LOCUS       sequence-170491-        5474 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Protein expression in mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyBADC
FEATURES             Location/Qualifiers
     source          1..5474
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1014..1602
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1503..1522
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3717..4595)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        4622..4906
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     4829..4848
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     CDS             4993..5010
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      5069..5115
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gtgcaggtcg ccgtggacga accggggttc gcggccggcc agcagcgtgt ccacgtccgg
       61 cagccagtcc tccaggcggt ccagcagccg gggcgagagg tagccccacc cgcggtggtc
      121 ctcgacggtc gccgcgcggc gttcccgcag cagttccggg aagacctcgg aatggggggt
      181 gagcacggtg ttcccggtca gcggcaccct gtgcagccgg ccgagcaccc ggccgagttc
      241 gcgggccagg gcgagcagcg cgttccggtc ggtcgtgccg tccatcgcgg accgccaggt
      301 ggtgccggtc atccggctca tcaccaggta gggccacggc caggctccgg tgccgggccg
      361 cagctcgccg cggccgagga ggcggggcac cggcaccggg gcgtccgcca ggaccgcgta
      421 cgcctccgac tccgacgcga ggctctccgg accgcaccag tgctcgccga acagcttgat
      481 caccgggccg ggctcgccga ccagtacggg gttggtgctc tcgccgggca cccgcagcac
      541 cggcggcacc ggcagcccga gctcctccag ggctcggcgg gccagcggct cccagaattc
      601 ctggtcgttc cgcaggctcg cgtaggaatc atccgaatca atacggtcga gaagtaacag
      661 ggattcttgt gtcacagcgg acctctattc acagggtacg ggccggctta attccgcacg
      721 gccggtcgcg acacggcctg tccgcaccgc ggatcaggcg ttgacgatga cgggctggtc
      781 ggccacgtcg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
      841 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
      901 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
      961 catgctctag ttccatgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     1021 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     1081 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     1141 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     1201 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     1261 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     1321 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     1381 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga ggggagaaag
     1441 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     1501 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     1561 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     1621 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     1681 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     1741 cgaacgaccg agcgcaacgc gtgcggccgc acgcgtgagc ccaccagctc cgtaagttcg
     1801 ggcgctgtgt ggctcgtacc cgcgcattca ggcggcaggg ggtctaacgg gtctaaggcg
     1861 gcgtgtacgg ccgccacagc ggctctcagc ggcccggaaa cgtcctcgaa acgacgcatg
     1921 tgttcctcct ggttggtaca ggtggttggg ggtgctcggc tgtcgctggt gttccaccac
     1981 cagggctcga cgggagagcg ggggagtgtg cagttgtggg gtggcccctc agcgaaatat
     2041 ctgacttgga gctcgtgtcg gaccatacac cggtgattaa tcgtggtcta ctaccaagcg
     2101 tgagccacgt cgccgacgaa tttgagcagc tctggctgcc gtactggccg ctggcaagcg
     2161 acgatctgct cgaggggatc taccgccaaa gccgcgcgtc ggccctaggc cgccggtaca
     2221 tcgaggcgaa cccaacagcg ctggcaaacc tgctggtcgt ggacgtagac catccagacg
     2281 cagcgctccg agcgctcagc gcccgggggt cccatccgct gcccaacgcg atcgtgggca
     2341 atcgcgccaa cggccacgca cacgcagtgt gggcactcaa cgcccctgtt ccacgcaccg
     2401 aatacgcgcg gcgtaagccg ctcgcataca tggcggcgtg cgccgaaggc cttcggcgcg
     2461 ccgtcgatgg cgaccgcagt tactcaggcc tcatgaccaa aaaccccggc cacatcgcct
