LOCUS       sequence-170556-        7333 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cloning plasmid for MosSCI transgenesis in C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Morales-Martinez A, Dobrzynska A, Askjaer P
  TITLE     Inner nuclear membrane protein LEM-2 is required for correct nuclear
            separation and morphology in C. elegans.
  JOURNAL   J Cell Sci. 2015 Mar 15;128(6):1090-6. doi: 10.1242/jcs.164202. Epub
            2015 Feb 4.
  PUBMED    25653391
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/jcs.164202"; journalName: "J Cell Sci"; date: "2015-03-15- 
            15"; volume: "128"; issue: "6"; pages: "1090-6"
COMMENT     Sequence Label: pBN9
FEATURES             Location/Qualifiers
     source          1..7333
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    35..55
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    1516..1540
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1689..1700)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(3873..3897)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4944..4964)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(4972..4989)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4972..4988)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4981..5003)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     5316..5338
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5376..5394)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5462..5566
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5567..5635,5636..6427)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5785..5804)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6598..7186
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7087..7106
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     join(7313..7333,1..2)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(7332..7333,1..15)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(7332..7333,1..15)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ggaaacagct atgaccatga ttacgccaag ctatcaactt tgtatagaaa agttggaggg
       61 acttccagct gtctgcgagc cagtccaaaa gggagagcca ggatccaccg gagagccagg
      121 accagtcgga ccaccaggac catccggaca accaggaaac gacggaaccc caggtaatca
      181 tttcatagtt tttcaatcca tatctaatca ccacatcttt caggacaacc tggaccaaag
      241 ggaccaccag gaccagacgg caagccagga gccgacggaa acccaggaca gccaggacca
      301 gtcggaccac caggaacccc aggagagcgt ggaatctgtc caaagtactg cgccatcgat
      361 ggaggaatct tcttcgagga cggaactcgc cgctaagcgg atcgccttta atttattctt
      421 tgccttatgc gttgctcaaa ataaaacatt atttcatcca aaataatttt ttgaggtttt
      481 agatgaattt gatagtttag cttgtcactt tctcctgtgc gaagaaactt gctaagcagg
      541 taagcagcat ccttatcaag tttcgacctt atctctcagt agtcgcatac gtcgtatccc
      601 tcatctgctt tgttttagcc acttcaaggg aattcagatc actttttctc atgtgttttc
      661 aaatttactt ttgtggagtg accttaattg accctctcgt cataagtgga cggtgggtag
      721 ttacttaaca aatttcgagt acaggatagt gtcaatggca tgagacatgt agtagccaag
      781 caattatggc gaataagttt caaaaaccaa cattctgtca aatacaaaga cctgcaacat
      841 tagagaattc attaaataca tcttcgagga aatcaaattt tcccgtagac gatcccgtag
      901 acctacttgt acaatatgtt ctagatatac actccgaact taaaatatct cgaacatttg
      961 tgttgaatat ttattttaat tctaggtttt tcaaattccg cacgattttt gccatactac
     1021 tgcccaatag tttacattta ttgggcctgg atctgtaagg cacttttggg agctcccttt
     1081 cttttccttc aaattaccca tacagatcta taaatcacaa tttaatacaa gattttattc
     1141 aagttaaaaa cataaaaggg gctttgcggt ggtccccatt tcaccagaga actataaaaa
     1201 atataaattt aacatcaaat gttgcaattg attccctctc aaagctgctt gaagaaccct
     1261 gattctgtca agcctatgaa gatttaaaaa aaattgggaa gacccttagt tccaaacaag
     1321 tgtcgttgac ccagtaggcg atattctgaa aagtcataaa atggggttgc taaaaaattg
     1381 gtcgctaact tacatttagc taggaatgtt aattggaata actcataatt tacagtaaaa
     1441 ttatcaaaaa atagcaaaaa tcaatagagg accgctacac aggcatccaa cagtacgagt
     1501 tctgcgtttt tgaattcaag tttgtacaaa aaagcaggct ctttgagcca atttatccaa
     1561 gtccttgtaa aaagtattcg aaaattgtta acggataaaa tgtttattat aatatcaaaa
     1621 acaatttgtc agttgaccac tttttgatat atttgacaga aaccgggatg aattggctca
     1681 aagttagggc gcccttctat tacagggttt ctgataacaa accggttatt aactcccaac
     1741 aagggatgat ttcaattcat catgctcaaa ttgacccaaa ttaagttaca tgacaaattc
     1801 atcgcccttt tcaactcttc ttggctcatc atctgttatt ctgttcctat tctgtctgca
     1861 ccctataccc ctttgcatac tctctcgtca ttcctttctt ggatagtgtc ttcttctccc
     1921 cagctctgct acttctatga cttgcgcccg ctgcttttcc gctcgttcct ctcctctctg
     1981 acgtcttctt ctgcttcttt tctccttcct ttttcccttc tcaatcttcc tctttttccc
     2041 atttctgtca atcattcgaa gaagaagaag aagaagaccc tcattcattt catttttttt
     2101 tctgtcggtg tgtgctgctc ggttaagagt gagctctcta ttccacgtct tcttcttttt
     2161 cttcttcgat tcgaatcaat cactccacca aaacgcattc gtttttggga ttcaccccgc
     2221 ggttcgcaat aggtttcttt ttcaaattat tagcgttata aatagaaaaa tggtggagtt
     2281 tcaataaaaa tgataattta caaagtgtat tttgattaca tgtactcaaa aaggttgaaa
     2341 tttttcatac cagttttccg gaaatccatc tgatatcatt atcgtatttt tctttttaaa
     2401 aatgttttca aaaaaaaaca aaatatagct ggttatttgg caccctctaa ttaccatttt
     2461 cctgtcacac cacactcttt tccttcttcc acttctttcc gttttcagcc gcttccaacc
     2521 aaaccgatat gaaagccgag caacaacgat cgattccacc cggctcggcg accttcccgt
     2581 cgcaggtgag actcagaaaa ctagagaaac cgctcaacta actcttgata tccgatttca
     2641 ttcttttctc ttttcttttt gttgaacttt tccatatttc cagatgccac ggccaccacc
