LOCUS       sequence-170558-        9641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Heat shock-induced FLP expression plasmid for C. elegans.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Munoz-Jimenez C, Ayuso C, Dobrzynska A, Torres-Mendez A, Ruiz PC, 
            Askjaer P
  TITLE     An Efficient FLP-Based Toolkit for Spatiotemporal Control of Gene 
            Expression in Caenorhabditis elegans.
  JOURNAL   Genetics. 2017 Aug;206(4):1763-1778. doi: 
            10.1534/genetics.117.201012. Epub 2017 Jun 23.
  PUBMED    28646043
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1534/genetics.117.201012"; journalName: "Genetics"; date: 
            "2017-08"; volume: "206"; issue: "4"; pages: "1763-1778"
COMMENT     Sequence Label: pBN262
FEATURES             Location/Qualifiers
     source          1..9641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             385..396
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(545..569)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2030..2050)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(2070..2086)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2070..2086)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2083..2105)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(2232..2820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2312..2331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2991..3782,3783..3851))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3614..3633
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3852..3956)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     4024..4042
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(4080..4102)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     4415..4437
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4429..4446
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4430..4446
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4454..4474
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    5521..5545
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(6106..6122)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6107..6123)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 atgagggtct tcttcttctt cttcttcgaa tgattgacag aaatgggaaa aagaggaaga
       61 ttgagaaggg aaaaaggaag gagaaaagaa gcagaagaag acgtcagaga ggagaggaac
      121 gagcggaaaa gcagcgggcg caagtcatag aagtagcaga gctggggaga agaagacact
      181 atccaagaaa ggaatgacga gagagtatgc aaaggggtat agggtgcaga cagaatagga
      241 acagaataac agatgatgag ccaagaagag ttgaaaaggg cgatgaattt gtcatgtaac
      301 ttaatttggg tcaatttgag catgatgaat tgaaatcatc ccttgttggg agttaataac
      361 cggtttgtta tcagaaaccc tgtaatagaa gggcgcccta actttgagcc aattcatccc
      421 ggtttctgtc aaatatatca aaaagtggtc aactgacaaa ttgtttttga tattataata
      481 aacattttat ccgttaacaa ttttcgaata ctttttacaa ggacttggat aaattggctc
      541 aaagagcctg cttttttgta caaacttgaa ttcaaaaacg cagaactcgt actgttggat
      601 gcctgtgtag cggtcctcta ttgatttttg ctattttttg ataattttac tgtaaattat
      661 gagttattcc aattaacatt cctagctaaa tgtaagttag cgaccaattt tttagcaacc
      721 ccattttatg acttttcaga atatcgccta ctgggtcaac gacacttgtt tggaactaag
      781 ggtcttccca atttttttta aatcttcata ggcttgacag aatcagggtt cttcaagcag
      841 ctttgagagg gaatcaattg caacatttga tgttaaattt atatttttta tagttctctg
      901 gtgaaatggg gaccaccgca aagccccttt tatgttttta acttgaataa aatcttgtat
      961 taaattgtga tttatagatc tgtatgggta atttgaagga aaagaaaggg agctcccaaa
