LOCUS       sequence-170473-       12885 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For generating transgenic lines that ubiquitously expressing the 
            fluorescent MCP reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12885)
  AUTHORS   Campbell PD, Chao JA, Singer RH, Marlow FL
  TITLE     Dynamic visualization of transcription and RNA subcellular 
            localization in zebrafish.
  JOURNAL   Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. 
            Epub 2015 Mar 10.
  PUBMED    25758462
REFERENCE   2  (bases 1 to 12885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.118968"; journalName: "Development"; date: "2015-04-1- 
            1"; volume: "142"; issue: "7"; pages: "1368-74"
COMMENT     Sequence Label: pTol-Promoter:NLS-tdMCP-eGFP
FEATURES             Location/Qualifiers
     source          1..12885
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     30..52
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     44..61
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     45..61
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     71..90
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        71..89
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    join(219..230,231..393)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     protein_bind    765..785
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    6092..6116
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6149..6169
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6173..6199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             6173..6199
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6200..6211
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             join(6947..6949,6950..6952,6953..7666)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6947..6949,6950..6952,6953..7663)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6992..7013)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7253..7272)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7600..7621
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(7692..7716)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    7721..7855
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7745..7764)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7799..7818
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     misc_feature    join(9856..10043,10044..10055)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(10089..10105)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10089..10105)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(10142..10162)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(10142..10160)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(10181..10197)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10181..10197)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10194..10216)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10205..10221
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10229..10235,10236..10253,10254..10259))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10274..10295
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10412..10429)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10583..11171)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10663..10682)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11342..12133,12134..12202))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11965..11984
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(12203..12307)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(12333..12788)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12470..12491)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     12682..12701
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
       61 tgagcgcgcg taatacgact cactataggg cgaattgggt acaggtcatt gcacaacacc
