LOCUS       sequence-170468-        9185 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based biosensor for monitoring cAMP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9185)
  AUTHORS   DiPilato LM, Zhang J
  TITLE     The role of membrane microdomains in shaping beta2-adrenergic 
            receptor-mediated cAMP dynamics.
  JOURNAL   Mol Biosyst. 2009 Aug;5(8):832-7. doi: 10.1039/b823243a. Epub 2009 
            May 26.
  PUBMED    19603118
REFERENCE   2  (bases 1 to 9185)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1039/b823243a"; 
            journalName: "Mol Biosyst"; date: "2009-08"; volume: "5"; issue: 
            "8"; pages: "832-7"
COMMENT     Sequence Label: pcDNA3-ICUE3
FEATURES             Location/Qualifiers
     source          1..9185
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..40)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        212..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     746..766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     840..859
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        840..858
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      878..887
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             899..916
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             920..952
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(956..964,965..979)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             join(986..988,989..991,992..1702)
                     /codon_start=1
                     /product="monomeric super enhanced variant of CFP (Matsuda 
                     et al., 2008)"
                     /label=mseCFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(986..988,989..991,992..1702)
                     /codon_start=1
                     /product="cyan variant of GFP"
                     /label=CFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(986..988,989..991,992..1702)
                     /codon_start=1
                     /product="enhanced CFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITHGMDELYK"
     CDS             join(4067..4069,4070..4072,4073..4585)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     promoter        complement(4715..4733)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4716..4733)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4753..4770)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4759..4983
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5029..5457
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5116..5135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5326..5347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5466..5486)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5471..5800
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5651..5786
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5713..5732
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5867..6661
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5921..5940)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6531..6550
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6835..6956
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6872..6891)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6926..6945
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7005..7021)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7005..7021)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7018..7040)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7029..7045
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7053..7059,7060..7077,7078..7083))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7098..7119
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7236..7253)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7407..7995)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7487..7506)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8166..8957,8958..9026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8789..8808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9027..9131)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cccctatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
       61 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
      121 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
      181 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
      241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      601 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      721 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      781 tatataagca gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt
      841 aatacgactc actataggga gacccaagct tgcggccgcc accatggtgc ggggttctca
      901 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      961 gtacgacgat gacgataagg atcccatggt gagcaagggc gaggagctgt tcaccggggt
     1021 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaggttca gcgtgtccgg
     1081 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1141 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctggggcg tgcagtgctt
     1201 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1261 ctacgtccag gagcgtacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1321 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1381 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacatcagcc acaacgtcta
     1441 tatcaccgcc gacaagcaga agaacggcat caaggcccac ttcaagatcc gccacaacat
     1501 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1561 ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc
     1621 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactca
     1681 cggcatggac gagctgtaca aggctggtac ccccgtggga actcatgaga tggaggagga
     1741 gttggccgaa gctgtggccc tgctctccca gcgggggcct gacgccctgc tcactgtggc
     1801 acttcgaaag cccccaggtc agcgcacgga tgaagagctg gacctcatct ttgaggagct
     1861 gctgcacatc aaggctgtgg cccacctctc caactcggtg aagcgagaat tagcggctgt
     1921 tctgctcttt gaaccacaca gcaaggcagg gaccgtgttg ttcagccagg gggacaaggg
     1981 cacttcgtgg tacattatct ggaagggatc tgtcaacgtg gtgacccatg gcaaggggct
     2041 ggtgaccacc ctgcatgagg gagatgattt tggacagctg gctctggtga atgatgcacc
     2101 ccgggcagcc accatcatcc tgcgagaaga caactgtcat ttcctgcgtg tggacaagca
     2161 ggacttcaac cgtatcatca aggatgtgga ggcaaagacc atgcggctgg aagaacatgg
     2221 caaagtggtg ctggtgctgg agagagcctc tcagggcgcc ggcccttccc gacccccaac
     2281 cccaggcagg aaccggtata cagtgatgtc tggcacccca gagaagatcc tagagcttct
     2341 gttggaggcc atgggaccag attccagtgc tcatgaccaa acagagacat tcctcagcga
     2401 cttcctcctg acccacaggg tcttcatgcc cagcgcccaa ctctgcgctg cccttctgca
     2461 ccacttccat gtggagcctg cgggtggcag cgagcaggag cgcagcacct acgtctgcaa
     2521 caagaggcag cagatcttgc ggctggtcag ccagtgggtg gccctgtatg gctccatgct
     2581 ccacactgac cctgtggcca ccagcttcct ccagaaactc tcagacctgg tgggcaggga
     2641 cacccgactc agcaacctgc tgagggagca gtggccagag aggcggcgat gccacaggtt
     2701 ggagaatggc tgtgggaatg catctcctca gatgaaggcc cggaacttgc ctgtttggct
