LOCUS       sequence-170745-        1151 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Destination vector for TALE-KRAB construction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Gogolok S, Garcia-Diaz C, Pollard SM
  TITLE     STAR: a simple TAL effector assembly reaction using isothermal 
            assembly.
  JOURNAL   Sci Rep. 2016 Sep 12;6:33209. doi: 10.1038/srep33209.
  PUBMED    27615025
REFERENCE   2  (bases 1 to 1151)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-09-12"; volume: "6:33209. doi"; pages: " 
            10.1038/srep33209"
COMMENT     Sequence Label: pTALE-STAR-KRAB-A
FEATURES             Location/Qualifiers
     source          1..1151
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..69
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             76..96
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             879..899
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             954..1148
                     /codon_start=1
                     /product="Kruppel-associated box (KRAB) transcriptional 
                     repression domain from the human zinc finger protein ZNF10 
                     (Margolin et al., 1994)"
                     /label=KRAB
                     /translation="RTLVTFKDVFVDFTREEWKLLDTAQQIVYRNVMLENYKNLVSLGY
                     QLTKPDVILRLEKGEEPWLV"
ORIGIN
        1 atggactaca aagaccatga cggtgattat aaagatcatg acatcgatta caaggatgac
       61 gatgacaaga tggcccccaa gaagaagagg aaggtgggca ttcaccgcgg ggtacctatg
      121 gtggacttga ggacactcgg ttattcgcaa cagcaacagg agaaaatcaa gcctaaggtc
      181 aggagcaccg tcgcgcaaca ccacgaggcg cttgtggggc atggcttcac tcatgcgcat
      241 attgtcgcgc tttcacagca ccctgcggcg cttgggacgg tggctgtcaa ataccaagat
      301 atgattgcgg ccctgcccga agccacgcac gaggcaattg taggggtcgg taaacagtgg
      361 tcgggagcgc gagcacttga ggcgctgctg actgtggcgg gtgagcttag ggggcctccg
      421 ctccagctcg acaccgggca gctgctgaag atcgcgaaga gagggggagt aacagcggta
      481 gaggcagtgc acgcctggcg caatgcgctc accggggccc ccttgaacct gcaggatacg
      541 tttatcagtg ataaagtgtc aagcatgaca aagttgcagc cgaatacagt ggtatcctgc
      601 aggtgctctg tcaggaccac gggcttacgc cacaacaggt ggtggcgatt gcatctaaca
      661 ttggcggacg ccctgccttg gtgtccatcg tagcccaatt gtccaggccc gatcccgcgt
      721 tggctgcgtt aacgaatgac catctggtgg cgttggcatg tcttggtgga cgacccgcgc
      781 tcgatgcagt caaaaagggt ctgcctcatg ctcccgcatt gatcaaaaga accaaccggc
      841 ggattcccga gagaacttcc catcgagtcg cggctagccc caagaagaag agaaaggtgg
      901 aggccagcgg ttccggacag tggatggacg cgaaatcact tacggcatgg tcgagaacac
      961 tggttacgtt caaggacgtg tttgtggact ttacacgtga ggagtggaaa ttgctggata
     1021 ctgcgcaaca aattgtgtat cgaaatgtca tgcttgagaa ttacaagaac ctcgtcagtc
     1081 tcggatacca gttgacgaaa ccggatgtga tccttaggct cgaaaagggg gaagaacctt
     1141 ggctggtata a
//
