LOCUS       sequence-170446-        2680 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for N-terminus tagging of 
            human SEC61B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-7:SEC61B-mEGFP
FEATURES             Location/Qualifiers
     source          1..2680
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttaaatggg cccacactaa agttagagaa ccacaggctc gctcacaacc ctgacttctc
       61 catgtcagtt ccgatctttg cgaaccgcag acagggaagg tcttctctca ggggtcatgc
      121 ccgcggccgc cctccacggc gaggtccgca ctcgcgcagc cggccccgcg gccgcctcac
      181 ctggtcgcac actaccacgt cgaactcctc gtcggcgagg aacagcacgt agagcgccag
      241 gaaaaccatg cgcacgtagg cgcagacggc ggcgccgcgg ccgccccagc ccaggcctcg
      301 cggcagccag tccccggcac agcgcaccgg tagctcgcgg ctctcggcga aacagtggcc
      361 cgggtcgtag tgcgctgtcc agatcttcac gctacacccg cgcgcctgca gcgccagcgc
      421 cgcgtccaac accagccgct cagcgccgcc cacgcccagg tctgggtgga ggaacagcac
      481 cgacggcttg ggaaccgagt cccgttcccg gccctgctcc tccgccatgg ccctggagcc
      541 gcaactgcac cccgcaccct gatgggggtc ttctgcgcaa gctccgcgct cgtagctccc
      601 agctggccac tgcgggccga ccccgccctg ccgtacgtgc gtcagttagg ccacatcagc
      661 gcaaatctgt gagggtctag taactgcctg agaaaatatc ttgtctgacc ccggttatat
      721 ttttccttcg gtagggattg gactttctga aggacgttgt gatccaaagg aaggaggccg
      781 gaggtctcta cttcccatac agcaggtaac taagttgtct gtagcagact gtctacaggc
      841 atatcgtgag acgacccagg cgtccctggg gtcagagagg accttgcctg caagtccggg
      901 ggcggggcct gagtcagtct cgccagctgc cggtctttcg ggggctccgt aactttctat
      961 ccgtccgcgt cagcgccttg ccacactcat ctccaatatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagagcggc ctgagaagca tggtatggcg
     1741 gcccttccat gatccccgcc tctcccagaa gccctgactc ctcctgcttt gcgccgtgct
     1801 tttcctctgt agctcccttg cttcccccag cctcgggtgt gggtgtctag gccggggttc
     1861 tggggcaggc ctgccgcgct cacccgtctg tctgcttgtc tccctctaca gcctggtccg
     1921 acccccagtg gcactaacgt gggatcctca gggcgctctc ccagcaaagc agtggccgcc
     1981 cgggcggcgg gatccactgt ccggcagagg taaggaaccc tgcagttcgt tcgcttccag
     2041 actcggagat aggacccaga acctcgctga ttctggggtg gagaccctag catgtgaaga
     2101 ttgacaaagg caaaatgagc ttctagtgac gtggccgtgg gagtagttaa aggccttttg
     2161 ggaggaaggc gacatttttt ttctcgttgc tcagtttagg gcactactct taaaaaagga
     2221 aagttaacaa actggaatag agtcagagat aactttgaga aaaccgatgt cattaaactg
     2281 gtgtctctgg acctgaggtt tgcactcaca tttccatctg gcggccccat aagcaatctg
     2341 tcctacagat aactcgtcct acacaaaact tagtctcttt tcagctcagc tctctcactc
     2401 tcaattatat ctccttactt ccatatggca ctgttgtaca ctcatttact cagagccaga
     2461 aacgtcagcg tcatcttgga tttttcttat gctctttctc tctctagtca tatgccagac
     2521 tttaaactct gcttgaaagc tttctcataa gctctttcct tttccctttc tactgctttg
     2581 catttgctac ttaacccttt tcttcaggct gtttgctttc cagtccatcg ttcgctctgc
     2641 tgttactctt ctgcgtagtt tctgttactt gttgctgaac
//
