LOCUS       sequence-170479-       11579 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  iDamID plasmid. To stably and ubiquitously express the c-Myc-tagged 
            and optimized E. coli Dam adenine methyltransferase fused to the 
            nuclear-localized mmGFP via flexylinker..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11579)
  AUTHORS   Gutierrez-Triana JA, Mateo JL, Ibberson D, Ryu S, Wittbrodt J
  TITLE     iDamIDseq and iDEAR: An improved method and computational pipeline 
            to profile chromatin-binding proteins.
  JOURNAL   Development. 2016 Oct 5. pii: dev.139261.
  PUBMED    27707796
REFERENCE   2  (bases 1 to 11579)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2016 Oct 5. pii: dev.139261."
COMMENT     Sequence Label: pDestTol2 ZfUbi::cMyc-oDam_f_GFPoNLS
FEATURES             Location/Qualifiers
     source          1..11579
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             13..42
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             976..1689
                     /codon_start=1
                     /gene="mgfp5"
                     /product="GFP with folding enhancement mutations"
                     /label=mgfp5
                     /note="suitable for expression in plants due to removal of 
                     a cryptic intron"
                     /translation="MSKGEELFTGVVPILVELDGDVNGYKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKA
                     NFKTRHNIEDGGVQLADHYQQNTPIGDDPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(1006..1034)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     1629..1649
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     polyA_signal    1749..1883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    1993..2127
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(2429..3142,3143..3145,3146..3148))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2432..3142,3143..3145,3146..3148))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2474..2495)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(4371..4558,4559..4570)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(4604..4620)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4604..4620)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(4657..4677)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4657..4675)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4696..4712)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4709..4731)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4720..4736
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4744..4750,4751..4768,4769..4774))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4789..4810
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4927..4944)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5098..5686)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5178..5197)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5857..6648,6649..6717))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6480..6499
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6718..6822)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6985..7006)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(7459..7470,7471..7633)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     8006..8022
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8042..8062
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ccggtcaaca tggagcaaaa gctgatctcg gaggaggacc tgggcggctc aggcaagaag
       61 aacagggcct tcctgaagtg ggctggcgga aagtacccac tgctggacga catcaagagg
      121 cacctgccca agggcgagtg cctggtcgag cctttcgtgg gagccggaag cgtgttcctg
      181 aacaccgact tcagccgcta catcctggcc gacatcaaca gcgacctgat cagcctgtac
      241 aacatcgtga agatgaggac cgacgagtac gtgcaggccg ctcgcgagct gttcgtgccc
      301 gagacaaact gcgccgaggt gtactaccag ttcagagaag agttcaacaa gagccaggac
      361 cccttccgca gggccgtgct gttcctgtac ctgaacagat acggatacaa cggcgcctgc
      421 agatacaacc tgaggggcga gttcaacgtg cccttcggaa gatacaagaa gccctacttc
      481 cccgaggccg agctgtacca cttcgccgag aaggcccaga acgcattctt ctactgcgag
      541 agctacgccg acagcatggc cagggccgac gacgcctctg tggtgtactg cgaccctccc
      601 tacgcccctc tgagcgccac cgccaacttc accgcctacc acaccaacag cttcaccctg
      661 gagcagcagg cccacctggc cgagatcgct gagggactgg tcgagaggca catccccgtg
