LOCUS       sequence-170474-        5782 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iDamID plasmid. To express transiently the c-Myc-tagged , 
            L122A-mutant E. coli Dam adenine methyltransferase fused to the 
            nuclear-localized mmGFP via flexylinker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J
  TITLE     iDamIDseq and iDEAR: An improved method and computational pipeline 
            to profile chromatin-binding proteins.
  JOURNAL   Development. 2016 Oct 5. pii: dev.139261.
  PUBMED    27707796
REFERENCE   2  (bases 1 to 5782)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Oct 5. pii: dev.139261."
COMMENT     Sequence Label: pCS2+ cMyc Dam_f_GFPoNLS
FEATURES             Location/Qualifiers
     source          1..5782
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..1046
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1081..1099
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1081..1098
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             1144..1173
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2107..2820
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2137..2165)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2760..2780
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    2880..3014
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2904..2923)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2958..2977
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3127..3147)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3127..3145)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3166..3182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3166..3182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3179..3201)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3190..3206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3214..3220,3221..3238,3239..3244))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3259..3280
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3397..3414)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3568..4156)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3648..3667)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4327..5118,5119..5187))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4950..4969
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5188..5292)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5318..5773)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5455..5476)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5667..5686
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
       61 agtcgaccgc caattcaata tggcgtatat ggactcatgc caattcaata tggtggatct
      121 ggacctgtgc caattcaata tggcgtatat ggactcgtgc caattcaata tggtggatct
      181 ggaccccagc caattcaata tggcggactt ggcaccatgc caattcaata tggcggacct
      241 ggcactgtgc caactgggga ggggtctact tggcacggtg ccaagtttga ggaggggtct
      301 tggccctgtg ccaagtccgc catattgaat tggcatggtg ccaataatgg cggccatatt
      361 ggctatatgc caggatcaat atataggcaa tatccaatat ggccctatgc caatatggct
      421 attggccagg ttcaatacta tgtattggcc ctatgccata tagtattcca tatatgggtt
      481 ttcctattga cgtagatagc ccctcccaat gggcggtccc atataccata tatggggctt
      541 cctaataccg cccatagcca ctcccccatt gacgtcaatg gtctctatat atggtctttc
      601 ctattgacgt catatgggcg gtcctattga cgtatatggc gcctccccca ttgacgtcaa
      661 ttacggtaaa tggcccgcct ggctcaatgc ccattgacgt caataggacc acccaccatt
      721 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc
      781 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag
      841 tacattacta ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc
      901 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa
      961 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct
     1021 atataagcaa tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg
     1081 atttaggtga cactatagaa tacaagctac ttgttctttt tgcaggatcc accggtcaac
     1141 atggagcaaa agctgatctc ggaggaggac ctgggcggct caggcaagaa aaatcgcgct
     1201 tttttgaagt gggcaggggg caagtatccc ctgcttgatg atattaaacg gcatttgccc
     1261 aagggcgaat gtctggttga gccttttgta ggtgccgggt cggtgtttct caacaccgac
     1321 ttttctcgtt atatccttgc cgatatcaat agcgacctga tcagtctcta taacattgtg
     1381 aagatgcgta ctgatgagta cgtacaggcc gcacgcgagc tgtttgttcc cgaaacaaat
     1441 tgcgccgagg tttactatca gttccgcgaa gagttcaaca aaagccagga tccgttccgt
     1501 cgggcggtac tgtttttata tttgaaccgc tacggttaca acggcgcttg tcgttacaat
     1561 ctgcgcggtg agtttaacgt gccgttcggc cgctacaaaa aaccctattt cccggaagca
     1621 gagttgtatc acttcgctga aaaagcgcag aatgcctttt tctattgtga gtcttacgcc
     1681 gatagcatgg cgcgcgcaga tgatgcatcc gtcgtctatt gcgatccgcc ttatgcaccg
     1741 ctgtctgcga ccgccaactt tacggcgtat cacacaaaca gttttacgct tgaacaacaa
     1801 gcgcatctgg cggagatcgc cgaaggtctg gttgagcgcc atattccagt gctgatctcc
     1861 aatcacgata cgatgttaac gcgtgagtgg tatcagcgcg caaaattgca tgtcgtcaaa
     1921 gttcgacgca gtataagcag caacggcggc acacgtaaaa aggtggacga actgctggct
     1981 ttgtacaaac caggagtcgt ttcacccgcg aaaaaagcta gcctgagcgg tggaggcggt
     2041 tcaggcggag gtggctctgg cggtggcgga tcgggaggcg gtggaagtgc agccgcgact
     2101 agtaccatga gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
     2161 gatggtgatg ttaatgggta caaattttct gtcagtggag agggtgaagg tgatgcaaca
     2221 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
     2281 acacttgtca ctactctcac ttatggtgtt caatgctttt caagatatcc agatcatatg
     2341 aagcggcacg acttcttcaa gagcgccatg cctgagggat acgtgcagga gaggaccatc
     2401 ttcttcaagg acgacgggaa ctacaagaca cgtgctgaag tcaagtttga gggagacacc
     2461 ctcgtcaaca ggatcgagct taagggaatc gatttcaagg aggacggaaa catcctcggc
     2521 cacaagttgg aatacaacta caactcccac aacgtataca tcatggccga caagcaaaag
     2581 aacggcatca aagccaactt caagacccgc cacaacatcg aagacggcgg cgtgcaactc
     2641 gctgatcatt atcaacaaaa tactccaatt ggcgatgacc ctgtcctttt accagacaac
     2701 cattacctgt ccacacaatc tgccctttcg aaagatccca acgaaaagag agaccacatg
     2761 gtccttcttg agtttgtaac ggctgctggg attacacatg gcatggatga actatacaaa
     2821 ccacctccca agaggcccag gctggactaa tctagaacta tagtgagtcg tattacgtag
     2881 atccagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     2941 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3001 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggagg
     3061 tgtgggaggt tttttaattc gcggccgcgg cgccaatgca ttgggcccgg tacccagctt
     3121 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
     3181 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggga gcataaagtg
     3241 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     3301 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     3361 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     3421 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     3481 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     3541 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     3601 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     3661 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     3721 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     3781 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     3841 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     3901 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     3961 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     4021 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     4081 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     4141 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     4201 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaagtatct tcacctagat
     4261 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     4321 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     4381 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     4441 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     4501 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     4561 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     4621 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     4681 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     4741 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     4801 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     4861 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     4921 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     4981 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     5041 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     5101 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     5161 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     5221 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     5281 aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt taatattttg
     5341 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
     5401 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
     5461 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
     5521 tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg
     5581 tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga
     5641 aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg
     5701 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
     5761 ctacagggcg cgtcccattc gc
//
