LOCUS       sequence-170483-        9215 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  homology donor for AAT locus with piggyBac-flanked 
            DsRedEx_T2A_puroTK cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 9215)
  AUTHORS   Eggenschwiler R, Moslem M, Fraguas MS, Galla M, Papp O, Naujock M, 
            Fonfara I, Gensch I, Wahner A, Beh-Pajooh A, Mussolino C, Tauscher 
            M, Steinemann D, Wegner F, Petri S, Schambach A, Charpentier E, 
            Cathomen T, Cantz T
  TITLE     Improved bi-allelic modification of a transcriptionally silent locus
            in patient-derived iPSC by Cas9 nickase.
  JOURNAL   Sci Rep. 2016 Dec 2;6:38198. doi: 10.1038/srep38198.
  PUBMED    27910942
REFERENCE   2  (bases 1 to 9215)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-12-2"; volume: "6:38198. doi"; pages: " 
            10.1038/srep38198"
COMMENT     Sequence Label: AAT-PB-CD2APtk
FEATURES             Location/Qualifiers
     source          1..9215
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     313..335
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     397..416
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      461..470
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             467..1141
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGSFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERAEGRHH
                     LFL"
     primer_bind     complement(597..615)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(647..667)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     865..884
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1059..1082
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             1157..1210
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             1214..1810
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1214..1233)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     1710..1730
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             1817..2812
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWQV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQGGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRPMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     primer_bind     complement(2834..2851)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2840..3064
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(3150..3174)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     repeat_region   3356..3418
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     4441..4457
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4491..4507)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4492..4508)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(4537..4557)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(4537..4555)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4576..4592)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4576..4592)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4589..4611)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4600..4616
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4624..4630,4631..4648,4649..4654))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4669..4690
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4807..4824)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4978..5566)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5058..5077)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5737..6528,6529..6597))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6360..6379
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6598..6702)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(6728..7183)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6865..6886)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7077..7096
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7310..7332
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7324..7341
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7325..7341
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7351..7370
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7351..7369
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   8405..8439
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        8729..9108
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gggaggggcg cggcggcccc cggagcgccg gcggctgtcg aggcgcggcg agccgcagcc
       61 attgcctttt atggtaatcg tgcgagaggg cgcagggact tcctttgtcc caaatctgtg
      121 cggagccgaa atctgggagg cgccgccgca ccccctctag cgggcgcggg gcgaagcggt
      181 gcggcgccgg caggaaggaa atgggcgggg agggccttcg tgcgtcgccg cgccgccgtc
      241 cccttctccc tctccagcct cggggctgtc cgcgggggga cggctgcctt cgggggggac
      301 ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc tctgctaacc
      361 atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt attgtgctgt
      421 ctcatcattt tggcaaagaa ttcctcgaga tccaccggtc gccaccatgg cctcctccga
      481 ggacgtcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca
      541 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa
      601 gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt
      661 ccagtacggc tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct
      721 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt
      781 gaccgtgacc caggactcct ccctgcagga cggctccttc atctacaagg tgaagttcat
      841 cggcgtgaac ttcccctccg acggccccgt aatgcagaag aagactatgg gctgggaggc
      901 ctccaccgag cgcctgtacc cccgcgacgg cgtgctgaag ggcgagatcc acaaggccct
      961 gaagctgaag gacggcggcc actacctcgt ggagttcaag tccatctaca tggccaagaa
     1021 gcccgtgcag ctgcccggct actactacgt ggactccaag ctggacatca cctcccacaa
     1081 cgaggactac accatcgtgg agcagtacga gcgcgccgag ggccgccacc acctgttcct
     1141 gtacaagggc tccggagagg gccggggctc tctgctgacc tgtggcgacg tggaggagaa
     1201 ccccggcccc tccatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     1261 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     1321 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     1381 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgcgg tggcggtctg
     1441 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     1501 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     1561 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     1621 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     1681 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     1741 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     1801 gcccggtgcc ggatccatgc ccacgctact gcgggtttat atagacggtc ctcacgggat
     1861 ggggaaaacc accaccacgc aactgctggt ggccctgggt tcgcgcgacg atatcgtcta
     1921 cgtacccgag ccgatgactt actggcaggt gctgggggct tccgagacaa tcgcgaacat
     1981 ctacaccaca caacaccgcc tcgaccaggg tgagatatcg gccggggacg cggcggtggt
     2041 aatgacaagc gcccagataa caatgggcat gccttatgcc gtgaccgacg ccgttctggc
     2101 tcctcatatc gggggggagg ctgggagctc acatgccccg cccccggccc tcaccctcat
     2161 cttcgaccgc catcccatcg ccgccctcct gtgctacccg gccgcgcgat accttatggg
     2221 cagcatgacc ccccaggccg tgctggcgtt cgtggccctc atcccgccga ccttgcccgg
     2281 cacaaacatc gtgttggggg cccttccgga ggacagacac atcgaccgcc tggccaaacg
     2341 ccagcgcccc ggcgagcggc ttgacctggc tatgctggcc gcgattcgcc gcgtttacgg
     2401 gctgcttgcc aatacggtgc ggtatctgca gggcggcggg tcgtggcggg aggattgggg
     2461 acagctttcg gggacggccg tgccgcccca gggtgccgag ccccagagca acgcgggccc
     2521 acgaccccat atcggggaca cgttatttac cctgtttcgg gcccccgagt tgctggcccc
     2581 caacggcgac ctgtacaacg tgtttgcctg ggccttggac gtcttggcca aacgcctccg
     2641 tcccatgcac gtctttatcc tggattacga ccaatcgccc gccggctgcc gggacgccct
     2701 gctgcaactt acctccggga tggtccagac ccacgtcacc acccccggct ccataccgac
     2761 gatctgcgac ctggcgcgca cgtttgcccg ggagatgggg gaggctaact gagctctaga
     2821 gctcgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc
     2881 cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag
     2941 gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag
     3001 gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct
     3061 atggcttctg aggcggaaag aaccagctgg ggctcgagat ccactagttc tagcctcgag
     3121 gctagagcgg ccgcactcga gatatctaga cccagctttc ttgtacaaag tggtactagt
     3181 taaaagtttt gttactttat agaagaaatt ttgagttttt gttttttttt aataaataaa
     3241 taaacataaa taaattgttt gttgaattta ttattagtat gtaagtgtaa atataataaa
     3301 acttaatatc tattcaaatt aataaataaa cctcgatata cagaccgata aaacacatgc
     3361 gtcaatttta cgcatgatta tctttaacgt acgtcacaat atgattatct ttctagggtt
     3421 aaccatcgac gagaaaggga ctgaagctgc tggggccatg tttttagagg ccatacccat
     3481 gtctatcccc cccgaggtca agttcaacaa accctttgtc ttcttaatga ttgaacaaaa
     3541 taccaagtct cccctcttca tgggaaaagt ggtgaatccc acccaaaaat aactgcctct
     3601 cgctcctcaa cccctcccct ccatccctgg ccccctccct ggatgacatt aaagaagggt
     3661 tgagctggtc cctgcctgca tgtgactgta aatccctccc atgttttctc tgagtctccc
     3721 tttgcctgct gaggctgtat gtgggctcca ggtaacagtg ctgtcttcgg gccccctgaa
     3781 ctgtgttcat ggagcatctg gctgggtagg cacatgctgg gcttgaatcc aggggggact
     3841 gaatcctcag cttacggacc tgggcccatc tgtttctgga gggctccagt cttccttgtc
     3901 ctgtctttga gtccccaaga aggaatcaca ggggaggaac cagataccag ccatgacccc
     3961 aggctccacc aagcatcttc atgtccccct gctcatcccc cactcccccc cacccagagt
     4021 tgctcatcct gccagggctg gctgtgccca ccccaaggct gccctcctgg ggaccccaga
     4081 actgcctgat cgtgccgtgg cccagttttg tggcatctgc agcaacacaa gagagaggac
     4141 aatgtcctcc tcttgacccg ctgtcaccta accagactcg ggccctgcac ctctcaggca
     4201 cttctggaaa atgactgagg cagattcttc ctgaagccca ttctccatgg ggcaacaagg
     4261 acacctattc tgtccttgtc cttccatcgc tgccccagaa agcctcacat atctccgttt
     4321 agaatcaggt cccttctccc cagatgaaga ggagggtctc tgctttgttt tctctatctc
     4381 ctcctcagac ttgaccaggc ccagcaggcc ccagaagacc attaattaag gccgggccgc
     4441 tctagaacta gtggatcccc cgggctgcag gaattcgata tcaagcttat cgataccgtc
     4501 gacctcgagg gggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg
     4561 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
     4621 acacaacata cgagccggga gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