     1021 agtgccttac agatccaggc ccaataaatg taaactattg ggcagtagta tggcaaaaat
     1081 cgtgcggaat ttgaaaaacc tagaattaaa ataaatattc aacacaaatg ttcgagatat
     1141 tttaagttcg gagtgtatat ctagaacata ttgtacaagt aggtctacgg gatcgtctac
     1201 gggaaaattt gatttcctcg aagatgtatt taatgaattc tctaatgttg caggtctttg
     1261 tatttgacag aatgttggtt tttgaaactt attcgccata attgcttggc tactacatgt
     1321 ctcatgccat tgacactatc ctgtactcga aatttgttaa gtaactaccc accgtccact
     1381 tatgacgaga gggtcaatta aggtcactcc acaaaagtaa atttgaaaac acatgagaaa
     1441 aagtgatctg aattcccttg aagtggctaa aacaaagcag atgagggata cgacgtatgc
     1501 gactactgag agataaggtc gaaacttgat aaggatgctg cttacctgct tagcaagttt
     1561 cttcgcacag gagaaagtga caagctaaac tatcaaattc atctaaaacc tcaaaaaatt
     1621 attttggatg aaataatgtt ttattttgag caacgcataa ggcaaagaat aaattaaagg
     1681 cgatccgctt agcggcgagt tccgtcctcg aagaagattc ctccatcgat ggcgcagtac
     1741 tttggacaga ttccacgctc tcctggggtt cctggtggtc cgactggtcc tggctgtcct
     1801 gggtttccgt cggctcctgg cttgccgtct ggtcctggtg gtccctttgg tccaggttgt
     1861 cctgaaagat gtggtgatta gatatggatt gaaaaactat gaaatgatta cctggggttc
     1921 cgtcgtttcc tggttgtccg gatggtcctg gtggtccgac tggtcctggc tctccggtgg
     1981 atcctggctc tcccttttgg actggctcgc agacagctgg aagtccctcc aacttttcta
     2041 tacaaagttg atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     2101 ccgctggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     2161 gcaggaaaga acatgtgagc aaaaggccag caaaagacca ggaaccgtaa aaaggccgcg
     2221 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     2281 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     2341 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     2401 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     2461 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     2521 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     2581 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     2641 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     2701 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     2761 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     2821 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     2881 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     2941 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     3001 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     3061 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctgaccc cagtgctgca
     3121 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     3181 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     3241 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     3301 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     3361 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     3421 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     3481 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     3541 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     3601 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     3661 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     3721 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     3781 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     3841 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     3901 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     3961 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     4021 aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt tcggtgatga cggtgaaaac