      121 agaaatgccc tctgatctgc aaaagacgtg aatatctgtt cagacaccca tatccactct
      181 gttccacaca ggtcagaggt ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa
      241 aaattttact caagtgaaag tacaagtact tagggaaaat tttactcaat taaaagtaaa
      301 agtatctggc tagaatctta cttgagtaaa agtaaaaaag tactccatta aaattgtact
      361 tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac tagagattct tgtttaagct
      421 tttaatctca aaaaacatta aatgaaatgc atacaaggtt ttatcctgct ttagaactgt
      481 ttgtatttaa ttatcaaact ataagacaga caatctaatg ccagtacacg ctactcaaag
      541 ttgtaaaacc tcagatttaa cttcagtaga agctgattct caaaattgtt agtgtcaagc
      601 ctagctcttt tggggctgaa aagcaatcct gcagtgctga aaagcctctc acaggcagcc
      661 gatgcgggaa gaggtgtatt agtcttgata gagaggctgc aaatagcagg aaacgtgagc
      721 agagactccc tggtgtctga aacacaggcc agatgggccg atatcaactt tgtatagaaa
      781 agttgaaaat tccagtttga agaaactttt caagactcac cttttcactg ttggtcttaa
      841 tttaggttga ttatttttgt caatttaata attacattgt attgttcata ttttattgtc
      901 ttattgtggt gtttttacag cttttttatt gttctgcttt gttactgttt gccttgtatg
      961 cattgtagcg ctttgggtct gagaaaagcg cactataaat aaactgtatt attattatta
     1021 gggatggaca tcacggtggc gcgatgggtg gcacaattac ctcacagcaa gtaggttgct
     1081 gtttagagcc ccggctggat cagttggcag ttctgtgtgg agtttgcatg ttctccctgt
     1141 tcaagtaggt ttcctcccgg tgctctggtt tcccctcaca agtccaaaga catgatgtat
     1201 aggtgaatgg ggtaagctaa attgaccata gtgagtgtgt gtgtgtgtgt gaatgagagt
     1261 gtataggtgt ttcccagtgt taggttgcaa ctggaaggac atctgctgtg taaaacatat
     1321 gcaggataag ttggcggttc attcccctat ggtgacccct ggttaattaa aggactaagt
     1381 tgaaaatgaa tgaatgaatg aatgattaga gataaaccga taccactttt ttacaaaccg
     1441 atatgagtac aagtataata gtttctgtta ctcgacaata ctgagtaccg attctgatac
     1501 ttcttggatt tggtttcaat gtaattaatt tgaaagaagg ttttatttct tgcctgtcgc
     1561 aaggatgaac acaaaaatct ttaccagaag gatattttaa gctaagaata tacgcacgtt
     1621 gttgacaacc ctgtatcgat taccgattaa acgtggacat ttaaaggata agttatgctt
     1681 gcgccatcac aactttacac attacatgat caaagatcat attgaatatt cggtttattc
     1741 gtcagaagcc tatagatttg ttaaaagtgt attttataaa cggatggttt ataaagacta
     1801 agctattaat atatcagaca ttcattaata catcaagaca tcgatgtcag gtaggacaac
     1861 aaatcacatc catgtaatga aatcttgttg agtggtattg gtgcgtggtt tcagcatttc
     1921 atgtgcaata cgagtaagaa tattttaacc aagtatcgac ccaaaacctg atactggtat
     1981 cggtgcatcc cttattatta ttattatcaa aaacacactt gttttattta tgtgggtgcc
     2041 aaacaacaaa ctatgaaatg cagcatgaca cgcccaaaaa gtgttccttt gagaacaact
     2101 tcataaatat gtttcttatc attatgcatg aatggtgtac tgggagagct ttggtatcag
     2161 aattgagata agagactttg gcattctaaa ggacaatgac attatcacac tgacagtcaa
     2221 caacacttta ttgctctgga aatcttatgt catggggcaa agctgaggtt cattaggaca
     2281 gtaccttatt tagagatgaa tgggaaggct gtagtgggct gctatcaggg aagggtggtg
     2341 gtcctcgggg gaactatgga ctcacaaaag cacatctgct gtaacaagat cccctgtgca
     2401 atctacaaaa gttacaaaga gaagcatggt tggttaaaaa atagaattga ctggatatgc
     2461 tgaatgcatt gtttgttgtg tgttacatca cattaaccac agattggtgc taaatatttg
     2521 cttatcttat aaaaacctgt gtgtttataa aggggggtgt ggatggatgc ttggcattct
     2581 ttgaaaagat gttgggggat ggaaggagga gaccttattg atgagtatcc agtttgcaga
     2641 taacaagttt ttagacattc atactatgat gctgtttatg tctgtttttt ctcatttttt
     2701 ctatacacaa acagctataa aacattcact tttatatctg atgctgtata ttaaatttcg
     2761 ttgtatttat tctgtttgct ctgttgggtc atagctaggg ccagacagaa tctgctgaca
     2821 ttttttgctt tttctgcaga gaattttgta aacaatctgt gatttatgcg agattttttc
     2881 gggagtatcg taactaaaaa cttactatat gaaataaaaa aaaataatac ctttttaact
     2941 tttaataaat atatacaatg caagtccacc tagatccaat tatttggtcg acaaagcaag
     3001 tctctcacat aatatatctt ctaaaagaca gaaaacatta ctttcagacc atattgtaat
     3061 caaatgaaca ctttcatatt agtcaataat attacggaaa tgtatttaaa aactgaataa
     3121 acatatattt acacctttgt ctttatttct agagtgcttt tacaatgtag attgtgtcaa