      721 ctgatctcca accacgacac catgctgacc agagagtggt atcagagggc caagctgcac
      781 gtggtcaaag tgcgcagatc catcagcagc aacggcggaa ccagaaagaa ggtggacgag
      841 ctgctggccc tgtacaagcc cggcgtggtg tcccctgcca agaaggctag cctgagcggt
      901 ggaggcggtt caggcggagg tggctctggc ggtggcggat cgggaggcgg tggaagtgca
      961 gccgcgacta gtaccatgag taaaggagaa gaacttttca ctggagttgt cccaattctt
     1021 gttgaattag atggtgatgt taatgggtac aaattttctg tcagtggaga gggtgaaggt
     1081 gatgcaacat acggaaaact tacccttaaa tttatttgca ctactggaaa actacctgtt
     1141 ccatggccaa cacttgtcac tactctcact tatggtgttc aatgcttttc aagatatcca
     1201 gatcatatga agcggcacga cttcttcaag agcgccatgc ctgagggata cgtgcaggag
     1261 aggaccatct tcttcaagga cgacgggaac tacaagacac gtgctgaagt caagtttgag
     1321 ggagacaccc tcgtcaacag gatcgagctt aagggaatcg atttcaagga ggacggaaac
     1381 atcctcggcc acaagttgga atacaactac aactcccaca acgtatacat catggccgac
     1441 aagcaaaaga acggcatcaa agccaacttc aagacccgcc acaacatcga agacggcggc
     1501 gtgcaactcg ctgatcatta tcaacaaaat actccaattg gcgatgaccc tgtcctttta
     1561 ccagacaacc attacctgtc cacacaatct gccctttcga aagatcccaa cgaaaagaga
     1621 gaccacatgg tccttcttga gtttgtaacg gctgctggga ttacacatgg catggatgaa
     1681 ctatacaaac cacctcccaa gaggcccagg ctggactaat ctagaactat agtgagtcgt
     1741 attacgtaga tccagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa
     1801 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     1861 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     1921 agggggaggt gtgggaggtt ttttaattcg cggccgcggc gccaatgcat tgggcccggt
     1981 accatcgatg atgatccaga catgataaga tacattgatg agtttggaca aaccacaact
     2041 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     2101 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     2161 gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg
     2221 gctgattatg atcctctaga tcagatctct tgtttattgc agcttataat ggttacaaat
     2281 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     2341 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctacgtaata cgactcacta
     2401 tagttctaga ggctcgagag gggccgcttt acttgtacag ctcgtccatg ccgagagtga
     2461 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     2521 tgctcagggc ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     2581 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     2641 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     2701 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     2761 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     2821 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     2881 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     2941 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     3001 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     3061 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     3121 tgaacagctc ctcgcccttg ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct
     3181 cctgggtcac tggcttacta atggagtctt tatgtatgag gactcttatc aattgttctt
     3241 ctataaaggt ctgcagtgtt tctgttcgtc ccctacatgg acacccagag cctcctaaat
     3301 acaggagccc tgataactgc acaagtgctc agattccagc agggtggaaa atgagataaa
     3361 gtgtgcagat ggggaggggg acgtgaatga gagatttgag ggatgaaaag gatggatgaa
     3421 cgcattgaaa atagcccctt tcacaaaata ataccagtaa attgtcatat aattaatgac
     3481 cagaccttta cctgtaaatg atgtgatgtt catatacaga agaatgccaa caatttacag
     3541 atgattttac aaattcttaa ttttcctttt ctaaatggat ttgttcacac attatctctt
     3601 caaagcaatt gaattttctg gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa
     3661 tttaatgtga catggaatga agcaaacagc agcctaagac aggatggcag gaaaaatgtc
     3721 acatgtttaa aataagagtg aaaccaacga cctggactta tagagactgt gtgtccttag
     3781 gcaaagctct tgtacaaaca gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg
     3841 ttaaatgtgt gcttggatgt atcataaaaa agaatcggtt ctctgtattt gacattttct
     3901 gtaatttatc taaagttaca actggctatg cctgattttt atcactattg aatcaatcat
     3961 tttaaagaat gttaagtctg ctgatttgga gagctcattt acatttattt tgaatgtctc
     4021 ttatttaaca caactgattt aagctttaga tctgcgaaga tacggccacg ggtgctcttg
     4081 atcctgtggc tgattttgga ctgtgctgct cgcagctgct gatgaatcac atacttcctc
     4141 cattttcttc cactgattga ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc
     4201 attgtggtaa tagatgtgac atgacgtcac ttccaaagga ccaatgaaca tgtctgacca
