LOCUS       sequence-170458-        6686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag tagged mouse Brd2 expression construct for cell transfection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6686)
  AUTHORS   Garcia-Gutierrez P, Mundi M, Garcia-Dominguez M
  TITLE     Association of bromodomain BET proteins with chromatin requires 
            dimerization through the conserved motif B.
  JOURNAL   J Cell Sci. 2012 Aug 1;125(Pt 15):3671-80. Epub 2012 May 17.
  PUBMED    22595521
REFERENCE   2  (bases 1 to 6686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-08-1"; volume: "125(Pt 15)"; pages: "3671-80. 
            Epub 2012 May 17"
COMMENT     Sequence Label: RSV-Flag-Brd2
FEATURES             Location/Qualifiers
     source          1..6686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   34..136
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     224..374
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     496..512
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     496..512
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        827..1088
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          1130..1226
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      1309..1318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1318..1341
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3826..3960
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3850..3869)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3904..3923
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4071..4087)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4071..4087)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4084..4106)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4095..4111
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4119..4125,4126..4143,4144..4149))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4164..4185
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4302..4319)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4473..5061)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4553..4572)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5232..6023,6024..6092))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5855..5874
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6093..6197)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6265..6283
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6321..6343)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6656..6678
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6670..6686,1)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(6671..6686,1)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaattcaag aattctcatg tttgacagct tatcatcatc aataatatac cttattttgg
       61 attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac
      121 ggggcgggtg acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg
      181 taagcgacgg atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga
      241 caattttcgc gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt
      301 tggccatttt cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac
      361 tcatagcgcg taatatttgt ctagggccgc ggggactttg accgtttacg tggagactcg
      421 cccaggtgtt tttctcaggt gttttccgcg ttccgggtca aagttggcgt tttattatta
      481 tagtcagggg gatcctctag aactagtgga tccctatggt gcactctcag tacaatctgc
      541 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      601 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      661 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      721 atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta cgcggttagg
      781 agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg tagtcttatg
      841 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      901 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      961 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattccgc attgcagaga
     1021 tattgtattt aagtgcctag ctcgatacaa taaacgccat ttgaccattc accacattgg
     1081 tgtgcacctc caagcttctc gaggaactga aaaaccagaa agttaactgg taagtttagt
     1141 ctttttgtct tttatttcag gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc
     1201 tcagtggatg ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc
     1261 tgcggaattg tacccgcggc cgcgcgccta ggagctcggt acccccgggc caccatggat
     1321 tacaaggatg acgacgataa gctagccggc acgggggtca agatgctgca aaacgtgact
     1381 ccccacaagc tccctgggga agggaatgca gggttactgg ggctgggccc agaggcagca
     1441 gcaccaggga aaaggattcg aaagccttct ctgctgtatg agggatttga gagccccaca
     1501 atggcttctg taccagcttt acaactggcc cctgccaatc caccaccccc tgaggtgtcc
     1561 aatcccaaaa agccaggacg ggtaacaaac caactgcagt acctgcacaa ggtagtgatg
     1621 aaggctctgt ggaagcatca gtttgcatgg ccattccggc agcctgtgga cgctgtgaag
     1681 ctgggtttgc cggattatca caaaattata aaacagccta tggacatggg tactatcaag
     1741 aggagacttg aaaacaatta ctactgggct gcctcagaat gtatgcagga ttttaatact
     1801 atgtttacca actgttacat ttataacaag cccaccgatg atattgtcct aatggcacag
     1861 acactggaaa agatcttcct acagaaagta gcatccatgc cacaagagga gcaagagctt
     1921 gtggtgacca tccctaaaaa cagccataag aagggggcca agttagcagc actccagggc
     1981 agtattacca gtgcccatca ggtgcctgct gtctcttctg tgtcgcatac agccctgtat
     2041 acaccaccac ctgaaatacc taccactgtc ctcaacattc cccacccatc agtcatctct