     4081 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     4141 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggctg gcttaactat
     4201 gcggcatcag agcagattgc actgagagtg caccatatgc ggtgtgaaat accgcacaga
     4261 tgcgtaagga gaaaataccg catcaggcgc cattcgccat tcaggctgcg caactgttgg
     4321 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     4381 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     4441 gccagtgaat tatcaactat gtataataaa gttgggccca ttaggtcaga caaaaatttc
     4501 tgatctttaa accttattga ttaaaactaa tttttgcccg acttttttga cacatgcact
     4561 agacgcttgt actcaaaatt tggaaacctt gctcaaatcc actactcaga tatgacagta
     4621 gcatcgataa ttgaaccatt atcctgaaat cccctttcct gtcccggttc attttccaat
     4681 tttttcaacg gtggaagaag gggcaaattt tataccaaaa cgtctccgtt tatgacccac
     4741 cccctatgca aaataaacgg ctctgggaga aatagtacag aaaacgcgga gcctgcttga
     4801 ttccttctcc gaggcgacaa aaaacgcatc attcgtattg taagatgcat gtgtggctgg
     4861 cctcttttga attttactgg ttttttatat ttctactaat atttcgaaaa attttgtaag
     4921 taaatcattg attacgttta caaaaattta gaaacatttc aaaatgatta cttctaaatt
     4981 aattatattt acaggttagt tcgagttact tatgaccttc caatccgcca tatccgccaa
     5041 ggacgctcca attaattttt tagaattatt ttttaacaca tgtattattt tttaacaata
     5101 aattattcat gaaatcatca aatgtatatg ttctactata atatttaggt caaactatag
     5161 aaaagaaatc cttgtttttg gaacaaagga gttcagatcc tgtgcatttg atcttggatc
     5221 cacatctttg gtccatggga aagcttggca ctgatcaaga aggattcttc aaatatctaa
     5281 aattgccggc tgcccgtaga attccatcag ttttccagag ttttagtgaa aatttgcatc
     5341 ccgttagaaa caatctacac aacggtaaaa aggacccatt atttttaccg taccgttttc
     5401 ctttatggcc ctctatgcaa aaaattatag cgtggagggg cagtacagaa taataaagtg
     5461 tgagaaaaaa aactgctctg cgagaaatag tacagcaaac gcggagcatg cttgattcta
     5521 tccactttgt acaagaaagc tgggttaata cgactcacta gtgggcagat cttcgaatgc
     5581 atcgcgcgca ccgtacgtct cgaggagccg gctgtagcat gcggccgcca agcttgcatg
     5641 cctgcaggtc gactctagag gatcaccaaa aacggaacgt tgagctggac ggaaatagtg
     5701 gtaaagtgac atgattatag tttgaagatt tctaatttca caattagagc aaatgttgtt
     5761 cggtatttat tttcaacggt atttatacta ttttccacct ttttctagaa cattcgagct
     5821 gcttgttgca aaaggagggc gactcacatt cggtacatgg aaaagtagtg tacacaataa
     5881 agagacccag atacattttc cgtctgcgtc tctttgcacc caccgggagt attttcaaac
     5941 gaatgcatct aggaccttct agaacattct gtaaggctgc agaatgcggg tatataagga
     6001 aagcgggctc agaggaagcc aacacgcttt gttctagtgc atctaaaaaa cttcgaaaat
     6061 cctcatcgga tcccccgggc tgcaggaatt cgatatcaag cttatcgata ccgtcgacct
     6121 cgagggggcg gtaccaaaaa tgccacaatt cgacattctc tgtaagacac caccaaaagt
     6181 cctcgtccgt caattcgtcg agcgtttcga gcgtccatcc ggagagaaga tcgccctctg
     6241 cgccgccgag ctcacctacc tctgctggat gatcacccac aacggaaccg ccatcaagcg
     6301 tgccaccttc atgtcctaca acaccatcat ctccaactcc ctctccttcg acatcgtcaa
     6361 caagtccctc caattcaagt acaagaccca aaaggccacc atcctcgagg cctccctcaa
     6421 ggtaagttta aacatatata tactaactaa ccctgattat ttaaattttc agaagctcat
     6481 cccagcctgg gagttcacca tcatcccata ctacggacaa aagcaccaat ccgacatcac
     6541 cgacatcgtc tcctccctcc aactccaatt cgagtcctcc gaggaggccg acaagggaaa
     6601 ctcccactcc aagaagatgc tcaaggccct cctctccgag ggagagtcca tctgggagat
     6661 caccgagaag atcctcaact ccttcgagta cacctcccgt ttcaccaaga ccaagaccct
     6721 ctaccaattc ctcttcctcg ccaccttcat caactgcgga cgtttctccg acatcaagaa
     6781 cgtcgaccca aagtccttca agctcgtcca aaacaagtac ctcggagtca tcatccaatg
     6841 cctcgtcacc gagaccaaga cctccgtctc ccgtcacatc tacttcttct ccgcccgtgg
