LOCUS       sequence-170466-        6255 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Firefly luciferase reporter under control of the Rnd3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6255)
  TITLE     Moreau lab unpublished plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pGL4.10-Rnd3 promoter [-1759 +248]
FEATURES             Location/Qualifiers
     source          1..6255
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      2107..2116
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2113..3765
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    3809..3930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3820..3839)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3874..3893
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4178..4195)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4349..4937)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4429..4448)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5137..5928,5929..5997))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    6102..6150
                     /note="synthetic polyadenylation signal"
     misc_feature    6164..6255
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     6204..6223
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggcctaactg gccggtacct gagctctcct cataattacc attatgactc ctattttaca
       61 gatgatgaaa ttgaggctca gagaggctaa ataatccatc caaagtcata cagaaaatgt
      121 gaagttgggg acccaaatac acctctttgt gatctcctgc cccaactact atgcccattg
      181 ctctttaaac aagtaaacaa acgtgaataa tttgattaat gtttcctgtg ttaagtagtt
      241 ttgggaaatg ctaggttaaa ctatgttaaa tagtctgctt ttctctggga tttttaaatg
      301 tttgtaacat tagtgtgctt ggcgaatctt cagtgagtga tgtgggtaca gtgtcgccct
      361 cactaattgg gccatggcac cctttgtgtc aggagggaac taaagctcag aagaacaaac
      421 attggcaaag gctgctctag gaaccctagc agggccagca acccgcttgt ctgtgggtca
      481 accctcccct tgtgtaaaca cagacccacc gatggagtct gagtcaatga tctttgaggg
      541 ggctccaatt ttctcagccc caaataggac atcattcctg gatcccctag gcttcagggg
      601 ccctaccagt ccactactta catcagtgct tttgacaact cttcattgtg ccttttcact
      661 tcccatgtta aactttctga tttgtttgtt ttcaccttat cttctcattt acaagcctag
      721 gagtctccac aggggaccat ataagctggt tttggccagt gtgctactac attgtaacaa
      781 ttgaaacctc ctgagcattt agggtgcagg agctgaagat gagaaaaaga aagacttctc
      841 atagaaaaag aacaaatgtg cgggctattc tggtcctttc taagattttt gcttgcgggg
      901 aagagttgga gggctaggga gatggatgtg gaggaggaca gaagagaggg tggagggttg
      961 tatctgcatt tttcagctaa agatgtttgt gccttctgag tccagtctag ggtgctttga
     1021 ttccaggaag atggaggcta tagataaaga attaatagta tcaaaatctg cccccccacc
     1081 agtcccctca ctgcaaatgt ggggaggtgc aactaagctg acaagtgttt gggtccctag
     1141 agcagtaggg ttgagtttcc aatggccttt gtcactcagt catgggcatc ttccacttct
     1201 ctttcttctc ccctcccacc tcctgtaccc acccctcgaa acctttctgc ctccacttgt
     1261 acgactgaaa ctgacaggtt tcagaagcca gtctactacc gtgacccagg caagcctgtt
     1321 cttgcatctg gaataaagag gctgatctat tggtccatgg cctatgtacc tgatacaaac
     1381 cattaaaaaa aaaaatagaa ccactcaact attctccacc caagatttgc ttcctatgtt
     1441 ttatcactgt cctttaactt gggtgggaaa tgtttttgtt ttcataaatg ctcatgaggc
     1501 ttctacagat aagcccctat taaactatat ttaaagggat tgtagttcaa atcagtatgc
     1561 acagatacca gtttcccacc ttgccctgtg gacttgtcag caaagcatct ttgggatctt
     1621 catgttgtct aagagccttg gtctaaaata gatcccgaat gggcacccta gagggcgcac
     1681 tgcttacatt ttcggcatgc aacgccaaca gcgctcgctc ccaggctcca taaaagggga
     1741 ggaggcagat cagtttcctc ctctcattga gaaagaagag gattggaagg ccgggcgtat
     1801 aaagccgcgc agagagcgtg aaacaaagca gtcggctcgg aattggactt gggaggcgcg
     1861 gtgaggagtc aggcttaaaa cttgttggag gggagtaacc agcctgctcc tctcgctctc
     1921 ctcctcgtct gcgccgcgtt tcagaggttg cccatcagcc ttgtgattta tttttatatc
     1981 tgctttttat aaagagagaa atatatatat atatatatat tttttttttt tcttcttaag
     2041 agaaaattcc tgttccaaga gaaaataagg caacatcaag cttggcaatc cggtactgtt
     2101 ggtaaagcca ccatggaaga tgccaaaaac attaagaagg gcccagcgcc attctaccca
     2161 ctcgaagacg ggaccgccgg cgagcagctg cacaaagcca tgaagcgcta cgccctggtg
     2221 cccggcacca tcgcctttac cgacgcacat atcgaggtgg acattaccta cgccgagtac
     2281 ttcgagatga gcgttcggct ggcagaagct atgaagcgct atgggctgaa tacaaaccat
     2341 cggatcgtgg tgtgcagcga gaatagcttg cagttcttca tgcccgtgtt gggtgccctg
     2401 ttcatcggtg tggctgtggc cccagctaac gacatctaca acgagcgcga gctgctgaac
     2461 agcatgggca tcagccagcc caccgtcgta ttcgtgagca agaaagggct gcaaaagatc
