LOCUS       sequence-170389-        1037 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfection of cells to express DsRed1 (Ex555, Em585) 
            localising to the mitochondrial matrix via the transit peptide from 
            human TXN2 (Thioredoxin)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Bohler P, Stuhldreier F, Anand R, Kondadi AK, Schlutermann D, 
            Berleth N, Deitersen J, Wallot-Hieke N, Wu W, Frank M, Niemann H, 
            Wesbuer E, Barbian A, Luyten T, Parys JB, Weidtkamp-Peters S, 
            Borchardt A, Reichert AS, Pena-Blanco A, Garcia-Saez AJ, Itskanov S,
            van der Bliek AM, Proksch P, Wesselborg S, Stork B
  TITLE     The mycotoxin phomoxanthone A disturbs the form and function of the 
            inner mitochondrial membrane.
  JOURNAL   Cell Death Dis. 2018 Feb 19;9(3):286. doi: 
            10.1038/s41419-018-0312-8.
  PUBMED    29459714
REFERENCE   2  (bases 1 to 1037)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2018-02-19"; volume: "9(3):286. doi"; pages: " 
            10.1038/s41419-018-0312-8"
COMMENT     Sequence Label: pMSCVpuro-Mito-DsRed1
FEATURES             Location/Qualifiers
     source          1..1037
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      243..252
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(249..251,252..254,255..929)
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(432..452)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     650..669
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     844..867
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ccttctctag gcgccggaat tagatctcga gctcaagctt cgaattcatg gctcagcgac
       61 ttcttctgag gaggttcctg gcctctgtca tctccaggaa gccctctcag ggtcagtggc
      121 cacccctcac ttccagagcc ctgcagaccc cacaatgcag tcctggtggc ctgactgtaa
      181 cacccaaccc agcccggaca atatacacca cgaggatctc cttgacacgg gatccaccgg
      241 tcgccaccat ggtgcgctcc tccaagaacg tcatcaagga gttcatgcgc ttcaaggtgc
      301 gcatggaggg caccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
      361 cctacgaggg ccacaacacc gtgaagctga aggtgaccaa gggcggcccc ctgcccttcg
      421 cctgggacat cctgtccccc cagttccagt acggctccaa ggtgtacgtg aagcaccccg
      481 ccgacatccc cgactacaag aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
      541 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggct
      601 gcttcatcta caaggtgaag ttcatcggcg tgaacttccc ctccgacggc cccgtaatgc
      661 agaagaagac catgggctgg gaggcctcca ccgagcgcct gtacccccgc gacggcgtgc
      721 tgaagggcga gatccacaag gccctgaagc tgaaggacgg cggccactac ctggtggagt
      781 tcaagtccat ctacatggcc aagaagcccg tgcagctgcc cggctactac tacgtggact
      841 ccaagctgga catcacctcc cacaacgagg actacaccat cgtggagcag tacgagcgca
      901 ccgagggccg ccaccacctg ttcctgtagc ggccgcgact ctagatcata atcagccata
      961 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     1021 aacataaaat gaatgca
//