     2521 gggaaacgga atggctccac tcagatctct acacactcag ccacatcgag gccgagctcg
     2581 gcgcgaacat gccaccgccg cgctggcgtc agcagaccac gtacaaagcg gctccgacgc
     2641 cgctagggcg gaattgcgca ctgttcgatt ccgtcaggtt gtgggcctat cgtcccgccc
     2701 tcatgcggat ctacctgccg acccggaacg tggacggact cggccgcgcg atctatgccg
     2761 agtgccacgc gcgaaacgcc gaatttccgt gcaacgacgt gtgtcccgga ccgctaccgg
     2821 acagcgaggt ccgcgccatc gccaacagca tttggcgttg gatcacaacc aagtcgcgca
     2881 tttgggcgga cgggatcgtg gtctacgagg ccacactcag tgcgcgccag tcggccatct
     2941 cgcggaaggg cgcagcagcg cgcacggcgg cgagcacagt tgcgcggcgc gcaaagtccg
     3001 cgtcagccat gcatggaggc attgctatga gcgacggcta cagcgacggc tacaaccggc
     3061 agccgactgt ccgcaaaaag cggcgcgtga ccgccgccga aggcgctcga atcaccggac
     3121 tatccgaacg ccacgtcgtc cggctcgtgg cgcaggaacg cagcgagtgg ctcgccgagc
     3181 aggctgcacg ccgcgaacgc atccgcgcct atcacgacga cgagggccac tcttggccgc
     3241 aaacggccaa acatttcggg ctgcatctgg acaccgttaa gcgactcggc tatcgggcga
     3301 ggaaagagcg tgcggcagaa caggaagcgg ctcaaaaggc ccacaacgaa gccgacaatc
     3361 caccgctgtt ctaacgcaat tggggagcgg gtgtcgcggg ggttccgtgg ggggttccgt
     3421 tgcaacgggt cggacaggta aaagtcctgg tagacgctag ttttctggtt tgggccatgc
     3481 ctgtctcgtt gcgtgtttcg ttgcgcccgt tttgaatacc agccagacga gacggggttc
     3541 tacgaatctt ggtcgatacc aagccatttc cgctgaatat cggggagctc accgccagaa
     3601 tcggtggttg tggtgatgta cgtggcgaac tccgttgtag tgcctgtggt ggcatccgtg
     3661 gcgcggccgc ggtaccagat ctttaaatct agataaagaa gtgacgcggt cctagattat
     3721 gacaacttga cggctacatc attcactttt tcttcacaac cggcacggaa ctcgctcggg
     3781 ctggccccgg tgcatttttt aaatacccgc gagaaataga gttgatcgtc aaaaccaaca
     3841 ttgcgaccga cggtggcgat aggcatccgg gtggtgctca aaagcagctt cgcctggctg
     3901 atacgttggt cctcgcgcca gcttaagacg ctaatcccta actgctggcg gaaaagatgt
     3961 gacagacgcg acggcgacaa gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg
     4021 tctgccaggt gatcgctgat gtactgacaa gcctcgcgta cccgattatc catcggtgga
     4081 tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca attgctcaag cagatttatc
     4141 gccagcagct ccgaatagcg cccttcccct tgcccggcgt taatgatttg cccaaacagg
     4201 tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga accccgtatt ggcaaatatt
     4261 gacggccagt taagccattc atgccagtag gcgcgcggac gaaagtaaac ccactggtga
     4321 taccattcgc gagcctccgg atgacgaccg tagtgatgaa tctctcctgg cgggaacagc
     4381 aaaatatcac ccggtcggca aacaaattct cgtccctgat ttttcaccac cccctgaccg
     4441 cgaatggtga gattgagaat ataacctttc attcccagcg gtcggtcgat aaaaaaatcg
     4501 agataaccgt tggcctcaat cggcgttaaa cccgccacca gatgggcatt aaacgagtat
     4561 cccggcagca ggggatcatt ttgcgcttca gccatacttt tcatactccc gccattcaga
     4621 gaagaaacca attgtccata ttgcatcaga cattgccgtc actgcgtctt ttactggctc
     4681 ttctcgctaa ccaaaccggt aaccccgctt attaaaagca ttctgtaaca aagcgggacc
     4741 aaagccatga caaaaacgcg taacaaaagt gtctataatc acggcagaaa agtccacatt
     4801 gattatttgc acggcgtcac actttgctat gccatagcat ttttatccat aagattagcg
     4861 gatcctacct gacgcttttt atcgcaactc tctactgttt ctccataccc gttttttggg
     4921 ctaacaggag gaattaaccc catgggctcg gccggtaccg gatccgaatt cgatatcgct
     4981 agccaagctt cgcaccacca ccaccaccac tgataacagc ttatcgatgt cgacgtagtt
     5041 aactagcgta cgatcgactg ccaggcatca aataaaacga aaggctcagt cgaaagactg
     5101 ggcctttcgt tttatgccat catggccgcg gtgatcagct agagggggcg tcaggcgccg
     5161 ggggcggtgt ccggcggccc ccagaggaac tgcgccagtt cctccggatc ggtgaagccg
     5221 gagagatcca gcggggtctc ctcgaacacc tcgaagtcgt gcaggaaggt gaaggcgagc
     5281 agttcgcggg cgaagtcctc ggtccgcttc cactgcgccc cgtcgagcag cgcggccagg
     5341 atctcgcggt cgccccggaa ggcgttgaga tgcagttgca ccaggctgta gcgggagtct
     5401 cccgcataga cgtcggtgaa gtcgacgatc ccggtgacct cggtcgcggc caggtccacg
     5461 aagatgttgg tccc
//
