LOCUS       sequence-17057-e         531 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 531)
  AUTHORS   Rodgers JT, Lerin C, Haas W, Gygi SP, Spiegelman BM, Puigserver P
  TITLE     Nutrient control of glucose homeostasis through a complex of 
            PGC-1alpha and SIRT1.
  JOURNAL   Nature 2005 Mar 3;434(7029):113-8.
  PUBMED    15744310
REFERENCE   2  (bases 1 to 531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature"; 
            date: "2005-03-3"; volume: "434(7029)"; pages: "113-8"
COMMENT     Sequence Label: pAd-Track Flag-SIRT1 H355A
FEATURES             Location/Qualifiers
     source          1..531
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(372..404)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(426..449)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     RBS             459..481
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    496..520
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 gccgccctcg gctcccgccg caggccggac ccattgtctc cgtccccggc cacggccgtc
       61 gccggggcct cccgctccgc gctcgccgcc gcccctgccg cctcccgcca cagcgccgcc
      121 ggggccgcgg cgctcgccgc ctcacacccc gcggccgccg gcgccactgc tgcgctcggc
      181 tcgcccgggc tgcgcccgag gccaggcccg tctcggcggg gcctcttgcg gagcggctcg
      241 tccgccggct gcgacgcggc ctccatggcg gccgccgcgg aaggggagcc ggcggcctga
      301 agggcgagcg ccacctcgtc cgccatcttc caactgcctc tctgtcgacg gagctcgaat
      361 tcggatccgc gacccatttg ctgtccacca gtcatgctag ccatatgcac agatgctctt
      421 cttgctttat cgtcatcgtc tttgtagtcc atggtatatc tccttcttaa agttaaacaa
      481 aattatttct agaggggaat tgttatccgc tcacaattcc cctatagtga g
//
