LOCUS       sequence-170448-        2747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for N-terminus tagging of 
            human MYH10.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-24:MYH10-mEGFP
FEATURES             Location/Qualifiers
     source          1..2747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(998..1000,1001..1003,1004..1714)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1043..1064)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1304..1323)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1651..1672
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aagtgatgga gcttcctctg ctagcccttt gtgagccaat ggtaaatggg tgctaaataa
       61 aacaactagg tcttgagata cattaattgt aaatgtcaca gaaccagtac tttcctcaat
      121 gtggctaaga tagttgatgg ttcctttttc ttctgcactg gtcagaccat atctgggcta
      181 tgatgtttgc ttctgggcca cacactttag agggaagaca agcagcatgt tggagtctgt
      241 ttaggcgaga gatccggcag gggaaggagt cttggtgaat gaggggtgga ggagctgcag
      301 gatgggaata gaggcctgaa ctgctaccat gacatattca aaaggctgcc gtgtgaagcc
      361 aagttatgct tgtcttttgt ggtcccagtt gatcacatta agacctcatg gggccataga
      421 aaagctagag ggagactgat ttgggttatt cataagaaga actttaagtc tgttatctga
      481 gggtagaatg agaggcatgt tttagattct ttagattctt tactcttctg acaatcatgt
      541 gtttttgtag ctgtttcctt gtggtcatat taattctggt accacttcat gaacctttta
      601 ttacccatct ttgttttctt tttttttttc ccttcttaac tccctgttta atttgtggtg
      661 agggtgaaag aggagataaa gaaaaaaaag ggtcaacttg taactttgcc ttttcttttc
      721 ttttcttttc ttttttttgc cctcagtaac tgagggcaaa cccatcagac aaccagagcc
      781 ataatttgtg gtcaccgctg aaatttacct tggaaactcg gttagtatgg ctgtgaagag
      841 gtatacccca gctccttaac acagagttaa tgcttaatct aaggttttaa gtttcttaga
      901 aaagaaaaac gtgtacattc ttttgtttct taaacatcta attttgccct cctcctcttc
      961 tcttagaggc aattgctttt ggatcgttcc atttacaatg gtgagcaagg gcgaggagct
     1021 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
     1081 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
     1141 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctacgg
     1201 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
     1261 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
     1321 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
     1381 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
     1441 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
     1501 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
     1561 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
     1621 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
     1681 cgggatcact ctcggcatgg acgagctgta caagtacagc gatctggagc tgaagctgcg
     1741 gatccctgca caaaggacag ggctggagga tccagagagg tacctgttcg tcgatcgtgc
     1801 tgtcatctac aaccctgcca ctcaagctga ttggacagct aaaaagctag tgtggattcc
     1861 atcagaacgc catggttttg aggcagctag tatcaaagaa gaacggggag atgaagttat
     1921 ggtggagttg gcagagaatg gaaagaaagc aatggtcaac aaagatgata ttcagaagat
     1981 gaacccacct aagttttcca aggtggagga tatggcagaa ttgacatgct tgaatgaagc
     2041 ttccgtttta cataatctga aggatcgcta ctattcagga ctaatctatg taagtatttc
     2101 ttccaaataa tcatgtgaag tggtagctag gaattaatgt aaattataca tcttgtcata
     2161 atcaaatgag aatgtggaat acccaaactc tctgtttaac atttctattt ctctttaaga
     2221 tagaaagatt tgttgcttgc ttacccatgt cttgcttttc tttgaatctt aacacattaa
     2281 gtttaaataa tacaggctgc aattacatat aataaaatgg catttgaaga cttttgtagt
     2341 ggtcttctgg agcataataa ggtgggagag agcatgtaac aggaagacca gaaggtttaa
     2401 taaggtaaag agagttgcat taattggacg cagacagcaa aacggtcaaa aatcaagtgc
     2461 atacccaaga gtaaagtgga ggggctgtaa gctgagaaat ttctgtggac agcatgaaca
     2521 gcttcactgg atgtagtagg gaagtaggaa agatgaatgc tgaggttttt aagaggaaac
     2581 aattagggta agattgaggc tggctggggt cgtcctgtgg ttagcagctg acatgaatgt
     2641 tggagtcacc gactttgtca ctgaccatgt agaagaagtt attgaaaatc ataagggata
     2701 atgtagagag ggataatgta gagaggaaaa atgtaagcca gatacta
//
