LOCUS       sequence-170440-        2740 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-EGFP sequence for C-terminus tagging of 
            human PXN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2740)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-1:PXN-EGFP
FEATURES             Location/Qualifiers
     source          1..2740
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1019..1021,1022..1735)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(1019..1021,1022..1732)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1061..1082)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1322..1341)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1669..1690
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cctctgcctg ctgagttcca gtgattctcc cgcctcagcc tcccaagtag ctgagattac
       61 aggcacacgc ccccatgcct ggctaatttt ttgtattttc agtagagacg gggtttcacc
      121 atgttggcca agctggtctt gaactcctga cctcaggtga tccgcctccc ttggcctccc
      181 aaagtgctgg gattacaagt gtgagccacc tcacccggcc cctctcagag ccttttctac
      241 ctatatgtga tgtgaatctc caatgagaat ctaggaggca gagtttgact acagaccagt
      301 gtcacacctg tgtttctggg aacactgtta cagccacctg gctaagtgct caggagtcag
      361 acctgtgtat gaatccaggc tgtgacctca gtagctgcat gaccctgggc aagttacttc
      421 acctgtgtgc ttcagttgcc tcccctgttg ggagaactaa ataatcccag ccctgtggga
      481 ggccgaggtg ggaggattgc aggaggccac atttgaccag catgggcaat atagtgagac
      541 ccccatctct acaaaaaaaa tttatttaat aaaataaaaa tgaaaaatga gcgtttagga
      601 caacacggca catgggaaac gcctagcaag taggagtcac tccgagcgtg ccgactatgc
      661 ccacctcggc cccatcacac agggtgcagc tctagcccga ggggcagctc cctgagcccc
      721 tctctccgcc tggcaggaat gcttcacgcc attcgtgaac ggcagcttct tcgagcacga
      781 cgggcagccc tactgtgagg tgcactacca cgagcggcgc ggctcgctgt gttctggctg
      841 ccagaagccc atcaccggcc gctgcatcac cgccatggcc aagaagttcc accccgagca
      901 cttcgtctgt gccttctgcc tcaagcagct caacaagggc aacttcaagg agcagaacga
      961 caagccttac tgtcagaact gcttactcaa gctcttctgc ggtaccagcg gcggaagcgt
     1021 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     1081 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     1141 gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     1201 gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca tgaagcagca
     1261 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     1321 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     1381 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     1441 ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga agaacggcat
     1501 caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc tcgccgacca
     1561 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     1621 gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     1681 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agtaatgata
     1741 ggtgccctgc ccctgtctct gccccccttc cccagccagc atcaccaact gcgactgtga
     1801 cctagagact tcacccgggg gtgaaggggt aaacccgact gaaactggaa cccttgtcct
     1861 ccgctggtgc gggatggaca gagggccgtg aggggtcccc ctgcttgtct tcacccctgc
     1921 cagagcctct gggccccctc ctccctcctg tagctctccc taggctgccc actctccatc
     1981 ctccccaggg gtagaggctg ggggctccac cccagcccat gtacgtcccc acgaactggc
     2041 ctggccagca ccccacactg gagccatctc ttcctcatat ttcagcagtg cagccggggg
     2101 gcagggaagg gcaggcaggg tctgttgggg tctcttttta tccttattcc tcccccgacc
     2161 taattgtctt tgttctgtga ttattggggg acacccggct ccctccagac aatgccagca
     2221 taaatccatc catccaaagg cagagaacca aaggggccat ggaaggttct ctgtgctcct
     2281 cctacccttc cagtgcccta ggcctggcga ctgcccctgc cttttagacc cgccctcccc
     2341 ttttatacct gctcttgttc tactgagaaa agcctctcca gcaataatgt tttctagtca
     2401 cttcctccgt ctccgtgacg gcgtgcctgg acactgtacc gactttgata gatttctaca
     2461 ctgaggtttg aattcatatc gcctgagttg cttttacttc tctatacaaa atgattttga
     2521 agagatttta aagacgttcc cttttgtatt ctcttcctca tccaccgcca ctgggcctgt
     2581 cactgatggt ggctctggtg tgaagtttgc tttgtactga gggttggggt ggggaagcaa
     2641 tttgtatttt attgtttctt agcacaagca ggtgaactgg gagcagctct gtgactcccc
     2701 ctctttcact tcatagctca ccaggactgt tttataaact
//
