LOCUS       sequence-170465-        9144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9144)
  AUTHORS   Gabut M, Samavarchi-Tehrani P, Wang X, Slobodeniuc V, O'Hanlon D, 
            Sung HK, Alvarez M, Talukder S, Pan Q, Mazzoni EO, Nedelec S, 
            Wichterle H, Woltjen K, Hughes TR, Zandstra PW, Nagy A, Wrana JL, 
            Blencowe BJ
  TITLE     An alternative splicing switch regulates embryonic stem cell 
            pluripotency and reprogramming.
  JOURNAL   Cell. 2011 Sep 30;147(1):132-46. Epub 2011 Sep 15.
  PUBMED    21924763
REFERENCE   2  (bases 1 to 9144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell."; 
            date: "2011-09-30"; volume: "147(1)"; pages: "132-46. Epub 2011 Sep 
            15"
COMMENT     Sequence Label: pLKO-tet-puro-sh-control
FEATURES             Location/Qualifiers
     source          1..9144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     76..93
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     CDS             complement(295..915)
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     823..842
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     promoter        complement(926..944)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(972..991)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          999..1571
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     promoter        complement(1580..2090)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1643..1661)
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     misc_feature    2139..2256
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2359..2453)
                     /label=H1-2O2 promoter
                     /note="doxycycline-inducible variant of the human H1 RNA 
                     promoter (Henriksen et al., 2007)"
     protein_bind    2359..2377
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2387..2405
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(2423..2442)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     CDS             complement(2742..2786)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2971..3204
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3697..3822
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             3869..4049
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4050..4276)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(4304..4324)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4304..4322)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4343..4359)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4343..4359)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4356..4378)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4367..4383
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4391..4397,4398..4415,4416..4421))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4436..4457
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4574..4591)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4745..5333)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4825..4844)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5504..6295,6296..6364))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6127..6146
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6365..6469)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6495..6950)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6632..6653)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6844..6863
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7077..7099
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7091..7108
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7092..7108
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7118..7136
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     rep_origin      7157..7292
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7332..7453
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7343..7362)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7397..7416
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             7525..7758
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     protein_bind    7902..8001
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(8030..8830)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="GSMIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQG
                     RPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEE
                     HQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQD
                     IALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(8141..8160)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     8751..8770
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     misc_feature    join(8831..9144,1..260)
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8991..9010)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
ORIGIN
        1 gggtcgctac agacgttgtt tgtcttcaag aagcttccag aggaactgct tccttcacga
       61 cattcaacag accttgcatt cctttggcga gaggggaaag acccctagga atgctcgtca
      121 agaagacagg gccaggtttc cgggccctca cattgccaaa agacggcaat atggtggaaa
      181 ataacatata gacaaacgca caccggcctt attccaagcg gcttcggcca gtaacgttag