     2521 ctcaacgtgc aaaagaagct accgatcata caaaagatca tcatcatgga tagcaagacc
     2581 gactaccagg gcttccaaag catgtacacc ttcgtgactt cccatttgcc acccggcttc
     2641 aacgagtacg acttcgtgcc cgagagcttc gaccgggaca aaaccatcgc cctgatcatg
     2701 aacagtagtg gcagtaccgg attgcccaag ggcgtagccc taccgcaccg caccgcttgt
     2761 gtccgattca gtcatgcccg cgaccccatc ttcggcaacc agatcatccc cgacaccgct
     2821 atcctcagcg tggtgccatt tcaccacggc ttcggcatgt tcaccacgct gggctacttg
     2881 atctgcggct ttcgggtcgt gctcatgtac cgcttcgagg aggagctatt cttgcgcagc
     2941 ttgcaagact ataagattca atctgccctg ctggtgccca cactatttag cttcttcgct
     3001 aagagcactc tcatcgacaa gtacgaccta agcaacttgc acgagatcgc cagcggcggg
     3061 gcgccgctca gcaaggaggt aggtgaggcc gtggccaaac gcttccacct accaggcatc
     3121 cgccagggct acggcctgac agaaacaacc agcgccattc tgatcacccc cgaaggggac
     3181 gacaagcctg gcgcagtagg caaggtggtg cccttcttcg aggctaaggt ggtggacttg
     3241 gacaccggta agacactggg tgtgaaccag cgcggcgagc tgtgcgtccg tggccccatg
     3301 atcatgagcg gctacgttaa caaccccgag gctacaaacg ctctcatcga caaggacggc
     3361 tggctgcaca gcggcgacat cgcctactgg gacgaggacg agcacttctt catcgtggac
     3421 cggctgaaga gcctgatcaa atacaagggc taccaggtag ccccagccga actggagagc
     3481 atcctgctgc aacaccccaa catcttcgac gccggggtcg ccggcctgcc cgacgacgat
     3541 gccggcgagc tgcccgccgc agtcgtcgtg ctggaacacg gtaaaaccat gaccgagaag
     3601 gagatcgtgg actatgtggc cagccaggtt acaaccgcca agaagctgcg cggtggtgtt
     3661 gtgttcgtgg acgaggtgcc taaaggactg accggcaagt tggacgcccg caagatccgc
     3721 gagattctca ttaaggccaa gaagggcggc aagatcgccg tgtaataatt ctagagtcgg
     3781 ggcggccggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3841 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3901 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3961 caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     4021 aaaatcgata aggatccgtc gaccgatgcc cttgagagcc ttcaacccag tcagctcctt
     4081 ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct ttatcatgca
     4141 actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac tcgctgcgct
     4201 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     4261 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     4321 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     4381 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     4441 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     4501 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     4561 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     4621 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     4681 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     4741 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg
     4801 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     4861 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     4921 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     4981 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     5041 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     5101 ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga tcagtgaggc
     5161 accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc ccgtcgtgta
     5221 gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga tgccgcgaga
     5281 gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga gggccgagcg
     5341 aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct gtcgtgatgc
     5401 tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg ctactggcat
     5461 cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc agcggtcaag
     5521 ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag ggcctccgat
     5581 cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag cactacacaa
     5641 ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt actcaaccaa
     5701 gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt ctatacggga
     5761 caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc gttcttcggg
     5821 gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc ccactcttgc
     5881 acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg caaaaacagg
     5941 caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga tgctcatact
     6001 cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct ctcaaggata
     6061 agtaagtaat attaaggtac gggaggtatt ggacaggccg caataaaata tctttatttt
     6121 cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac gctctccatc
     6181 aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca agtgcaggtg
     6241 ccagaacatt tctct
//