     4261 atttcatata atgtgaaaac gattttcata ggcagaataa ataacattta aattaaactg
     4321 ggcatcagcg caattcaatt ggtttggtaa tagcaaggga aaatagaatg aagtgatctc
     4381 caaaaaataa gtactttttg actgtaaata aaattgtaag gagtaaaaag tacttttttt
     4441 tctaaaaaaa tgtaattaag taaaagtaaa agtattgatt tttaattgta ctcaagtaaa
     4501 gtaaaaatcc ccaaaaataa tacttaagta cagtaatcaa gtaaaattac tcaagtactt
     4561 tacacctctg gttcttgacc ccctaccttc agcaagccca gcagatccac tagttctaga
     4621 gcggccgcca ccgcggtgga gctccagctt ttgttccctt tagtgagggt taattgcgcg
     4681 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4741 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4801 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4861 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4921 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4981 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     5041 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     5101 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5161 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5221 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5281 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5341 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5401 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5461 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5521 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5581 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5641 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5701 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggca ttttggtcat
     5761 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5821 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5881 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5941 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     6001 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     6061 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6121 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6181 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6241 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6301 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6361 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6421 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6481 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6541 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6601 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6661 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6721 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6781 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6841 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6901 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6961 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     7021 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7081 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7141 gggagccccc gatttagagc ttgacgggga aaggctggct aagaactcat cagcctcccc
     7201 ggtccatcta cccacgtacc aatgcaccaa ttggccacaa tgacggctac tacatggtgc
     7261 cattccttcc tctttatagg aatggagact acctcctgtc caacaatgct cttggatacg
     7321 agtacgccta cctgttggac ccaggtcatt gcacaacacc agaaatgccc tctgatctgc
     7381 aaaagacgtg aatatctgtt cagacaccca tatccactct gttccacaca ggtcagaggt
     7441 ttgtccagga gttcttgaca gaggtgtaaa aagtactcaa aaattttact caagtgaaag
     7501 tacaagtact tagggaaaat tttactcaat taaaagtaaa agtatctggc tagaatctta
     7561 cttgagtaaa agtaaaaaag tactccatta aaattgtact tgagtattaa ggaagtaaaa
     7621 gtaaaagcaa gaaagaaaac tagagattct tgtttaagct tttaatctca aaaaacatta
     7681 aatgaaatgc atacaaggtt ttatcctgct ttagaactgt ttgtatttaa ttatcaaact
     7741 ataagacaga caatctaatg ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa
     7801 cttcagtaga agctgattct caaaattgtt agtgtcaagc ctagctcttt tggggctgaa
     7861 aagcaatcct gcagtgctga aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt
     7921 agtcttgata gagaggctgc aaatagcagg aaacgtgagc agagactccc tggtgtctga
     7981 aacacaggcc agatgggccc tcgagcagga aacagctatg accatgatta cgccaagcta
     8041 tcaactttgt atagaaaagt tggctccgaa ttcgccctta aaactcgaga ccagcaaagt
     8101 tctagaattt gtcgaaacat ttatgttata tatttcctga aaaaaattct gagtaagttc
