LOCUS       sequence-170498-        6616 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry fluorescence protein expression using M. smegmatis.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6616)
  AUTHORS   Magana Vergara C, Kallenberg CJL, Rogasch M, Hubner CG, Song YH
  TITLE     A versatile vector for mycobacterial protein production with a 
            functional minimized acetamidase regulon.
  JOURNAL   Protein Sci. 2017 Aug 31. doi: 10.1002/pro.3288.
  PUBMED    28857325
REFERENCE   2  (bases 1 to 6616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2017 Aug 31. doi: 10.1002/pro.3288."
COMMENT     Sequence Label: pMyCA-mCherry
FEATURES             Location/Qualifiers
     source          1..6616
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3627..4215)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3707..3726)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(5588..5634)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(5656..6366)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5934..5953)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     6203..6221
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     CDS             complement(6367..6387)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(6400..6417)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 cctcggctac cgcgtccacc aggcggttgc gcagcgtccg cccccgcggg gtcaacgcca
       61 cgagcacccg ccggcggtca tgcagatcgg cagtccggta gatgatccct tgcgacacaa
      121 ggcgatcgac ggttctggtg agagtcggac ccgtgatcag cgtggcctgc gcgagatcgc
      181 tcatgggcac ggtgcgccgt ccggccaact cgtcgagcac gcgccagtcc tcgatcttga
      241 gcgacgagtc cgcgagcacg cgttcgatcg ccgactccgc gaggtgggtc agacgcacca
      301 acggcagcag gaggttgggg cggcgctgct gtccggcggc catgttgggt ctcctcgtgt
      361 cgacggcggg gtctgttcag aatgaattat ttctgctaga aacctttgaa gcgcagacta
      421 cacgggatcg acggtgatct ggagtgcagg acggcgaggt tgagttccgc gtcgggctgg
      481 tgattccgct tcaagggcca gcgggaatct tcgcgccttc gtgcgaggcc gtggccgagc
      541 tcgcggccaa ggaggtcaac gaccgaggcg gcctgcaggg ccgcaaggtc accatcgagg
      601 tgctcgacgg cggagcgccc ggcgacgacg tcgcccggac cgtcgccgac cggttgcgag
      661 gtcacggtct cgacgccgtg accggctggc acatctcggc cgtgcgcaac cgcatctccc
      721 cggtggtccg cgaccgcatc ccgtacgtct acacctcgtt gtacgagggc ggtgaacgca
      781 caccgggcgt gttctgcaca ggcgagacac cgcagatcca gatcgcgccc gcgctcgcct
      841 ggctgcgcga ccacttcggc atccggtcct ggtgcctggt cggcgatgac tacatctggc
      901 cgcgccgttc cgccgcggcc gcccgcgcgt actgccgaga tctggacctc gagctcagac
      961 gggagatcta cgtcccgtac ggcaccgacg atttccgcgc acctgtccgc aaggccatcg
     1021 cgtcgggggc gcaggccgtg ttgatgctgc tcgtcggcca ggacgccgtg ttgttcaacc
     1081 gcgagttcgc gcgcgccggc gggcacgacc gcatggcgcg gttcagcccg ctgatggagg
     1141 agaacatgct gctggccagc ggcgccggct ccaccgaaaa cctctatgtc gccgcggcgt
     1201 acttcagttc actggccacc gcgggtgcga tggacctgat gggcagctac gtcgcccgct
     1261 acggcgccga cgcgccaccg ctcaacgcga tggccgaatc ctgttacgag ggtctgcttg
     1321 cgctcgaggc catcttccag cgggcccact cccccgagat accggacctg atggcatccg
     1381 cgcacgatgt cgggttcgac gggccccggg gcccgatgtg tatgcgcgac agtcaattcg
     1441 accagcaggt ctacatcgcc agcgccgacg gctacgactt cgacatcctc gacacgctgg
     1501 cgacgctcga cgcttgagac cgcgtcactt ctttatctag atttaaagat ctggtaccgc
     1561 ggccgcgcca cggatgccac cacaggcact acaacggagt tcgccacgta catcaccaca
     1621 accaccgatt ctggcggtga gctccccgat attcagcgga aatggcttgg tatcgaccaa
     1681 gattcgtaga accccgtctc gtctggctgg tattcaaaac gggcgcaacg aaacacgcaa
     1741 cgagacaggc atggcccaaa ccagaaaact agcgtctacc aggactttta cctgtccgac
     1801 ccgttgcaac ggaacccccc acggaacccc cgcgacaccc gctccccaat tgcgttagaa
     1861 cagcggtgga ttgtcggctt cgttgtgggc cttttgagcc gcttcctgtt ctgccgcacg
     1921 ctctttcctc gcccgatagc cgagtcgctt aacggtgtcc agatgcagcc cgaaatgttt
     1981 ggccgtttgc ggccaagagt ggccctcgtc gtcgtgatag gcgcggatgc gttcgcggcg
     2041 tgcagcctgc tcggcgagcc actcgctgcg ttcctgcgcc acgagccgga cgacgtggcg
     2101 ttcggatagt ccggtgattc gagcgccttc ggcggcggtc acgcgccgct ttttgcggac
     2161 agtcggctgc cggttgtagc cgtcgctgta gccgtcgctc atagcaatgc ctccatgcat
     2221 ggctgacgcg gactttgcgc gccgcgcaac tgtgctcgcc gccgtgcgcg ctgctgcgcc
     2281 cttccgcgag atggccgact ggcgcgcact gagtgtggcc tcgtagacca cgatcccgtc
     2341 cgcccaaatg cgcgacttgg ttgtgatcca acgccaaatg ctgttggcga tggcgcggac
     2401 ctcgctgtcc ggtagcggtc cgggacacac gtcgttgcac ggaaattcgg cgtttcgcgc
     2461 gtggcactcg gcatagatcg cgcggccgag tccgtccacg ttccgggtcg gcaggtagat
