LOCUS       sequence-170445-        2729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for N-terminus tagging of 
            human ACTB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-15:ACTB-mEGFP
FEATURES             Location/Qualifiers
     source          1..2729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctcgagcgg ccgcggcggc gccctataaa acccagcggc gcgacgcgcc accaccgccg
       61 agaccgcgtc cgccccgcga gcacagagcc tcgcctttgc cgatccgccg cccgtccaca
      121 cccgccgcca ggtaagcccg gccagccgac cggggcaggc ggctcacggc ccggccgcag
      181 gcggccgcgg ccccttcgcc cgtgcagagc cgccgtctgg gccgcagcgg ggggcgcatg
      241 gggggggaac cggaccgccg tggggggcgc gggagaagcc cctgggcctc cggagatggg
      301 ggacacccca cgccagttcg gaggcgcgag gccgcgctcg ggaggcgcgc tccgggggtg
      361 ccgctctcgg ggcgggggca accggcgggg tctttgtctg agccgggctc ttgccaatgg
      421 ggatcgcagg gtgggcgcgg cggagccccc gccaggcccg gtgggggctg gggcgccatt
      481 gcgcgtgcgc gctggtcctt tgggcgctaa ctgcgtgcgc gctgggaatt ggcgctaatt
      541 gcgcgtgcgc gctgggactc aaggcgctaa ctgcgcgtgc gttctggggc ccggggtgcc
      601 gcggcctggg ctggggcgaa ggcgggctcg gccggaaggg gtggggtcgc cgcggctccc
      661 gggcgcttgc gcgcacttcc tgcccgagcc gctggccgcc cgagggtgtg gccgctgcgt
      721 gcgcgcgcgc cgacccggcg ctgtttgaac cgggcggagg cggggctggc gcccggttgg
      781 gagggggttg gggcctggct tcctgccgcg cgccgcgggg acgcctccga ccagtgtttg
      841 ccttttatgg taataacgcg gccggcccgg cttcctttgt ccccaatctg ggcgcgcgcc
      901 ggcgccccct ggcggcctaa ggactcggcg cgccggaagt ggccagggcg ggggcgacct
      961 cggctcacag cgcgcccggc tattctcgca actgacaatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caaggccggc tccggtaccg atgatgatat
     1741 cgcagcgctc gtcgtcgaca acggctccgg catgtgcaag gccggcttcg cgggcgacga
     1801 tgccccccgg gccgtcttcc cctccatcgt ggggcgcccc aggcaccagg taggggagct
     1861 ggctgggtgg ggcagccccg ggagcgggcg ggaggcaagg gcgctttctc tgcacaggag
     1921 cctcccggtt tccggggtgg gggctgcgcc cgtgctcagg gcttcttgtc ctttccttcc
     1981 cagggcgtga tggtgggcat gggtcagaag gattcctatg tgggcgacga ggcccagagc
     2041 aagagaggca tcctcaccct gaagtacccc atcgagcacg gcatcgtcac caactgggac
     2101 gacatggaga aaatctggca ccacaccttc tacaatgagc tgcgtgtggc tcccgaggag
     2161 caccccgtgc tgctgaccga ggcccccctg aaccccaagg ccaaccgcga gaagatgacc
     2221 caggtgagtg gcccgctacc tcttctggtg gccgcctccc tccttcctgg cctcccggag
     2281 ctgcgccctt tctcactggt tctctcttct gccgttttcc gtaggactct cttctctgac
     2341 ctgagtctcc tttggaactc tgcaggttct atttgctttt tcccagatga gctctttttc
     2401 tggtgtttgt ctctctgact aggtgtctaa gacagtgttg tgggtgtagg tactaacact
     2461 ggctcgtgtg acaaggccat gaggctggtg taaagcggcc ttggagtgtg tattaagtag
     2521 gcgcacagta ggtctgaaca gactccccat cccaagaccc cagcacactt agccgtgttc
     2581 tttgcacttt ctgcatgtcc cccgtctggc ctggctgtcc ccagtggctt ccccagtgtg
     2641 acatggtgta tctctgcctt acagatcatg tttgagacct tcaacacccc agccatgtac
     2701 gttgctatcc aggctgtgct atccctgta
//
