgca gaagcggtct
     1621 gataaaacag a
//
