LOCUS       sequence-17055-e         748 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 748)
  AUTHORS   Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P
  TITLE     Nutrient control of glucose homeostasis through a complex of 
            PGC-1alpha and SIRT1.
  JOURNAL   Nature 2005 Mar 3;434(7029):113-8.
  PUBMED    15744310
REFERENCE   2  (bases 1 to 748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005-03-3"; volume: "434(7029)"; pages: "113-8"
COMMENT     Sequence Label: pAd-Track Flag-SIRT1
FEATURES             Location/Qualifiers
     source          1..748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      305..314
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(414..446)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(468..491)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             501..523
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    538..562
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(562..581)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(563..581)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(647..746)
                     /label=CMVd1 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields slightly reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(676..696)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 gcctcgtcct cctcctcgcc ctcgtcgtcg tcgaagtcgt cggccgccct cggctcccgc
       61 cgcaggccgg acccattgtc tccgtccccg gccacggccg tcgccggggc ctcccgctcc
      121 gcgctcgccg ccgcccctgc cgcctcccgc cacagcgccg ccggggccgc ggcgctcgcc
      181 gcctcacacc ccgcggccgc cggcgccact gctgcgctcg gctcgcccgg gctgcgcccg
      241 aggccaggcc cgtctcggcg gggcctcttg cggagcggct cgtccgccgg ctgcgacgcg
      301 gcctccatgg cggccgccgc ggaaggggag ccggcggcct gaagggcgag cgccacctcg
      361 tccgccatct tccaactgcc tctctgtcga cggagctcga attcggatcc gcgacccatt
      421 tgctgtccac cagtcatgct agccatatgc acagatgctc ttcttgcttt atcgtcatcg
      481 tctttgtagt ccatggtata tctccttctt aaagttaaac aaaattattt ctagagggga
      541 attgttatcc gctcacaatt cccctatagt gagtcgtatt aatttcgcgg gatcgagatc
      601 cgagctcggt accagatctc tagcggatct gacggttcac taaaccagct ctgcttatat
      661 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
      721 attttggaaa gtcccgttga ttttggtg
//
