LOCUS       sequence-170553-       11837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLP/Frt dual color reporter for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11837)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 11837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN154
FEATURES             Location/Qualifiers
     source          1..11837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(387..420)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     456..472
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     457..473
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             3048..3059
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     rep_origin      complement(4695..5283)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4775..4794)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5454..6245,6246..6314))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6077..6096
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6315..6419)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6487..6505
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6543..6565)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(9609..9629)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     10377..10405
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    complement(10452..10485)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctcctccgtc ttcaaaattc attacgcgtt cccacttaaa accctccggg aatgaaagtt
       61 tcaaataatc tggtatatct gccggatgtt taacataagc tttacttcca tacatgaact
      121 gtggagagag gatgtcccag gcgaatggta atggtccgcc tttagttacc tgaaaattta
      181 aataatcagg gttagttagt atatatatgt ttaaacttac ctttagtttt gcggtttgtg
      241 ttccctcata tggtcggcct tctccttcac cttcaatttc aaactcatgc ccattgacag
      301 atccctccat atgcaccttg aaacgcataa actctttaat aattgccatg ttatcttctt
      361 caccctttga gaccattttt ggatccgaag ttcctatact ttctagagaa taggaacttc
      421 gtaagcctgc ttttttgtac gcggccgcct gcaggtcgag gtcgacggta tcgataagct
      481 tgatatcgaa ttcctgcagc ccgggggatc cgatgaggat tttcgaagtt ttttagatgc
      541 actagaacaa agcgtgttgg cttcctctga gcccgctttc cttatatacc cgcattctgc
      601 agccttacag aatgttctag aaggtcctag atgcattcgt ttgaaaatac tcccggtggg
      661 tgcaaagaga cgcagacgga aaatgtatct gggtctcttt attgtgtaca ctacttttcc
      721 atgtaccgaa tgtgagtcgc cctccttttg caacaagcag ctcgaatgtt ctagaaaaag
      781 gtggaaaata gtataaatac cgttgaaaat aaataccgaa caacatttgc tctaattgtg
      841 aaattagaaa tcttcaaact ataatcatgt cactttacca ctatttccgt ccagctcaac
      901 gttccgtttt tggtgatcct ctagagtcga cctgcaggca tgcaagcttg gcggccgcta
      961 cctgcaggat atctggatcc acgaagcttc ccatggtgac gtcaccggtt ctagatacct
     1021 aggtgagctc tggtaccctc tagtcaaggc cttaagtgag tcgtattacg tagcccgggc
     1081 ctagttctag acattctcta atgaaaaaat ctttcagttg aaattgaaaa tgagttaaag
     1141 ttggagtttt tattgaaaac agatttccgt gtgattagtg tttttagcga gtgtgacagg
     1201 acagcgaaaa aatatagaaa caagggggga actgaaaagc ttaggaatgc attgaacatg
     1261 agaaggggaa ggggaaggaa caaactagac aggaattatt ggaatttaat cacatttgga
     1321 gttttttttc tattcgacag aataattatc cagaacattt ttgtattaaa tatttatgca
     1381 tcatatgagt agtcggcttt gttgtgcatg acgagtttgt tatcgacgaa atagaagctg
