LOCUS       sequence-170441-        2750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for N-terminus (second exon)
            tagging of human TUBA1B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-4:TUBA1B-mEGFP
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1020..1022,1023..1733)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1062..1083)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1323..1342)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1670..1691
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gagtgttctt tttttgatga aagcaataag aggactgcgg aagagctccc tgtcaatgta
       61 ccgctctaca ccagtgtatt acgacagttc gtacacaaca gtctgtagag gccacctgtc
      121 tctccctgct gcgttaggaa ttcaggggag caggtggtgg cagtaaggga ttttgaggga
      181 acggaaatcg gatcttgacc cagatctggg ccgccgataa tctcctactg cgctcagact
      241 gctgtggagg tgttaggctg agcccgatgc cggcaggcaa gggaggatgg gcggcttggg
      301 cagcgccttt gcagacgtgg ccatttcgtg cctctgcagc accgccgggg ggcgcaagag
      361 cgcgcgcccg gaattgctca ttcatcctgt gccgcagagc cccgcccctt gtccctgcgg
      421 acagacattt cttctgcgct ggtctggcca cgtgcttcct gtgctaggag ctgcccggaa
      481 atgtgaccac ctagtctaaa gtgggcttct ggggcctgag cgctggatgg atgcccacct
      541 tcctgtcttg gtcctccaaa ggaggaagct gtgactgagc tgtcttggtc tggaaggagg
      601 ccttcccggt ttaggatggg aaggtaacat tcattaaaag caacgtagac tatagtgtag
      661 ctgttctcaa aagtagtaca tcttagaaaa ggatctttag aaaagatcgc tttagaaaag
      721 gaaattcgtt ttcagattac gtgagtagcc taggtaacac agccagacct catctccaca
      781 aaaaaaatga aaaaattagc cagcttggtg gtctgtgcct gtggtcccag ctgctccaga
      841 ggctgaggtg gggggatgac tggagcctag gctgcagtga gcctagatgg catcactgca
      901 ctcaagactg ggcgacagac cttatctcta aaaaaataaa gattgcatga gtattttgtt
      961 ccacttgaca gtcatcaata gattggttta aattgtgata tcttttttac ttaccgcagg
     1021 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     1081 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     1141 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     1201 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     1261 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca
     1321 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     1381 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     1441 tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca
     1501 tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc
     1561 actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc
     1621 tgagcaccca gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc
     1681 tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggaggtt
     1741 caggaggcag cgagtgcatc tccatccacg ttggccaggc tggtgtccag attggcaatg
     1801 cctgctggga gctctactgc ctggaacacg gcatccagcc cgatggccag atgccaagtg
     1861 acaagaccat tgggggagga gatgactcct tcaacacctt cttcagtgag acgggcgctg
     1921 gcaagcacgt gccccgggct gtgtttgtag acttggaacc cacagtcatt ggtgagttga
     1981 cctcagtaac ctgagatccc aggatgctgg gacaggaggt ctgtccaggg gcttctcttg
     2041 tcactcactc actccctccg tccttctctc cctcctccag atgaagttcg cactggcacc
     2101 taccgccagc tcttccaccc tgagcagctc atcacaggca aggaagatgc tgccaataac
     2161 tatgcccgag ggcactacac cattggcaag gagatcattg accttgtgtt ggaccgaatt
     2221 cgcaagctgg taagcaccac atataaatat gcatttaatg tggtgtgata gttccagtgc
     2281 aagttgggtg gagtgactga catcattcat tctttggcac ctaccaaaat gtggaatagg
     2341 ctgcttgcta tattaattgg acttctaaat cagatagtcc ctaggttatg gacagtttgt
     2401 ggatatgtct gttttgccaa ttccttgtgc ttacatcagt gagatatggt tcgtaatcta
     2461 aaaagttgaa atagaaattc taagataatg tgtcctggca ttaaaatatt acattttttt
     2521 attcccctac aggctgacca gtgcaccggt cttcagggct tcttggtttt ccacagcttt
     2581 ggtgggggaa ctggttctgg gttcacctcc ctgctcatgg aacgtctctc agttgattat
     2641 ggcaagaagt ccaagctgga gttctccatt tacccagcac cccaggtttc cacagctgta
     2701 gttgagccct acaactccat cctcaccacc cacaccaccc tggagcactc
//