      241 ggggggggga gggagagggg cagatcccta gaactcccgg gagccgctgt acgcggaccc
      301 actttcacat ttaagttgtt tttctaatcc gcatatgatc aattcaaggc cgaataagaa
      361 ggctggctct gcaccttggt gatcaaataa ttcgatagct tgtcgtaata atggcggcat
      421 actatcagta gtaggtgttt ccctttcttc tttagcgact tgatgctctt gatcttccaa
      481 tacgcaacct aaagtaaaat gccccacagc gctgagtgca tataatgcat tctctagtga
      541 aaaaccttgt tggcataaaa aggctaattg attttcgaga gtttcatact gtttttctgt
      601 aggccgtgta cctaaatgta cttttgctcc atcgcgatga cttagtaaag cacatctaaa
      661 acttttagcg ttattacgta aaaaatcttg ccagctttcc ccttctaaag ggcaaaagtg
      721 agtatggtgc ctatctaaca tctcaatggc taaggcgtcg agcaaagccc gcttattttt
      781 tacatgccaa tacaatgtag gctgctctac acctagcttc tgggcgagtt tacgggttgt
      841 taaaccttcg attccgacct cattaagcag ctctaatgcg ctgttaatca ctttactttt
      901 atctaatcta gacatatcaa ttcgccctat agtgagtcgt attacaattc tttgccaaaa
      961 tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca
     1021 tgaacatggt tagcagaggg gcccggtttg gactcagagt attttatcct catctcaaac
     1081 agtgtatatc attgtaacca taaagagaaa ggcaggatga tgaccaggat gtagttgttt
     1141 ctaccaataa gaatatttcc acgccagcca gaatttatat gcagaaatat tctaccttat
     1201 catttaatta taacaattgt tctctaaaac tgtgctgaag tacaatataa tataccctga
     1261 ttgccttgaa aaaaaagtga ttagagaaag tacttacaat ctgacaaata aacaaaagtg
     1321 aatttaaaaa ttcgttacaa atgcaagcta aagtttaacg aaaaagttac agaaaatgaa
     1381 aagaaaataa gaggagacaa tggttgtcaa cagagtagaa agtgaaagaa acaaaattat
     1441 catgagggtc catggtgata caagggacat cttcccattc taaacaacac cctgaaaact
     1501 ttgccccctc catataacat gaattttaca atagcgaaaa agaaagaaca atcaagggtc
     1561 cccaaactca cagatccccc tggggagaga ggtcggtgat tcggtcaacg agggagccga
     1621 ctgccgacgt gcgctccgga ggcttgcaga atgcggaaca ccgcgcgggc aggaacaggg
     1681 cccacactac cgccccacac cccgcctccc gcaccgcccc ttcccggccg ctgctctcgg
     1741 cgcgccctgc tgagcagccg ctattggcca cagcccatcg cggtcggcgc gctgccattg
     1801 ctccctggcg ctgtccgtct gcgagggtac tagtgagacg tgcggcttcc gtttgtcacg
     1861 tccggcacgc cgcgaaccgc aaggaacctt cccgacttag gggcggagca ggaagcgtcg
     1921 ccggggggcc cacaagggta gcggcgaaga tccgggtgac gctgcgaacg gacgtgaaga
     1981 atgtgcgaga cccagggtcg gcgccgctgc gtttcccgga accacgccca gagcagccgc
     2041 gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc ctcgagccgc
     2101 ggccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     2161 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     2221 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     2281 ctcgaggtcg agaattcaaa aagcaagctg accctgaagt tcatctcgag atgaacttca
     2341 gggtcagctt gcccggtgtc tctatcactg atagggaact tataagtctc tatcactgat
     2401 agggatttca cgtttatggt gatttcccag aacacatagc gacatgcaaa tattgcggcc
     2461 gctcgaggct agtctcgtga tcgataccgt cgagatccgt tcactaatcg aatggatctg
     2521 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
     2581 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
     2641 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
     2701 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
     2761 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
     2821 tgtattaagc ttgtgtaatt gttaatttct ctgtcccact ccatccaggt cgtgtgattc
     2881 caaatctgtt ccagagattt attactccaa ctagcattcc aaggcacagc agtggtgcaa
     2941 atgagttttc cagagcaacc ccaaatcccc aggagctgtt gatcctttag gtatctttcc
     3001 acagccagga ttcttgcctg gagctgcttg atgccccaga ctgtgagttg caacagatgc
     3061 tgttgcgcct caatagccct cagcaaattg ttctgctgct gcactatacc agacaataat
     3121 tgtctggcct gtaccgtcag cgtcattgac gctgcgccca tagtgcttcc tgctgctccc
     3181 aagaacccaa ggaacaaagc tcctattccc actgctcttt tttctctctg caccactctt
     3241 ctctttgcct tggtgggtgc tactcctaat ggttcaattt ttactacttt atatttatat
     3301 aattcacttc tccaattgtc cctcatatct cctcctccag gtctgaagat cagcggccgc
     3361 ttgctgtgcg gtggtcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
     3421 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
     3481 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
     3541 acagccttct gatgtttcta acaggccagg attaactgcg aatcgttcta gctccctgct
     3601 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
     3661 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
     3721 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
     3781 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
     3841 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
     3901 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
     3961 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
     4021 gctcagatct ggtctaacca gagagacccg tttatgtatc gagctaggca cttaaataca
     4081 atatctctgc aatgcggcaa ttcagtggtt cgtccaatcc atgtcagacc cgtctgttgc
     4141 cttcctaata aggcacgatc gtaccacctt acttccacca atcggcatgc acggtgcttt
     4201 ttctctcctt gtaaggcatg ttgctaactc atcgttacca tgttgcaaga ctacaagagt
     4261 attgcataag actacattaa gcttgcagct ccagcttttg ttccctttag tgagggttaa
