LOCUS       sequence-170560-        9863 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  C-terminal flag-tagged protein expression in mammalian cells, 
            lentiviral vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9863)
  AUTHORS   Yun YS, Kim KH, Tschida B, Sachs Z, Noble-Orcutt KE, Moriarity BS, 
            Ai T, Ding R, Williams J, Chen L, Largaespada D, Kim DH
  TITLE     mTORC1 Coordinates Protein Synthesis and Immunoproteasome Formation 
            via PRAS40 to Prevent Accumulation of Protein Stress.
  JOURNAL   Mol Cell. 2016 Feb 18;61(4):625-39. doi: 
            10.1016/j.molcel.2016.01.013. Epub 2016 Feb 11.
  PUBMED    26876939
REFERENCE   2  (bases 1 to 9863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molcel.2016.01.013"; journalName: "Mol Cell"; date: 
            "2016-02-18- 18"; volume: "61"; issue: "4"; pages: "625-39"
COMMENT     Sequence Label: pcsII-pb6-flag
FEATURES             Location/Qualifiers
     source          1..9863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        382..580
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             598..778
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    825..950
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1442..1675
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1860..1904
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2178..2295
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2351..3513
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2582..3504
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3461..3481
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3532..3541
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4251..4274
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    4324..4912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4377..4397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4795..4806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5431..5611
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5637..5654)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5643..5867
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5913..6341
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6000..6019)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6210..6231
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6350..6370)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6355..6684
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6535..6670
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6597..6616
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6732..6779
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6798..7172
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7302..7423
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7339..7358)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7393..7412
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7472..7488)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7472..7488)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7485..7507)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7496..7512
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7520..7526,7527..7544,7545..7550))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7565..7586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7703..7720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7874..8462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7954..7973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8633..9424,9425..9493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9256..9275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9494..9598)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9673..9692)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
      601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
      661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
      721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
      781 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
      841 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
      901 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
      961 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1021 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1081 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1141 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     1201 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     1261 acaaaagtaa gaccaccgca cagcaagggc cgctgatctt cagacctgga ggaggagata
     1321 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     1381 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     1441 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     1501 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     1561 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     1621 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     1681 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     1741 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     1801 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     1861 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     1921 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     1981 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     2041 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     2101 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctacaaatg
     2161 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2221 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2281 aaaattcaaa attttcgggt ttattacagg gacagcagaa attcactttg aattaattca
     2341 agcttcgtga ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     2401 gaagttgggg ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa
     2461 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     2521 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     2581 ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt
     2641 gccttgaatt acttccacct ggctccagta cgtgattctt gatcccgagc tggagccagg
     2701 ggcgggcctt gcgctttagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     2761 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     2821 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     2881 agtcttgtaa atgcgggcca ggatctgcac actggtattt cggtttttgg ggccgcgggc
     2941 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     3001 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     3061 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     3121 tgagcggaaa gatggccgct tcccggccct gctccagggg gctcaaaatg gaggacgcgg
     3181 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaggggcctt tccgtcctca
     3241 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     3301 tggagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     3361 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     3421 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     3481 caaagttttt ttcttccatt tcaggtgtcg tgaacacgct accggtctcg accatggcgg
     3541 ctaccttact agctgctcgg ggagccgggc cagcaccggc ttgggggccg gaggcgttca
     3601 ctccagactg ggaaagccga gaagtttcca ctgggaccac tatcatggcc gtgcagtttg
     3661 acgggggcgt ggttctgggg gcggactcca gaacaaccac tgggtcctac atcgccaatc
     3721 gagtgactga caagctgaca cctattcacg accgcatttt ctgctgtcgc tcaggctcag
     3781 ctgctgatac ccaggcagta gctgatgctg tcacctacca gctcggtttc cacagcattg
     3841 aactgaatga gcctccactg gtccacacag cagccagcct ctttaaggag atgtgttacc
     3901 gataccggga agacctgatg gcgggaatca tcatcgcagg ctgggaccct caagaaggag
     3961 ggcaggtgta ctcagtgcct atggggggta tgatggtaag gcagtccttt gccattggag
     4021 gctccgggag ctcctacatc tatggctatg ttgatgctac ctaccgggaa ggcatgacca
     4081 aggaagagtg tctgcaattc actgccaatg ctctcgcttt ggccatggag cgggatggct
     4141 ccagtggagg agtgatccgc ctggcagcca ttgcagagtc aggggtagag cggcaagtac
     4201 ttttgggaga ccagataccc aaattcgccg ttgccacttt accacccgcc gactacaagg
     4261 acgacgatga caaggcggcc gcggatcctc tagagttaac atcgagggat caagcttatc
     4321 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     4381 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     4441 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     4501 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     4561 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     4621 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     4681 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     4741 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     4801 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     4861 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     4921 cgtcgagacc tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct
     4981 gattgtgcct ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag
     5041 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     5101 aaggggggac tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg
     5161 atctaccaca cacaaggcta cttccctgat tggcagaact acacaccagg gccagggatc
     5221 agatatccac tgacctttgg atggtgctac aagctagtac cagttgagca agagaaggta
     5281 gaagaagcca atgaaggaga gaacacccgc ttgttacacc ctgtgagcct gcatgggatg
     5341 gatgacccgg agagagaagt attagagtgg aggtttgaca gccgcctagc atttcatcac
     5401 atggcccgag agctgcatcc ggactgtact gggtctctct ggttagacca gatctgagcc
     5461 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
     5521 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
     5581 cccttttagt cagtgtggaa aatctctagc agggcccgtt taaacccgct gatcagcctc
     5641 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     5701 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     5761 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     5821 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     5881 aagaaccagc tggggctcta gggggtatcc ccacgcgccc tgtagcggcg cattaagcgc
     5941 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     6001 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     6061 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     6121 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     6181 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     6241 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     6301 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat taattctgtg gaatgtgtgt
     6361 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6421 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6481 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6541 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6601 tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg aggaggcttt
     6661 tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat tttcggatct
     6721 gatcagcacg tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
     6781 aggtgaggaa ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg
     6841 acgtcgccgg agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg
     6901 aggacgactt cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg
     6961 accaggtggt gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt
     7021 acgccgagtg gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga
     7081 ccgagatcgg cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact
     7141 gcgtgcactt cgtggccgag gagcaggact gacacgtgct acgagatttc gattccaccg
     7201 ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc
     7261 tccagcgcgg ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt
     7321 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     7381 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt
     7441 cgacctctag ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     7501 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     7561 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     7621 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     7681 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7741 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7801 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8281 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8461 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8521 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8581 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8641 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8761 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8821 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8881 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8941 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9001 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9061 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9121 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9181 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9241 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9301 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9361 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9421 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9481 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9601 catttccccg aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg
     9661 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc
     9721 ttgtgtgttg gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg
     9781 cttgaccgac aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga
     9841 tgtacgggcc agatatacgc gtt
//
