LOCUS       sequence-170443-        2747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human TOMM20.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-8:TOMM20-mEGFP
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1031..1033,1034..1747)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1073..1094)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1334..1353)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1681..1702
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gatgatagga agtatttaca gaactttata gttagtaact gactggttaa tttttcaaac
       61 tgatttttac tcaactgaat tagaaaagga ctggaaagaa agtaaagatc ccaacgactt
      121 gagggaacaa gttggacaac caaggacttt gtctaaattg tttttattta gactaatgtg
      181 gttctagttc tagaggattc atactggaat catcggttta atattacgct atttgaaagg
      241 cagcatagta tagtactttt ggaaaattgg cctgagggtg atgtcttttg gaatatttgt
      301 gaattcacta tgaagcctaa ttccttaaaa atgacctcct tcactcaatt atcagtgttc
      361 ttggtttgcc tgggagtgaa aagagatctt aaaatctttt tggttttagt tacataattg
      421 actgatgtaa tattatgtaa tgatggctgt acacagtgtc tcatgcccta taatcctagc
      481 actcatttga gctcaggagt tcaaggccag cctgggcaac atggtaaaac cctgtctgca
      541 ccaaaaataa aaaaaaaatt agccgggcat ggtggcatga gtctgtggtt ccagctactc
      601 aggaggctga ggtgggggag gatcgcttga gcccaggagt cagaggttgc agtgagctga
      661 gattgtgcta ctgcactcca gcctgggtta cagagacccc atctgaatta aaaacatata
      721 taatgtaatg atctgcctcc tttgttaact tgacttttga aatgggattg tcagtagtat
      781 gatcattgtt ttcttggatg ccgactgtgt gtaaagtgtt acattttgaa ttaaatgtca
      841 gaatgggtga actttactaa gattcaattc tttgaataca aagagcattt tattttgaag
      901 ttagaatact aattaaatgc ttatgacact ttaaaaaatt attttttttt tctttcagag
      961 aattgtaagt gcacagtcct tggctgaaga tgatgtggaa ggcggtagcg gggatccacc
     1021 ggtcgccacc gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     1081 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     1141 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     1201 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     1261 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     1321 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     1381 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     1441 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     1501 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     1561 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     1621 caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga agcgcgatca
     1681 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     1741 caagtaatga gaaacaaatg tcaacataat aaaatctcag ttaaaaatat tttaaaaatt
     1801 cttggtagtt gagcagctct ggggtaataa gggcaaatat gcttgttatg aactacactg
     1861 aaatctacca aagttaatgt ttactttgtg tagatccatt tgtctatttt atttattttt
     1921 cccagtgaaa agtgtatttt gatagagaac ttttcattct ataaatacac tatgagttac
     1981 taaaatatca tggattttgt ttattcctga aacatagtta catagttaaa ctgtacatat
     2041 gacatggctt atgttaaaaa tacccagtgc tcagttttga aagataggca aaaaaaaaaa
     2101 agtataggag aaactgaaga atgtacactt ttttagaggg cacattttgc tgtaaatctg
     2161 gaaatttgat agacttgact gtgtttgtga aaactgagca ttaaaggttt tgattgatcc
     2221 tttctttcca tttaatctct gagacgtaaa tatgtgaggt gtgctgctgt gctgggttaa
     2281 cagcttcctt ccctttctgt gtagcagtct tgaaatgttc tgtttaaatc agtaggctta
     2341 atgtgttctg ggtatttatc tccttgtatt ttaaatatat gtagttgcaa atagcaccag
     2401 gaattagatt tctgtacacc cctaatctag ccttgtgagc ttcgctagtt aatgtgtgct
     2461 cactttccct ccatttgtta cgtgagagaa tgcgtctgct gatcactgaa gtgtcccttt
     2521 tagcttctga ttcattgggt tctgttgggc atctttaaat ccaccttaac ctgaggaatg
     2581 tatgtgggca accaggccct gcattttttt atattctgaa ttttgcatgc ttgcctgact
     2641 tagtatttct gaattgatgt tttttttaat ggtataacta tcttgatttt cactgaaatt
     2701 atatggttct gtcactactc tgtaaattaa tccgaaactt ttaaggt
//
