LOCUS       sequence-170460-        6484 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Flag tagged mouse Brd3 expression construct for cell transfection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Garcia-Gutierrez P, Mundi M, Garcia-Dominguez M
  TITLE     Association of bromodomain BET proteins with chromatin requires 
            dimerization through the conserved motif B.
  JOURNAL   J Cell Sci. 2012 Aug 1;125(Pt 15):3671-80. Epub 2012 May 17.
  PUBMED    22595521
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2012-08-1"; volume: "125(Pt 15)"; pages: "3671-80. 
            Epub 2012 May 17"
COMMENT     Sequence Label: RSV-Flag-Brd3
FEATURES             Location/Qualifiers
     source          1..6484
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   34..136
                     /label=ITR
                     /note="inverted terminal repeat of human adenovirus 
                     serotype 5"
     misc_signal     224..374
                     /label=Ad5 Psi
                     /note="packaging signal for adenovirus serotype 5"
     primer_bind     496..512
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     496..512
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        827..1088
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     intron          1130..1226
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     regulatory      1309..1318
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1318..1341
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     polyA_signal    3624..3758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3648..3667)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3702..3721
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3869..3885)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3869..3885)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3882..3904)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3893..3909
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3917..3923,3924..3941,3942..3947))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3962..3983
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4100..4117)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4271..4859)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4351..4370)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5030..5821,5822..5890))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5653..5672
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5891..5995)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6063..6081
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6119..6141)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     6454..6476
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(6468..6484,1)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(6469..6484,1)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 tgaattcaag aattctcatg tttgacagct tatcatcatc aataatatac cttattttgg
       61 attgaagcca atatgataat gagggggtgg agtttgtgac gtggcgcggg gcgtgggaac
      121 ggggcgggtg acgtagtagt gtggcggaag tgtgatgttg caagtgtggc ggaacacatg
      181 taagcgacgg atgtggcaaa agtgacgttt ttggtgtgcg ccggtgtaca caggaagtga
      241 caattttcgc gcggttttag gcggatgttg tagtaaattt gggcgtaacc gagtaagatt
      301 tggccatttt cgcgggaaaa ctgaataaga ggaagtgaaa tctgaataat tttgtgttac
      361 tcatagcgcg taatatttgt ctagggccgc ggggactttg accgtttacg tggagactcg
      421 cccaggtgtt tttctcaggt gttttccgcg ttccgggtca aagttggcgt tttattatta
      481 tagtcagggg gatcctctag aactagtgga tccctatggt gcactctcag tacaatctgc
      541 tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag
      601 tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag
      661 aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt
      721 atctgagggg actagggtgt gtttaggcga aaagcggggc ttcggttgta cgcggttagg
      781 agtcccctca ggatatagta gtttcgcttt tgcataggga gggggaaatg tagtcttatg
      841 caatactctt gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag
      901 agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag
      961 gaaggcaaca gacgggtctg acatggattg gacgaaccac tgaattccgc attgcagaga
     1021 tattgtattt aagtgcctag ctcgatacaa taaacgccat ttgaccattc accacattgg
     1081 tgtgcacctc caagcttctc gaggaactga aaaaccagaa agttaactgg taagtttagt
     1141 ctttttgtct tttatttcag gtcccggatc cggtggtggt gcaaatcaaa gaactgctcc
     1201 tcagtggatg ttgcctttac ttctaggcct gtacggaagt gttacttctg ctctaaaagc
     1261 tgcggaattg tacccgcggc cgcgcgccta ggagctcggt acccccgggc caccatggat
     1321 tacaaggatg acgacgataa gctagccacg tgcgataatt cgattatgtc cactacagcg
     1381 gctgccccca cggggatccc ggcagtcccg ggccccgtga accctccccc acctgaggtc
     1441 tccaacccca gcaagcccgg gcgaaagact aaccaactgc agtacatgca gaatgtagtg
     1501 gtgaagacac tctggaaaca tcagtttgcc tggcctttct accagcctgt ggatgcaatc
     1561 aagctgaacc tgcctgatta tcataaaata ataaaaaacc caatggacat ggggactatc
     1621 aagaagagac tagaaaataa ttattattgg agtgccagtg agtgtatgca ggacttcaac
     1681 accatgttta caaactgtta tatttataat aagcccacag atgatatagt gctaatggcc
     1741 caggccttag agaaaatctt tctgcagaaa gtggcccaga tgcctcagga ggaagttgaa
     1801 ctattgcccc ctgctccaaa gggcaaaggc cggaagccag ctgcaggagc ccaaaatgca
     1861 ggttcccaac aagtggcagc tgtgtcttcg gtctccccag caaccccctt ccagaacata