     2521 ccgcatgagg gcgggacgat aggcccacaa cctgacggaa tcgaacagtg cgcaattccg
     2581 ccctagcggc gtcggagccg ctttgtacgt ggtctgctga cgccagcgcg gcggtggcat
     2641 gttcgcgccg agctcggcct cgatgtggct gagtgtgtag agatctgagt ggagccattc
     2701 cgtttcccag gcgatgtggc cggggttttt ggtcatgagg cctgagtaac tgcggtcgcc
     2761 atcgacggcg cgccgaaggc cttcggcgca cgccgccatg tatgcgagcg gcttacgccg
     2821 cgcgtattcg gtgcgtggaa caggggcgtt gagtgcccac actgcgtgtg cgtggccgtt
     2881 ggcgcgattg cccacgatcg cgttgggcag cggatgggac ccccgggcgc tgagcgctcg
     2941 gagcgctgcg tctggatggt ctacgtccac gaccagcagg tttgccagcg ctgttgggtt
     3001 cgcctcgatg taccggcggc ctagggccga cgcgcggctt tggcggtaga tcccctcgag
     3061 cagatcgtcg cttgccagcg gccagtacgg cagccagagc tgctcaaatt cgtcggcgac
     3121 gtggctcacg cttggtagta gaccacgatt aatcaccggt gtatggtccg acacgagctc
     3181 caagtcagat atttcgctga ggggccaccc cacaactgca cactcccccg ctctcccgtc
     3241 gagccctggt ggtggaacac cagcgacagc cgagcacccc caaccacctg taccaaccag
     3301 gaggaacaca tgcgtcgttt cgaggacgtt tccgggccgc tgagagccgc tgtggcggcc
     3361 gtacacgccg ccttagaccc gttagacccc ctgccgcctg aatgcgcggg tacgagccac
     3421 acagcgcccg aacttacgga gctggtgggc tcacgcgtgc ggccgcacgc gttgcgctcg
     3481 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3541 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3601 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3781 ctgtccgcct ttctcccctc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     3841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     4081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4201 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcatggaact
     4261 agagcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     4321 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     4381 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     4441 acgtggccga ccagcccgtc atcgtcaacg cctgatccgc ggtgcggaca ggccgtgtcg
     4501 cgaccggccg tgcggaatta agccggcccg taccctgtga atagaggtcc gctgtgacac
     4561 aagaatccct gttacttctc gaccgtattg attcggatga ttcctacgcg agcctgcgga
     4621 acgaccagga attctgggag ccgctggccc gccgagccct ggaggagctc gggctgccgg
     4681 tgccgccggt gctgcgggtg cccggcgaga gcaccaaccc cgtactggtc ggcgagcccg
     4741 gcccggtgat caagctgttc ggcgagcact ggtgcggtcc ggagagcctc gcgtcggagt
     4801 cggaggcgta cgcggtcctg gcggacgccc cggtgccggt gccccgcctc ctcggccgcg
     4861 gcgagctgcg gcccggcacc ggagcctggc cgtggcccta cctggtgatg agccggatga
     4921 ccggcaccac ctggcggtcc gcgatggacg gcacgaccga ccggaacgcg ctgctcgccc
     4981 tggcccgcga actcggccgg gtgctcggcc ggctgcacag ggtgccgctg accgggaaca
     5041 ccgtgctcac cccccattcc gaggtcttcc cggaactgct gcgggaacgc cgcgcggcga
     5101 ccgtcgagga ccaccgcggg tggggctacc tctcgccccg gctgctggac cgcctggagg
     5161 actggctgcc ggacgtggac acgctgctgg ccggccgcga accccggttc gtccacggcg
     5221 acctgcacgg gaccaacatc ttcgtggacc tggccgcgac cgaggtcacc gggatcgtcg
     5281 acttcaccga cgtctatgcg ggagactccc gctacagcct ggtgcaactg catctcaacg
     5341 ccttccgggg cgaccgcgag atcctggccg cgctgctcga cggggcgcag tggaagcgga
     5401 ccgaggactt cgcccgcgaa ctgctcgcct tcaccttcct gcacgacttc gaggtgttcg
     5461 aggagacccc gctggatctc tccggcttca ccgatccgga ggaactggcg cagttcctct
     5521 gggggccgcc ggacaccgcc cccggcgcct gacgccccct ctagctgatc accgcggcca
     5581 tgatggcata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta tttgatgcct
     5641 ggcagtcgat cgtcgttact tgtacagctc gtccatgccg ccggtggagt ggcggccctc
     5701 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
     5761 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
     5821 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
     5881 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
     5941 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
     6001 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
     6061 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
     6121 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
     6181 ggcgaagggc agggggccac ccttggtcac cttcagcttg gcggtctggg tgccctcgta
     6241 ggggcggccc tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat
     6301 gtgcaccttg aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct
     6361 caccatgccc tggaagtaca ggttctcgcc ggccgacggg tggtggtggt ggtggtgctt
     6421 catggtggac tccctttctc ttatgtacag gtcacccctt tccattcacc cgaaatatat
     6481 ccagatgaaa ataaaagtca agggtgaacg cccgcgccgg gcgcgggtgg ttacgaagtc
     6541 aaattcgagg tggtgtcgac gagttcgcgc aactggtcga cgtccagccc gcacgattca
     6601 aaagccgcac actcgg
//