     1441 tcagaacgag tctcgtttgg attgttgatc atgtcgtcca ctgaaaaaga gattagtctt
     1501 ttgaattgta ctttttagaa taatgactca ctgagttgtt gagagagttg agggaactca
     1561 tagatatgtt cacagttgtt tcgtgaattc ggaatacaga atccgaattc aaagtcaaaa
     1621 cacttcagaa ggcgatcttt gaagaagtga cgttcgatca ttcggaaatg atggattggg
     1681 atgtctccta ctttgaattc cacagttgct ccgaccgttt tgagtttcaa aaagtttgga
     1741 gcaaatctat aacgcacgta tcttgccgac tcctgtggcg actcatcatt ctcctgatcg
     1801 ttctccggtt tggcgatctc gaaaagcact tgctcagtgt ccagatcacg gatttggaac
     1861 ttggtgaact cgatgttata gatgttcgca gatggggagc ataagaatcc taaatttatg
     1921 ttttaaactg aaatccaaag ggagcaagat accttgagtg attcccggaa gtgctaaaac
     1981 gtcgttcgga gtgatttgag ctttcttcgc aagctccgat tccgttgtga ttccttgttc
     2041 ggtgcttggt ggtggccgtg gcatctggaa atatggaaaa gttcaacaaa aagaaaagag
     2101 aaaagaatga aatcggatat caagagttag ttgagcggtt tctctagttt tctgagtctc
     2161 acctgcgacg ggaaggtcgc cgagccgggt ggaatcgatc gttgttgctc ggctttcata
     2221 tcggtttggt tggaagcggc tgaaaacgga aagaagtgga agaaggaaaa gagtgtggtg
     2281 tgacaggaaa atggtaatta gagggtgcca aataaccagc tatattttgt ttttttttga
     2341 aaacattttt aaaaagaaaa atacgataat gatatcagat ggatttccgg aaaactggta
     2401 tgaaaaattt caaccttttt gagtacatgt aatcaaaata cactttgtaa attatcattt
     2461 ttattgaaac tccaccattt ttctatttat aacgctaata atttgaaaaa gaaacctatt
     2521 gcgaaccgcg gggtgaatcc caaaaacgaa tgcgttttgg tggagtgatt gattcgaatc
     2581 gaagaagaaa aagaagaaga cgtggaatag agagctcact cttaaccgag cagcacacac
     2641 cgacagaaaa aaaaatgaaa tgaatgaggg tcttcttctt cttcttcttc gaatgattga
     2701 cagaaatggg aaaaagagga agattgagaa gggaaaaagg aaggagaaaa gaagcagaag
     2761 aagacgtcag agaggagagg aacgagcgga aaagcagcgg gcgcaagtca tagaagtagc
     2821 agagctgggg agaagaagac actatccaag aaaggaatga cgagagagta tgcaaagggg
     2881 tatagggtgc agacagaata ggaacagaat aacagatgat gagccaagaa gagttgaaaa
     2941 gggcgatgaa tttgtcatgt aacttaattt gggtcaattt gagcatgatg aattgaaatc
     3001 atcccttgtt gggagttaat aaccggtttg ttatcagaaa ccctgtaata gaagggcgcc
     3061 ctaactttga gccaattcat cccggtttct gtcaaatata tcaaaaagtg gtcaactgac
     3121 aaattgtttt tgatattata ataaacattt tatccgttaa caattttcga atacttttta
     3181 caaggacttg gataaattgg ctcaaagacg aagtgagttt gctaccatca tggaatcagg
     3241 aggacatttc gacaatgtca tataatgttt gaaaaatgat tttaacaagg gtctcattcg
     3301 agataagcaa tgctctgagc aagtggctcg tcgtcttcaa gaatctcttg ttgatattca
     3361 tttggatcct gttttgcacg aagcatgtgc catggatatt cagagttttt gcaaaaaagt
     3421 tccgccaggt caaacaatct ttttaatttt tatatgatac ttcgggtgta tttttcttct
     3481 ttttcctgtt cattttcgct tttttctcac ttttttgtaa caaaatctct cacttttctg
     3541 taagtcttaa caaaagttca attggctcga tcaagtaaaa tttcggccgt atgaacgttt
     3601 tcaaaggttt tcattttttt ttaagttcat tcacaatatc atgaatctct cgtcctttgg
     3661 tttccggtaa gtagcgaaat aatataagca tacttataaa tgaaggaatt gaatatagaa
     3721 taaggaaagc ataactacca ataacggaat atagaggtaa tactgtaaat gtactgataa
     3781 ccaccattac agtattcatt gaatatgcca cagattgagc aatactacga tgtttttgag
     3841 gtaccagttc agaggatata aaccacgaaa ttggaccaac tccagttctg aaatttcatt
     3901 attcgattaa atcaaaatat atttgaaaaa cctcacccat aagtataacc gtagatgatc
