LOCUS       sequence-170730-        2114 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALE-VP160 construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Gogolok S, Garcia-Diaz C, Pollard SM
  TITLE     STAR: a simple TAL effector assembly reaction using isothermal 
            assembly.
  JOURNAL   Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209.
  PUBMED    27615025
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-12"; volume: "6:33209. doi"; pages: " 
            10.1038/srep33209"
COMMENT     Sequence Label: pTALE-STARVP160-T
FEATURES             Location/Qualifiers
     source          1..2114
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             879..899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             942..1325
                     /codon_start=1
                     /product="10 tandem repeats of the minimal activation 
                     domain of herpes simplex virus VP16 (Cheng et al., 2013)"
                     /label=VP160
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGS
                     DALDDFDLDMLGSDALDDFDLDML"
     CDS             1347..1403
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1404..2114
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1549..1567)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1817..1836
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg
      121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
      181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
      241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
      301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
      361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
      421 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta
      481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gcaggatacg
      541 tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt ggtatcctgc
      601 aggtgctctg tcaggaccac gggcttacgc cacaacaggt ggtggcgatt gcatctaatg
      661 gaggcggacg ccctgccttg gagtccatcg tagcccaatt gtccaggccc gatcccgcgt
      721 tggctgcgtt aacgaatgac catctggtgg cgttggcatg tcttggtgga cgacccgcgc
      781 tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt gatcaaaaga accaaccggc
      841 ggattcccga gagaacttcc catcgagtcg cggctagccc caagaagaag agaaaggtgg
      901 aggccagcgg ttccggacag tggagacggc gggctcgcgc cgacgcgctg gacgatttcg
      961 atctcgacat gctgggttct gatgccctcg atgactttga cctggatatg ttgggaagcg
     1021 acgcattgga tgactttgat ctggacatgc tcggctccga tgctctggac gatttcgatc
     1081 tcgatatgtt agggtcagac gcactggatg atttcgacct tgatatgttg ggaagcgatg
     1141 cccttgatga tttcgacctg gacatgctcg gcagcgacgc cctggacgat ttcgatctgg
     1201 acatgctggg gtccgatgcc ttggatgatt ttgacttgga tatgctgggg agtgatgccc
     1261 tggacgactt tgacctggac atgctgggct ccgatgcgct cgatgacttc gatttggata
     1321 tgttgattaa ctctagaggc agtggagcca ctaacttctc cctgttgaaa caagcagggg
     1381 atgtcgaaga gaatcccggg ccaatggtga gcaagggcga ggaggataac atggccatca
     1441 tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg
     1501 agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg
     1561 tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg
     1621 gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc
     1681 ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga
     1741 cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca
     1801 acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg
     1861 agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga
     1921 aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc
     1981 agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact
     2041 acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg
     2101 agctgtacaa gtaa
//
