LOCUS       sequence-170478-        5741 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iDamID plasmid. To express transiently the optimized E. coli Dam 
            adenine methyltransferase fused to the nuclear-localized mmGFP via 
            flexylinker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5741)
  AUTHORS   Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J
  TITLE     iDamIDseq and iDEAR: An improved method and computational pipeline 
            to profile chromatin-binding proteins.
  JOURNAL   Development. 2016 Oct 5. pii: dev.139261.
  PUBMED    27707796
REFERENCE   2  (bases 1 to 5741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Oct 5. pii: dev.139261."
COMMENT     Sequence Label: pCS2+ oDam_f_GFPoNLS
FEATURES             Location/Qualifiers
     source          1..5741
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..1013
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1048..1066
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1048..1065
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             2033..2746
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(2063..2091)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     2686..2706
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    2806..2940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2830..2849)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2884..2903
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(3053..3073)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(3053..3071)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(3092..3108)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3092..3108)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3105..3127)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3116..3132
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3140..3146,3147..3164,3165..3170))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3185..3206
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3323..3340)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3494..4082)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3574..3593)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4253..5044,5045..5113))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4876..4895
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5114..5218)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5244..5699)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5381..5402)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5593..5612
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccagt cgaccgccaa ttcaatatgg cgtatatgga
       61 ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa ttcaatatgg cgtatatgga
      121 ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa ttcaatatgg cggacttggc
      181 accatgccaa ttcaatatgg cggacctggc actgtgccaa ctggggaggg gtctacttgg
      241 cacggtgcca agtttgagga ggggtcttgg ccctgtgcca agtccgccat attgaattgg
      301 catggtgcca ataatggcgg ccatattggc tatatgccag gatcaatata taggcaatat
      361 ccaatatggc cctatgccaa tatggctatt ggccaggttc aatactatgt attggcccta
      421 tgccatatag tattccatat atgggttttc ctattgacgt agatagcccc tcccaatggg
      481 cggtcccata taccatatat ggggcttcct aataccgccc atagccactc ccccattgac
      541 gtcaatggtc tctatatatg gtctttccta ttgacgtcat atgggcggtc ctattgacgt
      601 atatggcgcc tcccccattg acgtcaatta cggtaaatgg cccgcctggc tcaatgccca
      661 ttgacgtcaa taggaccacc caccattgac gtcaatggga tggctcattg cccattcata
      721 tccgttctca cgccccctat tgacgtcaat gacggtaaat ggcccacttg gcagtacatc
      781 aatatctatt aatagtaact tggcaagtac attactattg gaagtacgcc agggtacatt
      841 ggcagtactc ccattgacgt caatggcggt aaatggcccg cgatggctgc caagtacatc
      901 cccattgacg tcaatgggga ggggcaatga cgcaaatggg cgttccattg acgtaaatgg
      961 gcggtaggcg tgcctaatgg gaggtctata taagcaatgc tcgtttaggg aaccgccatt
     1021 ctgcctgggg acgtcggagc aagcttgatt taggtgacac tatagaatac aagctacttg
     1081 ttctttttgc aggatccacc ggtcaaacat gaagaagaac agggccttcc tgaagtgggc
     1141 tggcggaaag tacccactgc tggacgacat caagaggcac ctgcccaagg gcgagtgcct
     1201 ggtcgagcct ttcgtgggag ccggaagcgt gttcctgaac accgacttca gccgctacat
     1261 cctggccgac atcaacagcg acctgatcag cctgtacaac atcgtgaaga tgaggaccga
     1321 cgagtacgtg caggccgctc gcgagctgtt cgtgcccgag acaaactgcg ccgaggtgta
     1381 ctaccagttc agagaagagt tcaacaagag ccaggacccc ttccgcaggg ccgtgctgtt
     1441 cctgtacctg aacagatacg gatacaacgg cgcctgcaga tacaacctga ggggcgagtt
     1501 caacgtgccc ttcggaagat acaagaagcc ctacttcccc gaggccgagc tgtaccactt
     1561 cgccgagaag gcccagaacg cattcttcta ctgcgagagc tacgccgaca gcatggccag
     1621 ggccgacgac gcctctgtgg tgtactgcga ccctccctac gcccctctga gcgccaccgc
     1681 caacttcacc gcctaccaca ccaacagctt caccctggag cagcaggccc acctggccga
     1741 gatcgctgag ggactggtcg agaggcacat ccccgtgctg atctccaacc acgacaccat
     1801 gctgaccaga gagtggtatc agagggccaa gctgcacgtg gtcaaagtgc gcagatccat
     1861 cagcagcaac ggcggaacca gaaagaaggt ggacgagctg ctggccctgt acaagcccgg
     1921 cgtggtgtcc cctgccaaga aggctagcct gagcggtgga ggcggttcag gcggaggtgg
     1981 ctctggcggt ggcggatcgg gaggcggtgg aagtgcagcc gcgactagta ccatgagtaa
     2041 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
     2101 tgggtacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
     2161 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
     2221 tctcacttat ggtgttcaat gcttttcaag atatccagat catatgaagc ggcacgactt
     2281 cttcaagagc gccatgcctg agggatacgt gcaggagagg accatcttct tcaaggacga
     2341 cgggaactac aagacacgtg ctgaagtcaa gtttgaggga gacaccctcg tcaacaggat
     2401 cgagcttaag ggaatcgatt tcaaggagga cggaaacatc ctcggccaca agttggaata
     2461 caactacaac tcccacaacg tatacatcat ggccgacaag caaaagaacg gcatcaaagc
     2521 caacttcaag acccgccaca acatcgaaga cggcggcgtg caactcgctg atcattatca
     2581 acaaaatact ccaattggcg atgaccctgt ccttttacca gacaaccatt acctgtccac
     2641 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
     2701 tgtaacggct gctgggatta cacatggcat ggatgaacta tacaaaccac ctcccaagag
     2761 gcccaggctg gactaatcta gaactatagt gagtcgtatt acgtagatcc agacatgata
     2821 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
     2881 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
     2941 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
     3001 taattcgcgg ccgcggcgcc aatgcattgg gcccggtacc cagcttttgt tccctttagt
     3061 gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     3121 atccgctcac aattccacac aacatacgag ccgggagcat aaagtgtaaa gcctggggtg
     3181 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     3241 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     3301 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     3361 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     3421 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3481 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3541 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3601 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3661 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3721 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3781 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3841 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3901 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3961 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     4021 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     4081 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     4141 aagggatttt ggtcatgaga ttatcaaaaa gtatcttcac ctagatcctt ttaaattaaa
     4201 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     4261 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     4321 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     4381 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     4441 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4501 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4561 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4621 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4681 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4741 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4801 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4861 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4921 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4981 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     5041 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     5101 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     5161 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     5221 catttccccg aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt
     5281 aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     5341 taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     5401 actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     5461 cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     5521 aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     5581 ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     5641 ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     5701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga t
//
