LOCUS       sequence-170442-        2743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for N-terminus tagging of 
            human LMNB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2743)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-10:LMNB1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1000..1002,1003..1005,1006..1716)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1045..1066)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1306..1325)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1653..1674
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 taaaggctgg tacttggaac ctgcaagccg tgcatttgga acctcggact caagtgccta
       61 ttacgtaatt ccacagcgtc ccggcctcca ggccgtttcc cgagccctcc agcggagcgg
      121 gggataaggt taccacgccc gcggtggccg gggacactct gagtttcgcg tgtggctttt
      181 agggacgttt atatttgaat ttccctgaac cgccgagtgt gggcggtggc gcagatccgt
      241 cccggaaacc tccgggctcc ttcccgcctt tctcaggccc ggcccctcca aggggtcccc
      301 gcggggcggc gggagggccc tgggcccaga gccgcgcggg tgggcagtcc caggcgtcct
      361 tccttacagc cctgagcctg gtccgggaac cgcccagccg ggagggccga gctgacggtt
      421 gcccaagggc cagattttaa atttacaggc ccggcccccg aaccgccgaa gcgcgctgcc
      481 tgctccccat tggcccatgg tagtcacgtg gaggcgccgg ggcgtgccgg ccatgttggg
      541 gagtgcggcg ccgcggcccg cgccacctcc gccccccgcg gcttgcctcc agcccgcccc
      601 tcccggccct cctccccccg cccgccgctc cgtgcagcct gagaggaaac aaagtgctgc
      661 gagcaggaga cggcggcggc gcgaaccctg ctgggcctcc agtcaccctc gtcttgcatt
      721 ttcccgcgtg cgtgtgtgag tgggtgtgtg tgttttctta caaagggtat ttcgcgatcg
      781 atcgattgat tcgtagttcc cccccgcgcg cctttgccct ttgtgctgta atcgagctcc
      841 cgccatccca ggtgcttctc cgttcctcta aacgccagcg tctggacgtg agcgcaggtc
      901 gccggtttgt gccttcggtc cccgcttcgc cccctgccgt cccctcctta tcacggtccc
      961 gctcgcggcc tcgccgcccc gctgtctccg ccgcccgcca tggtgagcaa gggcgaggag
     1021 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     1081 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     1141 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     1201 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     1261 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     1321 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     1381 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     1441 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     1501 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     1561 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccaag
     1621 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     1681 gccgggatca ctctcggcat ggacgagctg tacaagagcg gcctgagaag cagagcccag
     1741 gccagcgcga ctgcgacccc cgtgccgccg cggatgggca gccgcgctgg cggccccacc
     1801 acgccgctga gccccacgcg cctgtcgcgg ctccaggaga aggaggagct gcgcgagctc
     1861 aatgaccggc tggcggtgta catcgacaag gtgcgcagcc tggagacgga gaacagcgcg
     1921 ctgcagctgc aggtgacgga gcgcgaggag gtgcgcggcc gtgagctcac cggcctcaag
     1981 gcgctctacg agaccgagct ggccgacgcg cgacgcgcgc tcgacgacac ggcccgcgag
     2041 cgcgccaagc tgcagatcga gctgggcaag tgcaaggcgg aacacgacca gctgctcctc
     2101 aagtgagtgc tagctggcgg ccgcgttagc gccaaggagg ggcgggggcg caaccgcggc
     2161 gaccagctca ccgggttctg ccgtggggag ggagcagagg ccaggatgca cgcgtccttc
     2221 tgaaggaaca gggtctcggt ctccggaaag gagaaagaat ctagagttca tagcggagca
     2281 ggggtcgcgg agggggctcg agctgtagcg ctggggggcc gtgatgccca tttctagatt
     2341 ttggataccc gctgggacgt ggtaagtgcg cgcctgggac tgccgagaag gagctcccgc
     2401 tttcgcactc gaatccgggg agccggcgcg gagaggcggc ccctcaggcc ccaggtgcgg
     2461 ggagctggag cgcgagcgcg cgctcgcgtg cgcgccccag tttccggccg gcgcgagaca
     2521 aagcgtctag cggatttgca gtgccgggat gggcggccgg ggaggactgg cagcccgcct
     2581 ctagaatgaa tgagcttcgc gcgggcagag agaggaaggg gagggacctt cccgcagcat
     2641 ccgcgtctcc tgggggtggg tcccgctttg gcgcgctcag tcttggccct gtgacgtttt
     2701 gcgaagattc tacgcctgct ttaggcggga gagagaggcg gag
//