     8161 ttaagtgtta ttgccagcaa cataaacaac agacggcaaa atgaataaat gataacaaag
     8221 cagtaggctt aaataaacct aatttttata ggctgttctc tacaaccctc aaacagtgat
     8281 tagttttgta cttataaact tgccctttca ttcatatttc aagaaaattg gttcagaaga
     8341 tctggatatt ctagcagttg ttcaagctca tggagggatc agtgacctga ttccacaatg
     8401 actaggccta atccagaaat tagatgactg tcaacataaa aaggcacagc actcactagc
     8461 tgccctatat attttattat attttacata tattatttta tttatttagc tctgagtgct
     8521 gtactttctg gttaaagaaa actgcttaca acagctaacc tgtactacct caggctcagg
     8581 gaatttggaa caggtttgtc tggtttgttt ctttaaccat gcatgcttgt tttcaactat
     8641 ggcaacacag tcacatggga cattacagaa atgatttgtc gatgacatgc gacttttctt
     8701 taataaagcg caaagatccc aaaaagcaaa cttttaacaa aaatcatata attatatttt
     8761 caatccagct ttgtagcaac tttgtgctgc tgttcactca gcaacagata gtcagtataa
     8821 ggtcagtgtg tctcaaagca gtgccatctg tttcacacat tgcgttctat atataagtgt
     8881 gctggttgac acgacactgt ataaggccta ggctaaaaca caaacaatgt agaatgacac
     8941 tgtgtttttt ttgtaaacaa atgttgtttt tggttaaaca tctttgtgaa aacatcctcc
     9001 tgtcatgtat ttgctatatt caaatgttaa acccgtgcag aatagaacat atacaaaaaa
     9061 aaacaacaca acacattttt aaacattatt aaatatcaag tattgctggc agttctgttt
     9121 ctgttttaca gtaccctttg ccacagttct ccgcttttcc tggtccagat tccacaagtc
     9181 tgattcacca atagcaaagc gaataaacaa ccaaagcagc caatcactgc ttgtagactg
     9241 tcctgcgaga ccggcccatt ccagcacatt ctggaaactt cctttatatg ataattataa
     9301 atacatttaa attattgata caaaacatgt aattcctaga acataaccat agcaatcatt
     9361 agttttcagg gtaattatgt atttttagga tttgactgcg gaaagatctg gtcatgtgac
     9421 gtctcatgaa cgtcacggcc ctgggtttct ataaatacag taggactctc gaccatcggc
     9481 agatttttcg aagaagaaga tcagtttcag gagccgtact gttccgtttc aacgcaaata
     9541 ttaacggtaa gagcgaattt cctagtttgt tttcatgcca ttctttaaaa ccatagcgta
     9601 ttactttaat tatagtaaac tttcgctttc tttattacaa gagacgtttt gtgttgattc
     9661 tccgcggaca ttttcggtca gacaatcaga aaatgaccgc ggaggaccag taacttgcat
     9721 tacacgtaag ttaaatcttc gtgtattaaa atggttaggt tgttaacgtc aaataggtta
     9781 ccgtgtttgc gtgtgatcag gttggttttg ttagattttt gtcagtattt ttaatttatt
     9841 tgttttagtt tatttatttt ttttgctgaa tcatagtttg tgaacaaaga acccggatgt
     9901 tacatacagt acagccgcca tgttacagag agttataact taatcatttt aaaaataatt
     9961 ttgccttact tttagtttgt catgttgaga aatgaggaaa tgttaaaatg aggaaatatc
    10021 caattaattt aatatatcaa aataatccat gattacaatg cactgaactg gagaaaatta
    10081 agatgttttc tagtgtcatg aaacaaatgt aagagatgta cattgtagat gttttatgtc
    10141 aagaattggc tagttgatgc agcatactgg cgatactcag ttgtaataac agtaacgtta
    10201 catgttaata gactactgag tatgctgttc tgtctatgta tgctctgtaa gctacgagaa
    10261 ggactttttt aaacagtaaa gggtgcaata tttttacaaa ttgaattaaa taaaggctgt
    10321 ctattaagta atatgcttga tatttttctt acttgatcgg aaataagaaa aaatataaac
    10381 gttgttgctc taaaaatcct agttcagttt agccaaccac aaataccttt ttgttcctcc
    10441 aacagttttt ttttcttctc tataatattt ggcagtctat agtactccaa atgtttcccc
    10501 acagtctaac taattggtac agccaaaatc atgacactta ttgcaataat aattttggtt
    10561 cattggcatt gttgatagcc tgtgccacta atatggtcga ttgatcatgc ttcaggaaga
    10621 aaactatatt gtttgatgta agattattaa atcttcacct gcttccatta caaactattc
    10681 ccatcttatt gaattctggt atgtcttaaa ggattagttc acttcccaaa tcaaaattta
    10741 cttagccttt tttcatccat gatccctttt tttcatcatt aatgaagaaa ttgtttttga
    10801 aaaagtttca agattttttt ctctatattg tggagcttgt tagtttaaaa ttccaaaatg
    10861 caatatgtgg cttcaaatgg ttctaaatga tcccagtcaa ggaataacag tcttatctaa
    10921 tgaaaccatt agacctttaa aaataaaaaa aataaaagta tttattttta aatgactgag
    10981 tgattaagtt gaatttcagc gtttccttac tgtgtagaag tccttcctta ctggccccac
    11041 cctttggttc tctgccaatc tgctacctaa tgtaatgttg tggaacatta ttattcttta
    11101 tttcttaatt ttttattttt tattttaaaa caatgtaaac tgcacagatg tgcagtttgt
    11161 ttaaaatggc caatgctttg gaaatgcatg acataattag atttcatgat gcacaaagcc
    11221 aaatctcaga gcttgtgcaa aatgagctat catttcacta ggtaagaccc taaattttca
    11281 tataggatca tttggacaat tttgctgcag gtaaaatgca ttctatagtc cactgtcagc
    11341 cattgttttg gatagtattt atttttctct acaagtatag tcaatagttt tctattattt
    11401 taaaggtttg taacatttaa gggtgaccaa atgcaaagta aaatttcatt ttcgggtgaa
    11461 ctatctcgtt taacatggga gaagtgcaaa acatacatta ttggctagaa cattgtagta
    11521 ttttttaaat ggaaatgtgt gattgctaat cttactttga atttgtttac agggatcca
//
