LOCUS       sequence-170449-        2750 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Homology arms and linker-mEGFP sequence for N-terminus tagging of 
            human TJP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Roberts B, Haupt A, Tucker A, Grancharova T, Arakaki J, Fuqua MA, 
            Nelson A, Hookway C, Ludmann SA, Mueller IA, Yang R, Horwitz R, 
            Rafelski SM, Gunawardane RN
  TITLE     Systematic gene tagging using CRISPR/Cas9 in human stem cells to 
            illuminate cell organization.
  JOURNAL   Mol Biol Cell. 2017 Oct 15;28(21):2854-2874. doi: 
            10.1091/mbc.E17-03-0209. Epub 2017 Aug 16.
  PUBMED    28814507
REFERENCE   2  (bases 1 to 2750)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E17-03-0209"; journalName: "Mol Biol Cell"; date: 
            "2017-10-15- 15"; volume: "28"; issue: "21"; pages: "2854-2874"
COMMENT     Sequence Label: AICSDP-23:TJP1-mEGFP
FEATURES             Location/Qualifiers
     source          1..2750
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(997..999,1000..1002,1003..1713)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1042..1063)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1303..1322)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1650..1671
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 agccttggca gtcggcgccg gtgaacgaga gcaacgcttc tgaccctgcc ggagctcctc
       61 ggagatgaaa gccatgacgc gccttgcaga aaatgcattc cgccttccgt gggaacaacg
      121 ccgaggcacg cggtgacagc cgtgaccatg ctgtttgccc agtgaaggaa acaactgtcg
      181 ggtatcggct ctgccggcct ttccagccgc actcatgcat ggggctcacc ccatgatgtg
      241 cgtggcttgt cgaggagcaa gtggacaagt ctcttaagga aagctttggt gcacaggcgc
      301 tttctccttg ggggcgaatt ctgccagacc ttggataaaa acaaacagga agactcgcac
      361 ggcagcggaa actgtcttcc aagttacttg ggttacccgg cttttccttc cgcgcttggg
      421 gtcgggaccc cggccgctcg tcccgccccc tcccccgccg cggccccgcc ccctccccgc
      481 ctcgcctcgc ctcgcctcgt ccagccccgc ccccgccggg ccgggcatgc tcagtgggcc
      541 gggccggcag gtttgcgtgg ccgctgagtt gccggcgccg gctgagccag cggacgccgc
      601 gttccttggc ggccgccggt tcccgggaag ttacgtggcg aagccggctt ccgaggagac
      661 gccgggaggc cacgggtgct gctgacgggc gggcgaccgg gcgaggccga cgtggccggg
      721 ctgcgaaagc tgcgggaggc cgagtgggtg gccgcgctcg gagggaggtg ccggtcgggc
      781 gcgccccgtg gagaagaccc gggcggggcg ggcgcttccc ggacttttgt ccgagttgaa
      841 ttccctcccc ctgggccggg cccttccggc cgcccccgcc cgtgccccgc tcgctctcgg
      901 gagatgttta tttgggctgt ggcgtgagga gcgggcgggc cagcgccgcg gagtttcggg
      961 tccgaggagt tgcgcgcggc gctggagaga gacaagatgg tgagcaaggg cgaggagctg
     1021 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1081 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1141 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     1201 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     1261 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     1321 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     1381 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     1441 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     1501 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     1561 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     1621 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     1681 gggatcactc tcggcatgga cgagctgtac aagagcggcc tgagaagcag agccctggag
     1741 agagacaaga gtgccagagc tgcggccgca aaggtgagaa cctccgcggc cgccagggcc
     1801 agaccgggcc gaccgtcgcc gcccgcccac cggcatctgg cccgcgtccc gccctccctc
     1861 gctggcggct gtctgggccc cggggcggcg gggtgggcag ggctggcgcg gggccgcggg
     1921 ccgcgggccg cgggccgcgg ggagcccctc gggcgggggc ggcgcgggcc gcactggggg
     1981 cggccgggga gggggctgcg ggcgcccggc cgccgtactg ggcaggtgca tagctgccgg
     2041 cgcctgtgcc tggctgcggc tcgctgaggg cggggacacg caacaggtcc ctcgcggaga
     2101 aactcggctc cagtgagggt tcgggggctg gaagccggct ctcagcgggt cggggcttgg
     2161 ggtgccacct cctgctggcc gggagctgct gtctttggag gagtggttgg tccccggcga
     2221 aaccctgtag tttcgatctg atgtcactcc ctgcggtatg cgcacgccag cgataaggct
     2281 ttgagactgc aaaacactcc actcagcctg tgaggcgtag taggtcgggt tttctttcat
     2341 gctgtattac ttatttaagg taactttgaa aataacctct ttaacattta ataatttaac
     2401 ttgaattaaa ctttcacaag taatacaaag tattcctacg aatggacaat aagatgagca
     2461 cttaaaaatt agtaaaggcc ggtgagttca gccgaaaaaa gtaacgtttt tcctgttact
     2521 tttcctatgt gctctgaaat attattgcat tttcccattg ctttgaaact aacttgtgta
     2581 ttacattaaa aagccaaagt tcctgaaaaa cagctaggat gctcctccca ttttgtatat
     2641 taattttttc atcataaaat agtacttgtt atttcaaaca aaggaataca gaaatgtgag
     2701 gagtaaaaaa tctccccttt aaagaatatc aattcattac ttcaaatagt
//
