LOCUS       sequence-170392-        1713 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral transfection of cells to express ratiometric Pericam as 
            described by Nagai et al 2001 (PMID 11248055) localising to the 
            mitochondrial matrix via the transit peptide from human COX8A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Bohler P, Stuhldreier F, Anand R, Kondadi AK, Schlutermann D, 
            Berleth N, Deitersen J, Wallot-Hieke N, Wu W, Frank M, Niemann H, 
            Wesbuer E, Barbian A, Luyten T, Parys JB, Weidtkamp-Peters S, 
            Borchardt A, Reichert AS, Pena-Blanco A, Garcia-Saez AJ, Itskanov S,
            van der Bliek AM, Proksch P, Wesselborg S, Stork B
  TITLE     The mycotoxin phomoxanthone A disturbs the form and function of the 
            inner mitochondrial membrane.
  JOURNAL   Cell Death Dis. 2018 Feb 19;9(3):286. doi: 
            10.1038/s41419-018-0312-8.
  PUBMED    29459714
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Death 
            Dis."; date: "2018-02-19"; volume: "9(3):286. doi"; pages: " 
            10.1038/s41419-018-0312-8"
COMMENT     Sequence Label: pMSCVpuro-Mito-Pericam
FEATURES             Location/Qualifiers
     source          1..1713
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             36..110
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             132..206
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             237..314
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     CDS             357..608
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSFQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     545..566
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(678..699)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(939..958)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(1563..1586)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 ctccttctct aggcgccgga attagatccg ctagcatgtc cgtcctgacg ccgctgctgc
       61 tgcggggctt gacaggctcg gcccggcggc tcccagtgcc gcgcgccaag atccattcgt
      121 tgggggaccc catgtccgtc ctgacgccgc tgctgctgcg gggcttgaca ggctcggccc
      181 ggcggctccc agtgccgcgc gccaagatcc attcgttgcc gccggaggga gatctcaaga
      241 ggcgctggaa gaaaaacttc attgccgtca gcgctgccaa ccggttcaag aagatctcca
      301 gctccggggc actggggtct gcaggctaca acagcgacaa cgtctatatc atggccgaca
      361 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
      421 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      481 ccgacaacca ctacctgagc ttccagtccg ccctgagcaa agaccccaac gagaagcgcg
      541 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
      601 tgtacaaggt cgacggtggc agcggtggca ccggtgtgag caagggcgag gagctgttca
      661 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      721 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ctcatctgca
      781 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccttcggc tacggcctga
      841 agtgcttcgc ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      901 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      961 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     1021 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacggt accgaccaac
     1081 tgacagaaga gcagattgca gagttcaaag aagccttctc attattcgac aaggatgggg
     1141 acggcaccat caccacaaag gaacttggca ccgttatgag gtcgcttgga caaaacccaa
     1201 cggaagcaga attgcaggat atgatcaatg aagtcgatgc tgatggcaat ggaacgattt
     1261 actttcctga atttcttact atgatggcta gaaaaatgaa ggacacagac agcgaagagg
     1321 aaatccgaga agcattccgt gtttttgaca aggatgggaa cggctacatc agcgctgctc
     1381 agttacgtca cgtcatgaca aacctcgggg agaagttaac agatgaagaa gttgatgaaa
     1441 tgataaggga agcagatatc gatggtgatg gccaagtaaa ctatgaagag tttgtacaaa
     1501 tgatgacagc aaagtaagaa ttctaccggg taggggaggc gcttttccca aggcagtctg
     1561 gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc
     1621 gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg
     1681 cgccaccttc tactcctccc ctagtcagga agt
//