     3961 aatgcaacta ggcatccgta tttcatcggg tcaatcagaa tacgtatttt tgcgaagaat
     4021 acgaatgcaa ttagagctaa attattgaga aatgtgaaga gaaggagaag cttgcgtcga
     4081 ccaactctgt aaacttattc atacaatttt gtcttcaaat tttgaacgtt taaaactatc
     4141 ttattcaatg agttagcggg tttttatagt gagttagttc acgattatct aataaaacta
     4201 tgtccaaaca tcctaactgc gaccgtattc tcactaactc gtcttaaaaa cgttgagatt
     4261 attttaaacc cttcttcagt acaactacag tactgatgca caaatacaat aataataata
     4321 cattcagaca tactctgaac aatgtaactt gtttttgtta tactgtggta acatttattg
     4381 aaatgaaata taaccttact tttcaatcac aattccacca gtaattgttc cagaaacata
     4441 agctaaaccc attgcagttg tactccattc agcaatttct gactcaacat ttgcattttc
     4501 tagaaagaaa gtagaggata aaagaagagc ccataatccg acagggccct gtgaaattgt
     4561 tatccgctgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     4621 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaaga ccaggaaccg taaaaaggcc
     4681 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     4741 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     4801 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     4861 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     4921 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     4981 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     5041 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     5101 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     5161 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     5221 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     5281 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     5341 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     5401 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     5461 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     5521 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctga ccccagtgct
     5581 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     5641 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     5701 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     5761 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     5821 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     5881 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     5941 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     6001 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     6061 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     6121 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     6181 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     6241 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     6301 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     6361 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     6421 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc
     6481 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa
     6541 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
     6601 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac
     6661 tatgcggcat cagagcagat tgcactgaga gtgcaccata tgcggtgtga aataccgcac
     6721 agatgcgtaa ggagaaaata ccgcatcagg cgccattcgc cattcaggct gcgcaactgt