     3181 agcagcttaa cttagaggat tagtatagta aattgaaact gtgtcagtcc attttttaag
     3241 agtttaagtt cagttcagtt tagttcagtg tgagttaatt tttactgctg agagtccaaa
     3301 cactaaagag caaatccaaa tacacaagtc aataaataaa ctcaatgatg ggctaaagat
     3361 ctggggaaat ctgcagattt ctgtgcgcac agattctgtg tgggcctagt cattacctat
     3421 actcaacgtt tactctgaga aacttccata ttttagaggt aaaatgctca gaattgtaaa
     3481 aagtcttgtc aaaatacttg gaatttgcca aaaacaaaat aaaagtcaca tcacctcatt
     3541 gtccttggta tcagcaagca cttttaacac aatcagctgt ttttgtttat aactcccact
     3601 gaaggccatg tgcattactc ccaagttaca cagtaaaaag tcacatgtag tttatcatga
     3661 tcactgctgc tcaagcacag ctttatctgt tattcagtgt ggcaatatct gaaatgtgtg
     3721 caaaatatta tttattgtag ctgatcacca acactttcag tgtttttttt ttctactttt
     3781 aatgagaatt atgttcttag gatttatcct tataaacctc tttaaaaaga aatgttatgt
     3841 ctgcatagac acgacatgaa attatgaaca gaaaatttaa ctatttaaga tcaagacttg
     3901 acatataaca tttctaatga acattaaacc tgaggtaggc ccacttgcaa aaatgtaact
     3961 gctatcactc actttataca tcattgtgta acttgtacac acaaaaatag tgaagcaatg
     4021 tggcatggaa tgcaggtccc attcattcaa agttattaaa gcactttctt aaagtgcatt
     4081 actactgagc taagaccagc tagttgctca tacaacatac tacaagtagt gtcatcctaa
     4141 tttggataaa aaaaatgtcc attttataca tggaaaagta ctcattagat cccccattgt
     4201 ttgtattacg gtatttcgtg aacacaagag gtaaatgacc tacagagctg ctgttgtgtt
     4261 agattgaaaa cacaacacag gatcatggag gcatccatca ttatgacgca ttctgccact
     4321 tgtaactctg cacagacttt ttgaaaagtt taattgagtc ttggcgttgt cgcagaaaaa
     4381 tacgaaaaat tctgtgttaa aatttgcaaa taaaaattcc agttttatag gaaatcgcca
     4441 tggccttgtt ctttaaccag ttaagcttcc ccttctttca ctctcaagtt gcaagaagca
     4501 agtgtagcaa tgtgcacgcg acagccgggt gtgtgacgct ggaccaatca gagcacagag
     4561 ctccgaaagt ttacctttta tggctagagc cgggtatgtg ccgtcatata aaagagcgcg
     4621 cccagctttt cagcctcact ttgagctcct ccacacgcag ctagtgcgga atatcatctg
     4681 cttgtaaccc attctcttaa gtcgacaacc ccccaaaccc aaggtgagtt ggttttacag
     4741 tttttaagcg tttaattata gtttctatct attaatgtta agtaaaatgt gtaaatggat
     4801 aaatatgatt tgatgaagtc tttagacagt tcataacggt ctctttgtag ttatctacta
     4861 ggtttgattt ataagtggtg gttcttttga taatctatgt tacatttaat aaatattggc
     4921 tgtatcttaa cttttttgta gcgttagtgg atgtggcagg tgagaatgtc ggtaatttaa
     4981 cgtgaccagt cgtaggcacg acattgaaca ttgaataggc cggtgtgaaa tgggtgttaa
     5041 gtcttaatat aaagttgtgt tctggctaat ctctctttaa cagccataaa tgtgattttt
     5101 aactacagtt ttgagggtgc tggtgagcat gttgcacact tgatggatag ccggcatggg
     5161 aagttctttg tgcaggcagt gctgcagcag ggtgtgacct actttagcta gccggctaac
     5221 cagctctcat ctgatgtaac gtaaacccca tcaatctaac atcggctcat tcgtgcttta
     5281 taagatcgaa gatcttgtta ttagtaacgg gtattggcat ttagttttaa cttggttaat
     5341 tttctttttg ttatgtgttc tatttaaatg tagcctgcag ccccctctgt ctttaatgaa
     5401 gctgaagatt aacgttaacg ttagtgaagt gttgtatccg ctatgaaaga tttgaatttt
     5461 agaggtgtgg tccactttaa taacggctgc ggagttctct ggctgcactt cagccgctgt
     5521 gactgtcgaa gcagagccca tcagtttttc aggatctcgg tgatttttga ggctagacta
     5581 tgaactgaac cgactgagag tgaccgtcca gaaatgcttt agtgtgaagt ctccgctcac
     5641 gaggagtccg tggactgact gcagatctgt gacgcagtaa cacccggcgg cagacacccg
     5701 ttgaaatgcg gttgtgtaat tgatcgcagg cgagggtgga agaggatgtg aaacttcatt
     5761 tgtgtaaaat ttagggagtg gtcctggtgt gatgaatgtc gaaatctgtt cctttttact
     5821 gagccctacg actctggctg agtgccacac cgccggcagc cgcaaagcgg ctcaaaccat
     5881 tgccttttat ggtaataatg agagaatgca gagggacttc ctttgtctgg catatctgag
     5941 gcgcgcgctg tcactagcgc ccaccagcgg tcagactgta gaatgcagag caaaacagga
     6001 agttgactcc agatggtcac atgcttgctg aaacgcctct ccctggcagc agtgcaattc
     6061 taacagtttc cctttctttt acagttcagc ctcaagtttg tacaaaaaag caggctccga
     6121 attcgccctt gagagactcg agatgggccc aaaaaagaaa agaaaagttg gctaccccta
     6181 cgacgtgccc gactacgcca tcgaaggccg ccatatgcta gccgttaaaa tggcttctaa