     6901 acgtatcgac ccactcgtct acctcgacga gttcctccgt aactccgagc cagtcctcaa
     6961 gcgtgtcaac cgtaccggaa actcctcctc caacaagcaa gagtaccaac tcctcaagga
     7021 caacctcgtc cgttcctaca acaaggccct caagaagaac gccccatact ccatcttcgc
     7081 catcaagaac ggaccaaagt cccacatcgg acgtcacctc atgacctcct tcctctccat
     7141 gaaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt ttcagggact
     7201 caccgagctc accaacgtcg tcggaaactg gtccgacaag cgtgcctccg ccgtcgcccg
     7261 taccacctac acccaccaaa tcaccgccat cccagaccac tacttcgccc tcgtctcccg
     7321 ttactacgcc tacgacccaa tctccaagga gatgatcgcc ctcaaggacg agaccaaccc
     7381 aatcgaggag tggcaacaca tcgagcaact caagggatcc gccgagggat ccatccgtta
     7441 cccagcctgg aacggaatca tctcccaaga ggtcctcgac tacctctcct cctacatcaa
     7501 ccgtcgtatc taacgcgtat gtatctgcat atccttcaaa tgttgtccat attgtatcag
     7561 taaattataa tgcccccttt ttatatattc tccttatcat gtaaatgttc tattgatttt
     7621 ttgctggtgt aaaacgtttt tatagtaaat cctctccaca gtgaatttgt taaattgtat
     7681 caaaacaaga cattgattaa atttgttcga gacttgtgga ttgtaaaata ttttgcagaa
     7741 aatggcaata ttcatttaga aacattactt ccataatagg aaaaactatt tatgagtttt
     7801 catgtgccta tgcaagttcc cactctgtgt gaatttcatc atacaaattg agcaaacata
     7861 aggtttatgt ccagtatggg ttctcagatg ttttgtaaga gttgagctgt cggtgaatgc
     7921 cttctcacat tgagcacatg gatacggtct ctctccagtg tgtgttctga aataatgaaa
     7981 aatagtcttg caatatgttt ctactattcg agaaaaaatg taattttttt tcttatggta
     8041 acccggcttt ccccgggcct agttctagac attctctaat gaaaaaatct ttcagttgaa
     8101 attgaaaatg agttaaagtt ggagttttta ttgaaaacag atttccgtgt gattagtgtt
     8161 tttagcgagt gtgacaggac agcgaaaaaa tatagaaaca aggggggaac tgaaaagctt
     8221 aggaatgcat tgaacatgag aaggggaagg ggaaggaaca aactagacag gaattattgg
     8281 aatttaatca catttggagt tttttttcta ttcgacagaa taattatcca gaacattttt
     8341 gtattaaata tttatgcatc atatgagtag tcggctttgt tgtgcatgac gagtttgtta
     8401 tcgacgaaat agaagctgtc agaacgagtc tcgtttggat tgttgatcat gtcgtccact
     8461 gaaaaagaga ttagtctttt gaattgtact ttttagaata atgactcact gagttgttga
     8521 gagagttgag ggaactcata gatatgttca cagttgtttc gtgaattcgg aatacagaat
     8581 ccgaattcaa agtcaaaaca cttcagaagg cgatctttga agaagtgacg ttcgatcatt
     8641 cggaaatgat ggattgggat gtctcctact ttgaattcca cagttgctcc gaccgttttg
     8701 agtttcaaaa agtttggagc aaatctataa cgcacgtatc ttgccgactc ctgtggcgac
     8761 tcatcattct cctgatcgtt ctccggtttg gcgatctcga aaagcacttg ctcagtgtcc
     8821 agatcacgga tttggaactt ggtgaactcg atgttataga tgttcgcaga tggggagcat
     8881 aagaatccta aatttatgtt ttaaactgaa atccaaaggg agcaagatac cttgagtgat
     8941 tcccggaagt gctaaaacgt cgttcggagt gatttgagct ttcttcgcaa gctccgattc
     9001 cgttgtgatt ccttgttcgg tgcttggtgg tggccgtggc atctggaaat atggaaaagt
     9061 tcaacaaaaa gaaaagagaa aagaatgaaa tcggatatca agagttagtt gagcggtttc
     9121 tctagttttc tgagtctcac ctgcgacggg aaggtcgccg agccgggtgg aatcgatcgt
     9181 tgttgctcgg ctttcatatc ggtttggttg gaagcggctg aaaacggaaa gaagtggaag
     9241 aaggaaaaga gtgtggtgtg acaggaaaat ggtaattaga gggtgccaaa taaccagcta
     9301 tattttgttt ttttttgaaa acatttttaa aaagaaaaat acgataatga tatcagatgg
     9361 atttccggaa aactggtatg aaaaatttca acctttttga gtacatgtaa tcaaaataca
     9421 ctttgtaaat tatcattttt attgaaactc caccattttt ctatttataa cgctaataat
     9481 ttgaaaaaga aacctattgc gaaccgcggg gtgaatccca aaaacgaatg cgttttggtg
     9541 gagtgattga ttcgaatcga agaagaaaaa gaagaagacg tggaatagag agctcactct
     9601 taaccgagca gcacacaccg acagaaaaaa aaatgaaatg a
//