     6781 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6841 gctgcaaggc gattaagttg ggtaacgcca gggttttccc cagtcacgac gtcacagcac
     6901 gtgtgttttt gaacacggcg atatgtatct gtagatcatc cccaattttc aacaatgaat
     6961 ctaaatactg aactttttgt gtaatcttta gtgcagaaaa atgaccaaaa tcagcgattt
     7021 tccgattata cccaaaatat ctcagctcaa tgagcaccta taaaaatgta aaaataaaga
     7081 aagtagtcga accatatttt gtaaaggcta aaaaactgaa taaattttta tccatccagc
     7141 gcggcgttac aatccgtggc ccatgtcacg cacggctttc aaagaaaaaa aaacagtctg
     7201 tttgcgcgac atcgatctgg caaaaatcct aaaaacgact atgcgtggct cactttatat
     7261 taatatgaaa aatacaaact tggtggtgca ggtgctgcag aggccatcga tgaagaagtg
     7321 gaggccttgt tgtcgatcta caaaattgta tggttgtttg agattttcct tgaaacgctc
     7381 tcaatcttgg atatgatata aattctggat aagatatgat ggatatgatc tatgtttgat
     7441 agagtgcatt tgaaaggata acggccctgc agacattttt gaaaaaataa caaacattta
     7501 attttctcac tcgtttaggc tattccccta tttttgatat tccttcgcac atatgaaaac
     7561 tacttttttt cgaaactgtt aactccagaa tttataaatc tatagccctt acttgattat
     7621 ttattatcat ggttactcac ctcatgtctc tttctctttt caagaagtct tcgttcatat
     7681 ctgttgtctc tttcctcctt ttgaatctct gctttgctct tcgccattgt ttcctgaaaa
     7741 taatgtaact tgaattgtgt aatatacttt tttaatttga atttggcttg taacgcggaa
     7801 tcactacgtg cgggatcatt tcttactaga aaacccagaa aatgccatat ttcacttatc
     7861 tcggggtcat ttctaattag aaaagctaca aaaaccattt ctaattacgg tgactttaga
     7921 actggtcgaa atagctaatt agaaatgggt ttttgctaat tagaggtgac tctatgttat
     7981 ctgagtaagc ctcgtttgta tgtaaactca cagtactaat aaatgcaaga cacccgggtt
     8041 tgtctagata tgaaataatt gaaatatcaa ttctgacaga caataatggt aatcttgata
     8101 aggagttcca cgcccaggag aacacgttag ttttcttgtt tttgattgcg tgcgttattt
     8161 tggagaaaaa ctcgattttt tacaaaataa ttttttgaaa ggaacactgt tcaataagtt
     8221 ttgtcttttt tctcagttgt gatacggttt tttattcttt tttgtagtta tacagaagac
     8281 cgttacgaaa cagactgata tcgaaacaaa cactgatctt acttgcactt ataatacgac
     8341 tcactagtag gaacagttat gtttggtata ttgggaatgt attctgtcat ttaaggcaaa
     8401 aaccccatag acactactcc actttcaaat tatcaacttc tactcacctt ccactgagcc
     8461 tcaaacccaa accttcttcc gatctttctt gcatcgtgct catcaatact tgtgatttta
     8521 gcaagaaaaa cgccgtcctc gaaatcttga caaacaatag gggaaacccc aaaaagcaaa
     8581 agcaggaaaa ttaatttcat aaactttcac ttgattgaaa aattccaaaa atactccaaa
     8641 actttttcac gatgagcatg atttgacgtc atgagaggcc cagacgtgcg aagaaataaa
     8701 aattgcggtc ataaactgaa acgtaacata tgataaggta ttttgtgtgc ggttttttct
     8761 atgatgtttc aaaaaaattt atcagaagta aaaaacataa gaagtgtaca cagaacattg
     8821 tgtagatttt gggatagaat tattattaat ggggggaggg agcacaattt ttttcgactt
     8881 tttattacga attaattcta tttttgaatc tacacaattt cattgttaga ggtgacttaa
     8941 aagaagctaa aaaacaaaga aattaagaga agttttttga taataacaaa aatagggggt
     9001 gggagcacag ggagaaagag catgtaggga tgttgaagag taattggact tagaagtcag
     9061 aggcacgggc gcgagatggc gatctgatga cagcggccga tgcggagctt cgcgatgcgc
     9121 tagctccacg cgttgcagat ccactagttt acttgctgga agtgtacttg gtgacggcct
     9181 tggttccctc agacacggcg tgcttggcaa gttctcctgg gagaatcaaa cggacagcgg
     9241 tttgaatttc gcgggatgag atcgttgagc gtttgttgta atgagcaaga cgggaagctt