     6241 ctttactcag ttcgttctcg tcgacaatgg cggaactggc gacgtgactg tcgccccaag
     6301 caacttcgct aacgggatcg ctgaatggat cagctctaac tcgcgttcac aggcttacaa
     6361 agtaacctgt agcgttcgtc agagctctgc gcagaatcgc aaatacacca tcaaagtcga
     6421 ggtgcctaaa ggcgcctggc gttcgtactt aaatatggaa ctaaccattc caattttcgc
     6481 cacgaattcc gactgcgagc ttattgttaa ggcaatgcaa ggtctcctaa aagatggaaa
     6541 cccgattccc tcagcaatcg cagcaaactc cggcatctac gccatggctt ctaactttac
     6601 tcagttcgtt ctcgtcgaca atggcggaac tggcgacgtg actgtcgccc caagcaactt
     6661 cgctaacggg atcgctgaat ggatcagctc taactcgcgt tcacaggctt acaaagtaac
     6721 ctgtagcgtt cgtcagagct ctgcgcagaa tcgcaaatac accatcaaag tcgaggtgcc
     6781 taaaggcgcc tggcgttcgt acttaaatat ggaactaacc attccaattt tcgccacgaa
     6841 ttccgactgc gagcttattg ttaaggcaat gcaaggtctc ctaaaagatg gaaacccgat
     6901 tccctcagca atcgcagcaa actccggcat ctacgcggat tctagaatgg tgagcaaggg
     6961 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     7021 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     7081 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     7141 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     7201 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     7261 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     7321 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     7381 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     7441 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     7501 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     7561 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     7621 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaaccg gttctctcaa
     7681 gggcgaattc gacccagctt tcttgtacaa agttgatgta gatccagaca tgataagata
     7741 cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct ttatttgtga
     7801 aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac aagttaacaa
     7861 caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttaatt
     7921 cgcggccgcg gcgccaatgc attgggccct cgagcatatg agatctgtcc cgatctttct
     7981 cttctgtgct gtcaatttag agtagttttt gaggtaattt gggtgcattc tctgtaaaaa
     8041 caacaaataa atatttatat tgtatgcaat aaatgtaaca attacacata cactctctga
     8101 ttacagcatt aaagggttag ttcacccaaa aatgaaaaga tgtcattaat gactcaccct
     8161 catgtcgttc caagcccgta acacctccgt tcatcttcgg aacacagttt aagatatttt
     8221 agatttagtc cgagagcttt ctgtccctcc actgaaaatg tatgtacggt atactgtcca
     8281 tgtccagaaa ggtaataaaa acatcaaagt agtccatgtg acatcagtgg gttaggtaga
     8341 attatttgaa gcatcgaata cattttggtc caaaaataac aaaacctacg actttattcg
     8401 gcattgtatt ctcttccggg tctgttgtca atccgcgttc acgacttcgc agtgacgcta
     8461 caatgctgaa taaagtcgta ggttttgtta tttttggacc aaaatgtatt ttcgatgctt
     8521 caaataattc tacctaaccc actgatgtca catggactac tttgatgttt ttattacctt
     8581 tctggacatg gacagtatac cgtacataca ttctcaatgg aggcacagaa agctctcgga
     8641 ctaaatctaa aatatcttaa actgtgttct gaagatgaac ggaggtctta cgggcttgga
     8701 acgacatgag ggcgagtcat taatgacatt attttcattt ttgggtgaac taacccttta
     8761 ataaaactga catttgcagc caattgcaca ctttataatg ttaaatactt gttgatttta
     8821 gaaatacagt agcatttagt gacaatcaaa cattaatttt atttctgatt atattgacaa
     8881 aaacacagga ctgtaaggct gacatgacag ggtgaattga tttcattaca ttagttttga
     8941 tggtaacact tttacaataa ggttcgttag ttaactacat tagttaacat gaactaataa
     9001 tgaactgcac gtatacatgt taatttcaac atttactaat actttatatc ttgttaacat
     9061 tagttaatgc actgtgaact aacatgaaca aacaatgaac atctgtattt ctattaacta
     9121 acgttaacaa agattaacag atacagtaac aaatgtattg ctcatggtta tttaatgtta
     9181 gttaatacat gaactaatca acctcattgt aaagtgttac cgttttgaca atacaacatg
     9241 tttggaaaaa aatttctcgc ttggttttct tgtgttttgc atgaattcaa acacaatgag
     9301 agcacggaat gataatgatt attcaaagct gcaataggcg ccaaatttcc cgtggaccta
     9361 acgttaccaa ttacaaatgc attttagcat tttattacgt gagccttata ttgtcaatcg