     1921 ccccccaccg tgtcccagac acccgtcatt gccgctaccc ctgtaccaac catcactgca
     1981 aacgtcacgt cagttccagt ccccccaccc gctgcaccgc ctcctcctgc gacacccatc
     2041 gtccctgtgg tccctcccac accgcctgta gtcaagaaaa agggcgtgaa gcggaaagca
     2101 gacacaacca cacccacaac atctgccatc actgccagcc ggagtgagtc tcccccacca
     2161 ctttcagagc ccaagcaagc caaggtagtg gcccgaaggg agagcggggg ccgccccatc
     2221 aaacctccca agaaggacct ggaagatggt gaggtcccac agcacgcggg taaaaaggga
     2281 aaactgtccg agcacctgcg gcactgtgac agcatcctcc gggagatgct gtccaagaag
     2341 catgctgcct atgcgtggcc cttttacaag ccagtggacg cggaggcgct ggagctgcat
     2401 gactaccatg acatcatcaa gcaccccatg gacctcagca cggtcaaaag gaagatggat
     2461 agccgagagt acccagatgc acagggcttt gctgctgata tccggttaat gttctcgaat
     2521 tgttataagt acaaccctcc agaccacgaa gtggtggcca tggccaggaa gctccaggat
     2581 gtgtttgaga tgaggtttgc caagatgccc gatgagccca tggaggcgcc tgcgctgccc
     2641 gctcccacgg cccccatcgt gagcaagggg gctgagagca gccgcagtag tgaggagagc
     2701 tcttcagatt caggcagctc agactcagaa gaggagcgag ccactcggct ggctgagctg
     2761 caggagcagc tgaaggccgt gcacgaacag ctggctgcct tgtctcaggc cccagtgaac
     2821 aaaccaaaga agaagaaaga gaagaaggaa aaggaaaaga agaagaaaga caaggacaaa
     2881 gacaaagaga aggagaagca caaggccaag tctgaggagg agaagaaggc caaggcagcc
     2941 ccagcagcca agcaggccca gcagaaaaag gctcccacca agaaggccaa cagcacaacc
     3001 acagccagca gacagctcaa gaagggcggc aagcaggcat ctgcgtccta tgactcaggg
     3061 gaagaggagg agggcctgcc catgagctat gatgaaaagc gacaacttag ccttgacatc
     3121 aaccggctgc ccggcgagaa gctagggcgt gtggtgcaca tcattcagtc tcgggagccc
     3181 tcgcttcggg actcaaaccc agacgagatt gagattgact ttgagaccct gaagccaacc
     3241 acgctgcggg aactggagag atatgtcaag tcttgtttac aaaaaaagca gaggaaacca
     3301 ttgtcaacaa gcgggaagaa gcaggcagcc aaatcgaaag aggagctagc tcaggagaag
     3361 aaaaaggagc tggagaagcg gctgcaggat gtcagtggac aactgaacag caaaaaaccc
     3421 actaagaaag agaagtccgg ctcagctccc tcaggaggcc catcaaggct cagcagtagc
     3481 agctcctctg agtctgcgag cagtagctcc agtggatcga gctccgacag cagtgactca
     3541 gagtgaatca ctagtgaatt atcatacgta gatctatcga tgcatgaatg aatgacgatc
     3601 tagccggggg atctggccgc ggggatccag acatgataag atacattgat gagtttggac
     3661 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     3721 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     3781 ttatgtttca ggttcagggg gaggtgtggg aggttttttc ggatcctcta gagtcgacct
     3841 gcaggcatgc aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     3901 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     3961 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     4021 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     4081 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     4141 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     4201 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     4261 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     4321 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     4381 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     4441 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     4501 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     4561 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     4621 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     4681 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     4741 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     4801 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     4861 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     4921 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     4981 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     5041 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     5101 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     5161 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     5221 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     5281 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     5341 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     5401 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     5461 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     5521 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     5581 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     5641 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     5701 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     5761 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     5821 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     5881 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     5941 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     6001 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     6061 aaaataggcg tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc
     6121 tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     6181 gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg
     6241 cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat
     6301 gcgtaaggag aaaataccgc atcaggcgcc attcgccatt caggctgcgc aactgttggg
     6361 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg
     6421 caaggcgatt aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg
     6481 ccag
//