     9301 ccgaagcgat gcgttcgaat acatcgttga cgaaggagtt catgatagac atggccttgg
     9361 aggagactcc ggtgtctggg tgaacttgct tgagaacacg gtagatgtag acggagtacg
     9421 attccttgcg ggcatgacgt ctcttctttc cgtcctttgg cttggcaacg acggtcttgg
     9481 cggccttctt ggctcccttg gcagatggct ttggtggact agttctagag cggccgccac
     9541 cggtggagct ccaccgatcc cccgggcatt tgtatagttc atccatgcca tgtgtaatcc
     9601 cagcagctgt tacaaactca agaaggacca tgtggtctct cttttcgttg ggatctttcg
     9661 aaagggcaga ttgtgtggac aggtaatggt tgtctggtaa aaggacaggg ccatcgccaa
     9721 ttggagtatt ttgttgataa tggtctgcta gttgaacgct tccatcttca atgttgtgtc
     9781 taattttgaa gttctgaaaa tttaaatcag attagttagt tagtaaaatc atgtttaaac
     9841 ttacaacttt gattccattc ttttgtttgt ctgccatgat gtatacattg tgtgagttat
     9901 agttgtattc caatttgtgt ccaagaatgt ttccatcttc tttaaaatca atacctttta
     9961 actcgattct attaacaagg gtatcacctt caaacttgac ttcagcacct gaaaatttaa
    10021 atatgtatgg ttagttagta ccgaactgtt taaacttacg tgtcttgtag ttcccgtcat
    10081 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
    10141 catgccgttt catatgatct gggtatctcg agaagcattg aacaccataa cagaaagtag
    10201 tgacaagtgt tggctgaaaa tttaaataat cagggttagt tagtatatat atgtttaaac
    10261 ttacccatgg aacaggtagt tttccagtag tgcaaataaa tttaagggta agttttccgt
    10321 atgttgcatc accttcaccc tctccactga cagaaaattt gtgcccatta acatcaccat
    10381 ctaattcaac aagaattggg acaactccag tgaaaagttc ttctccttta ctcatttttt
    10441 ctaccggtac cgaagttcct atactttcta gagaatagga acttcacgcg cccaagcgag
    10501 gacaattctc atcgttcgcc taatttcttt gcttattttt tactagtttt ccttcctcct
    10561 ctatatattt ttatgttttc ttttaaacct tcctccatat ttctgaattg aatgttgttg
    10621 catattgtgt gtccttattt catttccatc atccttttgt gtgtttcccc cttccaccaa
    10681 ttccccccac gtcctaatga aatgaaagcg agaagataaa taaacttgca cattgtgttc
    10741 agatgagagg agcggaatac gaattgggag acggaaagag aaaaaaaaat gtttagattt
    10801 ggattgaatt aattttttag aaagcaagaa aaaaaacaca aagagaataa ataaataata
    10861 ggaaaaaaaa caaatattca gtatttaaaa tttgaaaatt caacgacgtt ggcgtcgatc
    10921 atccggatta cttgtatggc ccctgatctc tgcaggttac ttgctggaag tgtacttggt
    10981 gacggccttg gttccctcag acacggcgtg cttggcaagt tctcctggga gaatcaaacg
    11041 gacagcggtt tgaatttcgc gggatgagat cgttgagcgt ttgttgtaat gagcaagacg
    11101 ggaagcttcc gaagcgatgc gttcgaatac atcgttgacg aaggagttca tgatagacat
    11161 ggccttggag gagactccgg tgtctgggtg aacttgcttg agaacacggt agatgtagac
    11221 ggagtacgat tccttgcggg catgacgtct cttctttccg tcctttggct tggcaacgac
    11281 ggtcttggcg gccttcttgg ctcccttggc agatggcttt ggtggactag ttctagagcg
    11341 gccgccaccg gtcttataca attcatccat gccacctgtc gagtgccgcc cttcagctct
    11401 ttcatattgc tcaacgatag tgtaatcctc gttgtgggag gtgatatcca gtttaatatt
    11461 aacattgtac gctcctggca gctgaactgg ttttttggct ttgtaagttg tcttcacctg
    11521 aaaatttaaa tcagattagt tagttagtaa aatcatgttt aaacttacct cagcatcgta
    11581 atgtcctcca tctttcaatt taagacgttg tttaatctct cccttaagag caccgtcttc
    11641 agggtacatt ctttcacttg aagcttccca tcccatagtc ttcttctgca taacaggtcc
    11701 atccgagggg aaattcgttc ctcgaagttt gactttataa ataaattctc catcttggag
    11761 gctgaaaatt taaatatgta tggttagtta gtaccgaact gtttaaactt accttgagtc
    11821 ttgcgtcact gtaacaa
//