     2701 aagcaccgaa caaggaatca caacggaatc ggagcttgcg aagaaagctc aaatcactcc
     2761 gaacgacgtt ttagcacttc cgggaatcac tcaaggtatc ttgctccctt tggatttcag
     2821 tttaaaacat aaatttagga ttcttatgct ccccatctgc gaacatctat aacatcgagt
     2881 tcaccaagtt ccaaatccgt gatctggaca ctgagcaagt gcttttcgag atcgccaaac
     2941 cggagaacga tcaggagaat gatgagtcgc cacaggagtc ggcaagatac gtgcgttata
     3001 gatttgctcc aaactttttg aaactcaaaa cggtcggagc aactgtggaa ttcaaagtag
     3061 gagacatccc aatccatcat ttccgaatga tcgaacgtca cttcttcaaa gatcgccttc
     3121 tgaagtgttt tgactttgaa ttcggattct gtattccgaa ttcacgaaac aactgtgaac
     3181 atatctatga gttccctcaa ctctctcaac aactcagtga gtcattattc taaaaagtac
     3241 aattcaaaag actaatctct ttttcagtgg acgacatgat caacaatcca aacgagactc
     3301 gttctgacag cttctatttc gtcgataaca aactcgtcat gcacaacaaa gccgactact
     3361 catatgatgc ataaatattt aatacaaaaa tgttctggat aattattctg tcgaatagaa
     3421 aaaaaactcc aaatgtgatt aaattccaat aattcctgtc tagtttgttc cttccccttc
     3481 cccttctcat gttcaatgca ttcctaagct tttcagttcc ccccttgttt ctatattttt
     3541 tcgctgtcct gtcacactcg ctaaaaacac taatcacacg gaaatctgtt ttcaataaaa
     3601 actccaactt taactcattt tcaatttcaa ctgaaagatt ttttcattag agaatgtcta
     3661 gaactaggcc cgggctacgt aatacgactc acttaaggcc ttgactagag ggtaccagag
     3721 ctcacctagg tatctagaac cggtgacgtc accatgggaa gcttcgtgga tccagatatc
     3781 ctgcaggtag cggccgcatg ctacagccgg ctcctcgaga cgtacggtgc gcgcgatgca
     3841 ttcgaagatc tgcccactag tgagtcgtat taacccagct ttcttgtaca aagtggatag
     3901 aatcaagcat gctccgcgtt tgctgtacta tttctcgcag agcagttttt tttctcacac
     3961 tttattattc tgtactgccc ctccacgcta taattttttg catagagggc cataaaggaa
     4021 aacggtacgg taaaaataat gggtcctttt taccgttgtg tagattgttt ctaacgggat
     4081 gcaaattttc actaaaactc tggaaaactg atggaattct acgggcagcc ggcaatttta
     4141 gatatttgaa gaatccttct tgatcagtgc caagctttcc catggaccaa agatgtggat
     4201 ccaagatcaa atgcacagga tctgaactcc tttgttccaa aaacaaggat ttcttttcta
     4261 tagtttgacc taaatattat agtagaacat atacatttga tgatttcatg aataatttat
     4321 tgttaaaaaa taatacatgt gttaaaaaat aattctaaaa aattaattgg agcgtccttg
     4381 gcggatatgg cggattggaa ggtcataagt aactcgaact aacctgtaaa tataattaat
     4441 ttagaagtaa tcattttgaa atgtttctaa atttttgtaa acgtaatcaa tgatttactt
     4501 acaaaatttt tcgaaatatt agtagaaata taaaaaacca gtaaaattca aaagaggcca
     4561 gccacacatg catcttacaa tacgaatgat gcgttttttg tcgcctcgga gaaggaatca
     4621 agcaggctcc gcgttttctg tactatttct cccagagccg tttattttgc atagggggtg
     4681 ggtcataaac ggagacgttt tggtataaaa tttgcccctt cttccaccgt tgaaaaaatt
     4741 ggaaaatgaa ccgggacagg aaaggggatt tcaggataat ggttcaatta tcgatgctac
     4801 tgtcatatct gagtagtgga tttgagcaag gtttccaaat tttgagtaca agcgtctagt
     4861 gcatgtgtca aaaaagtcgg gcaaaaatta gttttaatca ataaggttta aagatcagaa
     4921 atttttgtct gacctaatgg gcccaacttt attatacata gttgataatt cactggccgt
     4981 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     5041 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     5101 acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc ttacgcatct
     5161 gtgcggtatt tcacaccgca tatggtgcac tctcagtgca atctgctctg atgccgcata
     5221 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     5281 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     5341 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     5401 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     5461 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     5521 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     5581 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     5641 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     5701 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     5761 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     5821 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     5881 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     5941 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6001 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6061 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6121 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6181 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6241 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6301 agcactgggg tcagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6361 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     6421 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     6481 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     6541 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     6601 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     6661 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     6721 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     6781 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     6841 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     6901 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     6961 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7021 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7081 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7141 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7201 ggccttttta cggttcctgg tcttttgctg gccttttgct cacatgttct ttcctgcgtt
     7261 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccagcggata
     7321 acaatttcac aca
//
