LOCUS       sequence-170763-        3617 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Middle entry vector for generating MBS tagged RNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Campbell PD, Chao JA, Singer RH, Marlow FL
  TITLE     Dynamic visualization of transcription and RNA subcellular 
            localization in zebrafish.
  JOURNAL   Development. 2015 Apr 1;142(7):1368-74. doi: 10.1242/dev.118968. 
            Epub 2015 Mar 10.
  PUBMED    25758462
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1242/dev.118968"; journalName: "Development"; date: "2015-04-1- 
            1"; volume: "142"; issue: "7"; pages: "1368-74"
COMMENT     Sequence Label: pME-24xMBS
FEATURES             Location/Qualifiers
     source          1..3617
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     312..331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     565..582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(741..768)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(860..946)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     995..1017
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     1009..1026
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1010..1026
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    1042..1141
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(2479..2578)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2595..2614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2596..2614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2619..2635)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2619..2635)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
       61 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
      121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
      181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
      241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
      301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
      361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
      421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
      481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
      541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
      601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
      661 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
      721 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
      781 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
      841 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
      901 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
      961 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     1021 gccagtctta agctcgggcc ccaaataatg attttatttt gactgatagt gacctgttcg
     1081 ttgcaacaaa ttgatgagca atgctttttt ataatgccaa ctttgtacaa aaaagcaggc
     1141 tccgaattcg cccttcgcgg gatcctacgg tacttattgc caagaaagca cgagcatcag
     1201 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg
     1261 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag
     1321 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc
     1381 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca
     1441 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta
     1501 ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat
     1561 cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag
     1621 cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc
     1681 cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag
     1741 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg
     1801 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag
     1861 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc
     1921 ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt cgaatcttca
     1981 aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct acggtactta
     2041 ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa cgacgacgat
     2101 cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt gccaagaaag
     2161 cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca cgcgtcgctc
     2221 cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca cgagcatcag
     2281 ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca gtattccagg
     2341 gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc gtgcctccag
     2401 gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt tcatcagatc
     2461 tcgcgaaggg cgaattcgac ccagctttct tgtacaaagt tggcattata aaaaataatt
     2521 gctcatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc attatttgcc
     2581 atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt tcctggcagc
     2641 tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2701 gcctcctcta gaccagccag gacagaaatg cctcgacttc gctgctaccc aaggttgccg
     2761 ggtgacgcac accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg
     2821 ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg
     2881 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg
     2941 gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt
     3001 tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttaa
     3061 acattatgag ggaagcggtg atcgccgaag tatcgactca actatcagag gtagttggcg
     3121 tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc tccgcagtgg
     3181 atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc gtaaggcttg
     3241 atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct tcccctggag
     3301 agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac atcattccgt
     3361 ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat gacattcttg
     3421 caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg acaaaagcaa
     3481 gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat ccggttcctg
     3541 aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg ccgcccgact
     3601 gggctggcga tgagcga
//
