LOCUS       sequence-170444-        2738 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for C-terminus tagging of 
            human DSP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-9:DSP-mEGFP
FEATURES             Location/Qualifiers
     source          1..2738
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(1022..1024,1025..1738)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1064..1085)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1325..1344)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1672..1693
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tgttgacagg aagttctttg atcagtaccg atccggcagc ctcagcctca ctcaatttgc
       61 tgacatgatc tccttgaaaa atggtgtcgg caccagcagc agcatgggca gtggtgtcag
      121 cgatgatgtt tttagcagct cccgacatga atcagtaagt aagatttcca ccatatccag
      181 cgtcaggaat ttaaccataa ggagcagctc tttttcagac accctggaag aatcgagccc
      241 cattgcagcc atctttgaca cagaaaacct ggagaaaatc tccattacag aaggtataga
      301 gcggggcatc gttgacagca tcacgggtca gaggcttctg gaggctcagg cctgcacagg
      361 tggcatcatc cacccaacca cgggccagaa gctgtcactt caggacgcag tctcccaggg
      421 tgtgattgac caagacatgg ccaccaggct gaagcctgct cagaaagcct tcataggctt
      481 cgagggtgtg aagggaaaga agaagatgtc agcagcagag gcagtgaaag aaaaatggct
      541 cccgtatgag gctggccagc gcttcctgga gttccagtac ctcacgggag gtcttgttga
      601 cccggaagtg catgggagga taagcaccga agaagccatc cggaaggggt tcatagatgg
      661 ccgcgccgca cagaggctgc aagacaccag cagctatgcc aaaatcctga cctgccccaa
      721 aaccaaatta aaaatatcct ataaggatgc cataaatcgc tccatggtag aagatatcac
      781 tgggctgcgc cttctggaag ccgcctccgt gtcgtccaag ggcttaccca gcccttacaa
      841 catgtcttcg gctccgggct cccgctccgg ctcccgctcg ggatctcgct ccggatctcg
      901 ctccgggtcc cgcagtgggt cccggagagg aagctttgac gccacaggga attcttccta
      961 ctcttattca tactcattta gcagtagtag cattgggcac cacgaccccc ccgttgctac
     1021 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1081 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1141 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1201 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     1261 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     1321 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     1381 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     1441 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     1501 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     1561 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     1621 cctgagcacc cagtccaagc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     1681 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagtaata
     1741 gtagtcagtt gcgagtggtt gctatacctt gacttcattt atatgaattt ccactttatt
     1801 aaataataga aaagaaaatc ccggtgcttg cagtagagtg ataggacatt ctatgcttac
     1861 agaaaatata gccatgattg aaatcaaata gtaaaggctg ttctggcttt ttatcttctt
     1921 agctcatctt aaataagcag tacacttgga tgcagtgcgt ctgaagtgct aatcagttgt
     1981 aacaatagca caaatcgaac ttaggatttg tttcttctct tctgtgtttc gatttttgat
     2041 caattcttta attttggaag cctataatac agttttctat tcttggagat aaaaattaaa
     2101 tggatcactg atattttagt cattctgctt ctcatctaaa tatttccata ttctgtatta
     2161 ggagaaaatt accctcccag caccagcccc cctctcaaac ccccaaccca aaaccaagca
     2221 ttttggaatg agtctccttt agtttcagag tgtggattgt ataacccata tactcttcga
     2281 tgtacttgtt tggtttggta ttaatttgac tgtgcatgac agcggcaatc ttttctttgg
     2341 tcaaagtttt ctgtttattt tgcttgtcat attcgatgta ctttaaggtg tctttatgaa
     2401 gtttgctatt ctggcaataa acttttagac ttttgaagtg tttgtgtttt aatttaatat
     2461 gtttataagc atgtataaac atttagcata tttttatcat aggtctaaaa atatttgttt
     2521 actaaatacc tgtgaagaaa taccattaaa aaactatttg gttctgaatt cttactagaa
     2581 ggtggtcttt tgaagttagt cctttcggta cttctcagat gcctgtcatg tacccgatgg
     2641 agtccttgga aagaaggcct gtgtaaagag gccagcctgg aggtcaataa cctgttctag
     2701 tttattctgg acattgagta ccaagtagca ttggcaaa
//