     2761 ccccaaccag gacgagcccc ttcctggcag cagctgtgcc atccaagttg gggataaagt
     2821 cccctatgac atctgccggc cagaccactc agtgttgacc ctgcagctgc ctgtgacagc
     2881 ctccgtgaga gaggtgatgg cagcgttggc ccaggaggat ggctggacca aggggcaggt
     2941 gctggtgaag gtcaattctg caggtgatgc cattggcctg cagccagatg cccgtggtgt
     3001 ggccacatct ctggggctca atgagcgtct ctttgttgtc aacccacagg aagcgcatga
     3061 gctgatccca caccctgacc agctggggcc cactgtgggc tctgctgagg ggctggacct
     3121 ggtgagtgcc aaggacctgg caggccagct gacggaccac gactggagcc tcttcaacag
     3181 tatccaccag gtggagctga tccactatgt gctgggcccc cagcatctgc gggatgtcac
     3241 caccgccaac ctggagcgct tcatgcgccg cttcaatgag ctgcagtact gggtggccac
     3301 cgagctgtgt ctctgccccg tgcccggccc ccgggcccag ctgctcagga agttcattaa
     3361 gctggcggcc cacctcaagg agcagaagaa tctcaattcc ttctttgccg tcatgtttgg
     3421 cctcagcaac tcggccatca gccacctagc ccacacctgg gagcggctgc cccacaaagt
     3481 ccggaagctg tactccgccc tcgagaggct gctggacccc tcatggaacc accgggtata
     3541 ccgactggcc ctcgccaagc tctcccctcc tgtcatcccc ttcatgcccc ttcttctcaa
     3601 agacatgacc ttcattcatg agggaaacca cacactagtg gagaatctca tcaactttga
     3661 gaagatgaga atgatggcca gagtcgcgcg gatgctgcac cactgccgaa gccacaaccc
     3721 tgtgcctctc tcaccactca gaagccgagt ttcccacctc cacgaggaca gccaggtggc
     3781 gaggatttcc acatgctcgg agcagtccct gagcacccgg agtccagcca gcacctgggc
     3841 ttatgtccag cagctgaagg tcattgacaa ccagcgggaa ctctcccgcc tctcccgaga
     3901 gctggagcca gagctcatgc ccgacaacca ctacctgagc taccagtcca agctgagcaa
     3961 agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat
     4021 cactctcggc atggacgagc tgtacaaggg aggtaccggt ggatctatgg tgagcaaggg
     4081 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     4141 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     4201 gaagctgatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     4261 gggctacggc cttcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt
     4321 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     4381 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     4441 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     4501 ctacaacagc cacaacgtct atatcaccgc cgacaagcag aagaacggca tcaaggccaa
     4561 cttcaagatc cgccacaaca tcgaggacgg cggcgtgcag ctcgccgacc actaccagca
     4621 gaacaccccc atcggcgacg gccccgtgct gtaagaattc tgcagatatc catcacactg
     4681 gcggccgctc gagcatgcat ctagagggcc ctattctata gtgtcaccta aatgctagag
     4741 ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
     4801 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
     4861 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
     4921 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
     4981 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
     5041 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
     5101 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
     5161 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
     5221 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
     5281 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
     5341 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
     5401 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
     5461 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     5521 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     5581 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     5641 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
     5701 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
     5761 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
     5821 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
     5881 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
     5941 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
     6001 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
     6061 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
     6121 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
     6181 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
     6241 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
     6301 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
     6361 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
     6421 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
     6481 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
     6541 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
     6601 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
     6661 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6721 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     6781 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     6841 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6901 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6961 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7021 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7081 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7141 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7201 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7261 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7321 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7381 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7441 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7501 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7561 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7621 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7681 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7741 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7801 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7861 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7921 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7981 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8041 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8101 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8161 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8221 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8281 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8341 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8401 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8461 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8521 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8581 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8641 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8701 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8761 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8821 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8881 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8941 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9001 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9061 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9121 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     9181 ccgat
//