     4321 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     4381 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     4441 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     4501 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     4561 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     4621 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     4681 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     4741 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     4801 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     4861 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     4921 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     4981 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     5041 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     5101 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     5161 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     5221 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     5281 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     5341 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     5401 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     5461 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     5521 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     5581 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     5641 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     5701 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     5761 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     5821 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     5881 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     5941 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     6001 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     6061 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     6121 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     6181 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     6241 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     6301 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     6361 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     6421 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     6481 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
     6541 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
     6601 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
     6661 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
     6721 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
     6781 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
     6841 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
     6901 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
     6961 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
     7021 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
     7081 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
     7141 attgggtacg tccctaggcc tccaaaaaag cctcctcact acttctggaa tagctcagag
     7201 gccgaggcgg cctcggcctc tgcataaata aaaaaaatta gtcagccatg gggcggagaa
     7261 tgggcggaac tgggcggagt taggggcggg atgggcggag ttaggggcgg gatagctaga
     7321 gccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     7381 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     7441 caataaacaa gttcctctca ctctctgata ttcatttctt tgcaagttat aaatactgaa
     7501 taataagatg acatgaacta ctactgctag agattttcca cactgactaa aagggtctga
     7561 gggatctcta gttaccagag tcacacaaca gacgggcaca cactacttga agcactcaag
     7621 gcaagcttta ttgaggctta agcagtgggt tccctagtta gccagagagc tcccaggctc
     7681 agatctggtc taaccagaga gacccagtac aagcaaaaag cagatcttgt cttcgttggg
     7741 agtgaattag cccttccagt cccccctttt cttttaaaaa gtggctaaga tctacagctg
     7801 ccttgtaagt cattggtctt aaaggtaccg atgcatgggg tcgtgcgctc ctttcggtcg
     7861 ggcgctgcgg gtcgtggggc gggcggatat cagctggatg gcaaataatg attttatttt
     7921 gactgatagt gacctgttcg ttgcaacaaa ttgataagca atgctttctt ataatgccaa
     7981 ctttgtacaa gaaagctggg tcggcgcgcc cacccttaaa aaaggtacct cagaagaact
     8041 cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca
     8101 cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg
     8161 ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc
     8221 ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct
     8281 cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat
     8341 gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct
     8401 cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc
     8461 gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga gatgacagga
     8521 gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt
     8581 cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt
     8641 cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct
     8701 gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat
     8761 agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa
     8821 tcatggatcc ttatcatcgt gtttttcaaa ggaaaaccac gtccccgtgg ttcggggggc
     8881 ctagacgttt ttttaacctc gactaaacac atgtaaagca tgtgcaccga ggccccagat
     8941 cagatcccat acaatggggt accttctggg catccttcag ccccttgttg aatacgcttg
     9001 aggagagcca tttgactctt tccacaacta tccaactcac aacgtggcac tggggttgtg
     9061 ccgcctttgc aggtgtatct tatacacgtg gcttttggcc gcagaggcac ctgtcgccag
     9121 gtggggggtt ccgctgcctg caaa
//