     2101 tctcctcttc ttaagtccct gcattctgct ggacccccac tccttgctgt atcagcagcg
     2161 cctccagctc agccccttgc caagaaaaaa ggcgttaaac ggaaagcgga tactaccacc
     2221 cctacaccca cagccatcct ggctcctggt tccccagcta gtcctcctgg gagtcttgag
     2281 ccaaaggcag caaggctccc tcctatgcgc agagagagtg gccgcccaat caaaccccca
     2341 cgaaaagact tgcctgactc gcaacagcaa caccagagct ctaagaaagg gaagctgtca
     2401 gagcagttaa agcactgcaa cggcatcctg aaggaactgc tctcaaagaa gcacgctgcc
     2461 tacgcctggc ccttctataa gccagtggac gcttctgctc ttggccttca tgattaccat
     2521 gacatcatta aacaccccat ggacctcagc actgtcaagc ggaagatgga gaaccgtgac
     2581 taccgggatg cacaggagtt tgctgctgat gtacggctta tgttctccaa ctgctataag
     2641 tacaatcctc cagaccacga tgttgtggct atggcacgaa agttgcagga tgtgtttgag
     2701 tttcgctatg ccaagatgcc agatgagcca ctggaaccag gacctctacc agtctctact
     2761 gccttgcctc ctgggttgac caaatcctct tcagagtcct ccagtgagga aagtagcagt
     2821 gagagttcct ctgaggaaga ggaggaggag gaagaagatg aggacgagga ggagagtgaa
     2881 agctcagact ctgaggagga aagggctcat cgcctagcag agctgcagga gcagcttcgg
     2941 gcagttcatg aacaactggc tgccctgtcc cagggcccaa tatctaagcc caagcggaag
     3001 agagagaaaa aggaaaaaaa gaagaaacgg aaggcagaga aacatcgtgg ccgaattggg
     3061 atcgatgaag atgataaggg gcctagggca cctcgcccac ctcagcccaa gaaatctaag
     3121 aaagcaggtg gtgggggtag caatgctact acactcagcc atcctggctt tgggacttcc
     3181 ggaggaagta gcaacaagct acctaaaaag tctcaaaaga cagctccacc tgtccttccc
     3241 actggctatg attctgagga ggaggaagaa agcaggccca tgagttatga tgagaagaga
     3301 cagttaagcc tggatatcaa taagttacct ggggaaaagc tgggtcgagt agtacatatc
     3361 atccaagcca gggaaccctc tctacgtgat tcaaatccag aagaaattga gattgatttt
     3421 gaaacactca agccgtccac acttagagag cttgagcgat atgttttatc ctgccttcga
     3481 aagaaacccc ggaagcccta cactattagg aaacctgtgg gaaaaacaaa ggaggaactg
     3541 gctttggaga agaagcggga gctagagaag cggttgcagg atgtcagtgg acagctcaac
     3601 tccaccaaaa agcctcccaa gaaagcgagt gagaagacag agtcatctgc acagcaagtg
     3661 gcagtgtccc gtctcagtgc ttctagttcc agctcagatt ccggctcctc gtcgtcatct
     3721 tcctcttctt cagacaccag cgattcagac tcgggctaag gggccagact agtgcggccg
     3781 ccacgtgatc gatgcataga tctagccggg ggatctggcc gcggggatcc agacatgata
     3841 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     3901 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     3961 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     4021 tcggatcctc tagagtcgac ctgcaggcat gcaagcttgg cgtaatcatg gtcatagctg
     4081 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
     4141 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     4201 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     4261 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     4321 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     4381 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     4441 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     4501 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     4561 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     4621 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     4681 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     4741 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     4801 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     4861 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     4921 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     4981 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     5041 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     5101 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     5161 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     5221 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     5281 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     5341 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta
     5401 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     5461 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     5521 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     5581 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     5641 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     5701 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     5761 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     5821 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     5881 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     5941 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     6001 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     6061 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     6121 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     6181 caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt
     6241 attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt
     6301 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
     6361 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
     6421 tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg
     6481 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ccattcgcca
     6541 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
     6601 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
     6661 tcacgacgtt gtaaaacgac ggccag
//