     9421 catgcattag tgaaaatagg cctactacac atataaaagg cgcgccatca acgtcaaaac
     9481 atgcacaccc aaaaaaattg aatgaatctt tatttacacc acttaaagaa aagaattcgc
     9541 gaagatacgg ccacgggtgc tcttgatcct gtggctgatt ttggactgtg ctgctcgcag
     9601 ctgctgatga atcacatact tcctccattt tcttccactg attgactgtt ataatttccc
     9661 taatttccag gtcaaggtgc tgtgcattgt ggtaatagat gtgacatgac gtcacttcca
     9721 aaggaccaat gaacatgtct gaccaatttc atataatgtg aaaacgattt tcataggcag
     9781 aataaataac atttaaatta aactgggcat cagcgcaatt caattggttt ggtaatagca
     9841 agggaaaata gaatgaagtg atctccaaaa aataagtact ttttgactgt aaataaaatt
     9901 gtaaggagta aaaagtactt ttttttctaa aaaaatgtaa ttaagtaaaa gtaaaagtat
     9961 tgatttttaa ttgtactcaa gtaaagtaaa aatccccaaa aataatactt aagtacagta
    10021 atcaagtaaa attactcaag tactttacac ctctggttct tgacccccta ccttcagcaa
    10081 gcccagcaga tccactagtt ctagagcggc cgccaccgcg gtggagctcc agcttttgtt
    10141 ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt
    10201 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
    10261 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
    10321 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
    10381 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
    10441 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
    10501 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
    10561 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
    10621 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
    10681 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
    10741 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
    10801 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
    10861 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
    10921 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
    10981 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
    11041 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
    11101 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
    11161 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
    11221 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
    11281 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
    11341 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
    11401 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    11461 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    11521 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    11581 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    11641 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    11701 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    11761 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    11821 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    11881 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    11941 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    12001 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    12061 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    12121 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    12181 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    12241 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    12301 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
    12361 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
    12421 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
    12481 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
    12541 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
    12601 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
    12661 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
    12721 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
    12781 gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc
    12841 ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaag
//