     4681 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
     4741 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
     4801 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
     4861 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4921 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4981 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     5041 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     5101 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     5161 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     5221 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     5281 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     5341 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     5401 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5461 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5521 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5581 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5641 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5701 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
     5761 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
     5821 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
     5881 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
     5941 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
     6001 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
     6061 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
     6121 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
     6181 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
     6241 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
     6301 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
     6361 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
     6421 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
     6481 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
     6541 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
     6601 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
     6661 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
     6721 gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6781 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6841 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6901 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6961 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7021 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7081 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7141 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc gccattcagg
     7201 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7261 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7321 cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag
     7381 ctcgtttaaa cggcgcgcct ctgcacgaca ggtctgccag cttacattta cccaaactgt
     7441 ccattactgg aacctatgat ctgaagagcg tcctgggtca actgggcatc actaaggtct
     7501 tcagcaatgg ggctgacctc tccggggtca cagaggaggc acccctgaag ctctccaagg
     7561 tgagatcacc ctgacgacct tgttgcaccc tggtatctgt agggaagaat gtgtgggggc
     7621 tgcagctctg tcctgaggct gaggaagggg ccgagggaaa caaatgaaga cccaggctga
     7681 gctcctgaag atgcccgtga ttcactgaca cgggacgtgg tcaaacagca aagccaggca
     7741 ggggactgct gtgcagctgg cactttcggg gcctcccttg aggttgtgtc actgaccctg
     7801 aatttcaact ttgcccaaga ccttctagac attgggcctt gatttatcca tactgacaca
     7861 gaaaggtttg ggctaagttg tttcaaagga atttctgact ccttcgatct gtgagatttg
     7921 gtgtctgaat taatgaatga tttcagctaa agatgacact tattttggaa aactaaaggc
     7981 gaccaatgaa caactgcagt tccatgaatg gctgcattat cttggggtct gggcactgtg
     8041 aaggtcactg ccagggtccg tgtcctcaag gagcttcaag ccgtgtacta gaaaggagag
     8101 agccctggag gcagacgtgg agtgacgatg ctcttccctg ttctgagttg tgggtgcacc
     8161 tgagcagggg gagaggcgct tgtcaggaag atggacagag gggagccagc cccatcagcc
     8221 aaagccttga ggaggagcaa ggcctatgtg acagggaggg agaggatgtg cagggccagg
     8281 gccgtccagg gggagtgagc gcttcctggg aggtgtccac gtgagccttg ctcgaggcct
     8341 gggatcagcc ttacaacgtg tctctgcttc tctcccctcc aggccgtgca taaggctgtg
     8401 ttaaccctag aaagatagtc tgcgtaaaat tgacgcatgc attcttgaaa tattgctctc
     8461 tctttctaaa tagcgcgaat ccgtcgctgt gcatttagga catctcagtc gccgcttgga
     8521 gctcccgtga ggcgtgcttg tcaatgcggt aagtgtcact gattttgaac tataacgacc
     8581 gcgtgagtca aaatgacgca tgattatctt ttacgtgact tttaagattt aactcatacg
     8641 ataattatat tgttatttca tgttctactt acgtgataac ttattatata tatattttct
     8701 tgttatagat atcaactaga atgctagcga cattgattat tgactagtta ttaatagtaa
     8761 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     8821 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     8881 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     8941 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     9001 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     9061 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt cgaggtgagc
     9121 cccacgttct gcttcactct ccccatctcc cccccctccc cacccccaat tttgtattta
     9181 tttatttttt aattattttg tgcagcgatg ggggg
//
